First insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from L1 retrotransposons in peanut and wild relative species

Peanut is an allotetraploid (2n = 2x = 40, AABB) of recent origin. Arachis duranensis and A. ipaënsis, the most probable diploid ancestors of the cultigen, and several other wild diploid species with different genomes (A, B, D, F and K) are used in peanut breeding programs. However, the genomic rel...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Samoluk, Sergio Sebastián, Robledo, Germán Ariel, Podio, Maricel, Chalup, Laura María Isabel, Ortíz, Juan Pablo Amelio, Pessino, Silvina Claudia, Seijo, José Guillermo
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2022
Materias:
Arachis genomes
Peanut
LINEs
Reverse transcriptase
Fluorescent in situ hybridization
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/30851
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-30851
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Arachis genomes
Peanut
LINEs
Reverse transcriptase
Fluorescent in situ hybridization
spellingShingle Arachis genomes
Peanut
LINEs
Reverse transcriptase
Fluorescent in situ hybridization
Samoluk, Sergio Sebastián
Robledo, Germán Ariel
Podio, Maricel
Chalup, Laura María Isabel
Ortíz, Juan Pablo Amelio
Pessino, Silvina Claudia
Seijo, José Guillermo
First insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from L1 retrotransposons in peanut and wild relative species
topic_facet Arachis genomes
Peanut
LINEs
Reverse transcriptase
Fluorescent in situ hybridization
description Peanut is an allotetraploid (2n = 2x = 40, AABB) of recent origin. Arachis duranensis and A. ipaënsis, the most probable diploid ancestors of the cultigen, and several other wild diploid species with different genomes (A, B, D, F and K) are used in peanut breeding programs. However, the genomic relationships and the evolutionary pathways of genome differentiation of these species are poorly understood. We performed a sequencebased phylogenetic analysis of the L1 reverse transcriptase and estimated its representation and chromosome distribution in species of five genomes and three karyotype groups with the aim of contributing to the knowledge of the genomic structure and evolution of peanut and wild diploid relatives. All the isolated rt fragments were found to belong to plant L1 lineage and were named ALI. The best supported phylogenetic groups were not concordant with the genomes or karyotype groups. The copy number of ALI sequences was higher than the expected one for plants and directly related to genome size. FISH experiments revealed that ALI is mainly located on the euchromatin of interstitial and distal regions of most chromosome arms. Divergence of ALI sequences would have occurred before the differentiation of the genomes and karyotype groups of Arachis. The representation and chromosome distribution of ALI in peanut was almost additive of those of the parental species suggesting that the spontaneous hybridization of the two parental species of peanut followed by chromosome doubling would not have induced a significant burst of ALI transposition.
format Artículo
author Samoluk, Sergio Sebastián
Robledo, Germán Ariel
Podio, Maricel
Chalup, Laura María Isabel
Ortíz, Juan Pablo Amelio
Pessino, Silvina Claudia
Seijo, José Guillermo
author_facet Samoluk, Sergio Sebastián
Robledo, Germán Ariel
Podio, Maricel
Chalup, Laura María Isabel
Ortíz, Juan Pablo Amelio
Pessino, Silvina Claudia
Seijo, José Guillermo
author_sort Samoluk, Sergio Sebastián
title First insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from L1 retrotransposons in peanut and wild relative species
title_short First insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from L1 retrotransposons in peanut and wild relative species
title_full First insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from L1 retrotransposons in peanut and wild relative species
title_fullStr First insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from L1 retrotransposons in peanut and wild relative species
title_full_unstemmed First insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from L1 retrotransposons in peanut and wild relative species
title_sort first insight into divergence, representation and chromosome distribution of reverse transcriptase fragments from l1 retrotransposons in peanut and wild relative species
publisher Springer
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/30851
work_keys_str_mv AT samoluksergiosebastian firstinsightintodivergencerepresentationandchromosomedistributionofreversetranscriptasefragmentsfroml1retrotransposonsinpeanutandwildrelativespecies
AT robledogermanariel firstinsightintodivergencerepresentationandchromosomedistributionofreversetranscriptasefragmentsfroml1retrotransposonsinpeanutandwildrelativespecies
AT podiomaricel firstinsightintodivergencerepresentationandchromosomedistributionofreversetranscriptasefragmentsfroml1retrotransposonsinpeanutandwildrelativespecies
AT chaluplauramariaisabel firstinsightintodivergencerepresentationandchromosomedistributionofreversetranscriptasefragmentsfroml1retrotransposonsinpeanutandwildrelativespecies
AT ortizjuanpabloamelio firstinsightintodivergencerepresentationandchromosomedistributionofreversetranscriptasefragmentsfroml1retrotransposonsinpeanutandwildrelativespecies
AT pessinosilvinaclaudia firstinsightintodivergencerepresentationandchromosomedistributionofreversetranscriptasefragmentsfroml1retrotransposonsinpeanutandwildrelativespecies
AT seijojoseguillermo firstinsightintodivergencerepresentationandchromosomedistributionofreversetranscriptasefragmentsfroml1retrotransposonsinpeanutandwildrelativespecies
bdutipo_str Repositorios
_version_ 1764820539299332097

Ejemplares similares