Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species
Satellite DNA (satDNA) is a major component of the heterochromatic regions of eukaryote genomes and usually shows a high evolutionary dynamic, even among closely related species. Section Arachis (genus Arachis) is composed of species belonging to six different genomes (A, B, D, F, G and K). The...
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Springer
2021
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| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/30270 |
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I48-R184-123456789-302702024-10-07T10:35:17Z Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species Samoluk, Sergio Sebastián Robledo Dobladez, Germán Ariel Bertioli, David J. Seijo, José Guillermo Satellite DNA Library hypothesis Heterochromatin Karyotype differentiation Arachis species Satellite DNA (satDNA) is a major component of the heterochromatic regions of eukaryote genomes and usually shows a high evolutionary dynamic, even among closely related species. Section Arachis (genus Arachis) is composed of species belonging to six different genomes (A, B, D, F, G and K). The most distinguishing features among these genomes are the amount and distribution of the heterochromatin in the karyotypes. With the objective of gaining insight into the sequence composition and evolutionary dynamics of the heterochromatin fraction in Arachis, we investigated here the sequence diversity, genomic abundance, and chromosomal distribution of a satDNA family (ATR-2) among seven diploid species of section Arachis. All of the isolated sequences were AT-rich and highly conserved at both intraspecific and interspecific levels, without any species-specific polymorphism. Pairwise comparisons of isolated ATR-2 monomers revealed that most of the nucleotide sites were in the first two transitional stages of Strachan’s model. However, the abundance of ATR-2 was significantly different among genomes according to the ‘library hypothesis’. Fluorescent in situ hybridization revealed that ATR-2 is a main component of the DAPI+ centromeric heterochromatin of the A, F, and K genomes. Thus, the evolution of the different heterochromatin patterns observed in Arachis genomes can be explained, at least in part, by the differential representation of ATR-2 among the different species or even among the chromosomes of the same complement. These findings are the first to demonstrate the participation of satDNA sequences in the karyotype diversification of wild diploid Arachis species. 2021-12-02T19:57:48Z 2021-12-02T19:57:48Z 2016 Artículo Samoluk, Sergio Sebastián, et al., 2016. Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species. Molecular Genetics And Genomics. Heidelberg: Springer, vol. 292, no. 2, p. 283-296. ISSN 1617-4615. 1617-4615 http://repositorio.unne.edu.ar/handle/123456789/30270 eng https://link.springer.com/article/10.1007/s00438-016-1271-3 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Springer Molecular Genetics And Genomics, 2016, vol. 292, no. 2, p. 283-296. |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-184 |
| collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
| language |
Inglés |
| topic |
Satellite DNA Library hypothesis Heterochromatin Karyotype differentiation Arachis species |
| spellingShingle |
Satellite DNA Library hypothesis Heterochromatin Karyotype differentiation Arachis species Samoluk, Sergio Sebastián Robledo Dobladez, Germán Ariel Bertioli, David J. Seijo, José Guillermo Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species |
| topic_facet |
Satellite DNA Library hypothesis Heterochromatin Karyotype differentiation Arachis species |
| description |
Satellite DNA (satDNA) is a major component
of the heterochromatic regions of eukaryote genomes and
usually shows a high evolutionary dynamic, even among
closely related species. Section Arachis (genus Arachis) is
composed of species belonging to six different genomes
(A, B, D, F, G and K). The most distinguishing features
among these genomes are the amount and distribution of
the heterochromatin in the karyotypes. With the objective
of gaining insight into the sequence composition and evolutionary
dynamics of the heterochromatin fraction in Arachis,
we investigated here the sequence diversity, genomic abundance, and chromosomal distribution of a satDNA
family (ATR-2) among seven diploid species of section
Arachis. All of the isolated sequences were AT-rich and
highly conserved at both intraspecific and interspecific
levels, without any species-specific polymorphism. Pairwise
comparisons of isolated ATR-2 monomers revealed
that most of the nucleotide sites were in the first two transitional
stages of Strachan’s model. However, the abundance
of ATR-2 was significantly different among genomes
according to the ‘library hypothesis’. Fluorescent in situ
hybridization revealed that ATR-2 is a main component of
the DAPI+ centromeric heterochromatin of the A, F, and
K genomes. Thus, the evolution of the different heterochromatin
patterns observed in Arachis genomes can be
explained, at least in part, by the differential representation
of ATR-2 among the different species or even among
the chromosomes of the same complement. These findings
are the first to demonstrate the participation of satDNA
sequences in the karyotype diversification of wild diploid
Arachis species. |
| format |
Artículo |
| author |
Samoluk, Sergio Sebastián Robledo Dobladez, Germán Ariel Bertioli, David J. Seijo, José Guillermo |
| author_facet |
Samoluk, Sergio Sebastián Robledo Dobladez, Germán Ariel Bertioli, David J. Seijo, José Guillermo |
| author_sort |
Samoluk, Sergio Sebastián |
| title |
Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species |
| title_short |
Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species |
| title_full |
Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species |
| title_fullStr |
Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species |
| title_full_unstemmed |
Evolutionary dynamics of an at‑rich satellite DNA and its contribution to karyotype differentiation in wild diploid arachis species |
| title_sort |
evolutionary dynamics of an at‑rich satellite dna and its contribution to karyotype differentiation in wild diploid arachis species |
| publisher |
Springer |
| publishDate |
2021 |
| url |
http://repositorio.unne.edu.ar/handle/123456789/30270 |
| work_keys_str_mv |
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| _version_ |
1832345676657197056 |