Efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection

Bothrops alternatus snake venom is particularly characterized for inducing a prominent haemorrhage and affecting hemostasis as a consequence of 43.1% of metallo-proteinases and less than 10% of PLA2 (almost all non-myotoxic phospholipases) in its venomics. In addition, myonecrosis is the major loc...

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Autores principales: García Denegri, María Emilia, Teibler, Gladys Pamela, Maruñak, Silvana L., Hernández, David Roque, Acosta, Ofelia Cristina, Leiva, Laura Cristina
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2021
Materias:
Efficient
Haemorrhagic
venom
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/30352
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-30352
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Efficient
Haemorrhagic
venom
spellingShingle Efficient
Haemorrhagic
venom
García Denegri, María Emilia
Teibler, Gladys Pamela
Maruñak, Silvana L.
Hernández, David Roque
Acosta, Ofelia Cristina
Leiva, Laura Cristina
Efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection
topic_facet Efficient
Haemorrhagic
venom
description Bothrops alternatus snake venom is particularly characterized for inducing a prominent haemorrhage and affecting hemostasis as a consequence of 43.1% of metallo-proteinases and less than 10% of PLA2 (almost all non-myotoxic phospholipases) in its venomics. In addition, myonecrosis is the major local effect in viper envenoming which might lead to permanent sequela. Then, the rebuilding of the microvasculature at the local injured site acquires significance since represents one of the pivotal stages for subsequent skeletal muscle regeneration either at morphological or functional aspects. Due to the significance played by vasculature in this process, it is important to study by histology and immunohistochemical tech niques, the muscular damage and the sequence of skeletal muscle reconstruction (degree of damage, reconstitution of muscle fibres and capillaries). In this work, we injected intramuscularly 50 or 100 mg per mouse of B. alternatus venom in gastrocnemius muscles. We provided a complete description and characterization of the different stages of myogenesis after mild (50 mg) and severe (100 mg) local injury induced by B. alternatus venom toxins. The regeneration was evaluated 24 h, 3, 7, 14 and 28 days after receiving venom injection. Finally, both doses induced an extended necrosis at the site of injection where, when critical steps in the regenerative process are taking place, an efficient tissue rebuilding is achieved. B. alternatus venom is characterized by the high percentage of exclusively class P-III metal loproteinases, and by the lack of class P-I metalloproteinases in its venom composition. This could explain the effectiveness of muscle regeneration after venom injection despite the severity of the initial phase of envenoming.
format Artículo
author García Denegri, María Emilia
Teibler, Gladys Pamela
Maruñak, Silvana L.
Hernández, David Roque
Acosta, Ofelia Cristina
Leiva, Laura Cristina
author_facet García Denegri, María Emilia
Teibler, Gladys Pamela
Maruñak, Silvana L.
Hernández, David Roque
Acosta, Ofelia Cristina
Leiva, Laura Cristina
author_sort García Denegri, María Emilia
title Efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection
title_short Efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection
title_full Efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection
title_fullStr Efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection
title_full_unstemmed Efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection
title_sort efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection
publisher Elsevier
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/30352
work_keys_str_mv AT garciadenegrimariaemilia efficientmuscleregenerationafterhighlyhaemorrhagicbothropsalternatusvenominjection
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AT marunaksilvanal efficientmuscleregenerationafterhighlyhaemorrhagicbothropsalternatusvenominjection
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bdutipo_str Repositorios
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