Genetic response of Paspalum plicatulum to genome duplication

Paspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained thr...

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Detalles Bibliográficos
Autores principales: Weihmüller, Emilse, Beltrán, Celina, Sartor, María Esperanza, Espinoza, Francisco, Spampinato, Claudia, Pessino, Silvina Claudia
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer International Publishing 2021
Materias:
Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28624
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28624
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
spellingShingle Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
Weihmüller, Emilse
Beltrán, Celina
Sartor, María Esperanza
Espinoza, Francisco
Spampinato, Claudia
Pessino, Silvina Claudia
Genetic response of Paspalum plicatulum to genome duplication
topic_facet Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
description Paspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained through colchicine treatment of seeds generated by open pollination of a diploid plant (H14-2x). Diploid and tetraploid sectors from both mixoploids were dissected to generate two ploidy series (4C- 2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were generated from the maternal plant H14-2x, both ploidy series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid plant (C1) produced by selfing 7D-4x. Our results indicate that immediately after polyploidization P. plicatulum suffers genetic rearrangements affecting *28–38 % of the genome. Band gain and loss were equally prevalent at a statistically significant level. At least 5.62 % of the genome experimented recurrent genetic variation in a non-random basis with a confidence of 94.88 %. A significant proportion of novel bands (36 out of 195; 18.4 %) was detected in the C1 tetraploid plant. Half of these bands were not amplified in either H14-2x or 7D-4x, while the remainders were present in H14-2x but absent in 7D-4x. Our results indicate the occurrence of a considerable number of genetic changes in P. plicatulum immediately after polyploidization, some of which were recurrently detected in different independent events. Moreover, we confirmed that after polyploidization, lost ancestral alleles were spontaneously recovered in further generations, a phenomenon previously reported by other research groups.
format Artículo
author Weihmüller, Emilse
Beltrán, Celina
Sartor, María Esperanza
Espinoza, Francisco
Spampinato, Claudia
Pessino, Silvina Claudia
author_facet Weihmüller, Emilse
Beltrán, Celina
Sartor, María Esperanza
Espinoza, Francisco
Spampinato, Claudia
Pessino, Silvina Claudia
author_sort Weihmüller, Emilse
title Genetic response of Paspalum plicatulum to genome duplication
title_short Genetic response of Paspalum plicatulum to genome duplication
title_full Genetic response of Paspalum plicatulum to genome duplication
title_fullStr Genetic response of Paspalum plicatulum to genome duplication
title_full_unstemmed Genetic response of Paspalum plicatulum to genome duplication
title_sort genetic response of paspalum plicatulum to genome duplication
publisher Springer International Publishing
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28624
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AT espinozafrancisco geneticresponseofpaspalumplicatulumtogenomeduplication
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bdutipo_str Repositorios
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