Transcriptome analysis of seed development in apomictic Paspalum notatum

The seed developmental process involves various tissues with several ploidy levels and different genetic origins. Therefore, its characterisation at the transcriptome level is certainly a challenge. The hypothesis of endosperm balance number (EBN) postulates that each species has an effective number...

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Autores principales: Felitti, Silvina Andrea, Acuña, Carlos Alberto, Ortíz, Juan Pablo Amelio, Quarin, Camilo Luis
Formato: Artículo
Lenguaje:Inglés
Publicado: Ricardo Antunes Azevedo 2021
Materias:
Endosperm
Endosperm balance number
Extracellular ATP
Paspalum notatum
Pseudogamy
Transcriptome
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28630
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28630
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Endosperm
Endosperm balance number
Extracellular ATP
Paspalum notatum
Pseudogamy
Transcriptome
spellingShingle Endosperm
Endosperm balance number
Extracellular ATP
Paspalum notatum
Pseudogamy
Transcriptome
Felitti, Silvina Andrea
Acuña, Carlos Alberto
Ortíz, Juan Pablo Amelio
Quarin, Camilo Luis
Transcriptome analysis of seed development in apomictic Paspalum notatum
topic_facet Endosperm
Endosperm balance number
Extracellular ATP
Paspalum notatum
Pseudogamy
Transcriptome
description The seed developmental process involves various tissues with several ploidy levels and different genetic origins. Therefore, its characterisation at the transcriptome level is certainly a challenge. The hypothesis of endosperm balance number (EBN) postulates that each species has an effective number that is important for normal endosperm and seed development to occur. Understanding endosperm formation in apomictic plants is crucial for the perspective of transferring apomixis to sexual species of agronomic interest. Since sexual tetraploid Paspalum plants fit the EBN premise, the EBN insensitivity observed in apomictic plants might be a requirement for the spread of pseudogamous apomixis. Crosses using several cytotypes of Paspalum notatum were made in order to induce the development of seeds with different maternal/paternal genomic ratios in the endosperm. A transcriptome characterisation of ovaries 3h after pollination was performed using cDNA-AFLP methodology. Forty-six of the 100 differentially expressed transcript-derived fragments (DETDFs) were specifically found in crosses in which apomictic plants were used as the female parent and presented a predicted m:p ratio in the endosperm that was different to the 2:1 requirement of the EBN. Moreover, 12 of the DETDFs presented identity with proteins that were differentially expressed in response to changes in the levels of extracellular ATP (eATP) in Arabidopsis cell suspension cultures. eATP is an important molecular switch in plants that tightly controls organellar energy metabolism and activates gene expression controlling specific growth and developmental programmes. The results suggest that eATP-mediated signalling could be involved in the regulation of endosperm development.
format Artículo
author Felitti, Silvina Andrea
Acuña, Carlos Alberto
Ortíz, Juan Pablo Amelio
Quarin, Camilo Luis
author_facet Felitti, Silvina Andrea
Acuña, Carlos Alberto
Ortíz, Juan Pablo Amelio
Quarin, Camilo Luis
author_sort Felitti, Silvina Andrea
title Transcriptome analysis of seed development in apomictic Paspalum notatum
title_short Transcriptome analysis of seed development in apomictic Paspalum notatum
title_full Transcriptome analysis of seed development in apomictic Paspalum notatum
title_fullStr Transcriptome analysis of seed development in apomictic Paspalum notatum
title_full_unstemmed Transcriptome analysis of seed development in apomictic Paspalum notatum
title_sort transcriptome analysis of seed development in apomictic paspalum notatum
publisher Ricardo Antunes Azevedo
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28630
work_keys_str_mv AT felittisilvinaandrea transcriptomeanalysisofseeddevelopmentinapomicticpaspalumnotatum
AT acunacarlosalberto transcriptomeanalysisofseeddevelopmentinapomicticpaspalumnotatum
AT ortizjuanpabloamelio transcriptomeanalysisofseeddevelopmentinapomicticpaspalumnotatum
AT quarincamiloluis transcriptomeanalysisofseeddevelopmentinapomicticpaspalumnotatum
bdutipo_str Repositorios
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