Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide

USP, EP, and Argentinian Pharmacopoeia proposed HPLC-UV for quantitative quality control of mebendazole (MBZ) tablets. In this work, a spectrophotometric method is proposed. A mebendazole solution was prepared by dissolving the active ingredient in an ethanolic solution of HCl (1: 100) and adding Na...

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Autores principales: Delfino, Mario Raúl, Monzón, Celina María, Jorge, Nelly Lidia, Sarno, María del Carmen Teresa
Formato: Artículo
Lenguaje:Inglés
Publicado: Asian Journal of Science and Technology 2021
Materias:
Mebendazole
Quality control
Spectrophotometric method
Validation
Benzimidazoles
Alternative method
Theoretical study
Interaction products-static
Approximation
Density functional theory
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28012
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28012
record_format dspace
spelling I48-R184-123456789-280122025-03-06T10:58:24Z Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide Delfino, Mario Raúl Monzón, Celina María Jorge, Nelly Lidia Sarno, María del Carmen Teresa Mebendazole Quality control Spectrophotometric method Validation Benzimidazoles Alternative method Theoretical study Interaction products-static Approximation Density functional theory USP, EP, and Argentinian Pharmacopoeia proposed HPLC-UV for quantitative quality control of mebendazole (MBZ) tablets. In this work, a spectrophotometric method is proposed. A mebendazole solution was prepared by dissolving the active ingredient in an ethanolic solution of HCl (1: 100) and adding NaOH 3N. It was allowed to stand 10 minutes. Absorbance spectrum was scanned between 350 and 700 nm. Maximum was found at 400 nm. A calibration curve in the range of 0.05 to 0.25 mg / mL, responded to A = (2.2746 ± 0.0224) C + (0.0012 ± 0.0068) with R2 = 0.9999. The RSD% was 0.961 indicating good repeatability for the analytical procedure. Accuracy in recovery experience was found to be 99.2 - 100.6%. Statistical comparison using t -test and F –test indicate that there are no significant differences between HPLC and the spectrophometric methods, whith a 95% confidence level. Specificity and intermediate precision assays were satisfactory. Quantum theoretical chemistry was applied to elucidate the interaction that gives origin to the color, using static approximation and density functional theory, B3LYP and base 6-311G (d, p). Excitation energies B3LYP for MBZ and MBZ product of the interaction with sodium atom, were coincidental with experimental results. 2021-06-01T12:41:32Z 2021-06-01T12:41:32Z 2016-11-30 Artículo Delfino, Mario Raúl, et. al., 2016. Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide. Asian Journal of Science and Technology. India: Asian Journal of Science and Technology, vol. 07, no. 11, p. 3948-3953. ISSN 0976-3376. 0976-3376 http://repositorio.unne.edu.ar/handle/123456789/28012 eng https://www.journalajst.com/mebendazole-spectrophotometric-determination-theoretical-and-experimental-study-interaction-sodium openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Asian Journal of Science and Technology Asian Journal of Science and Technology, 2016, vol. 07, no. 11, p. 3948-3953
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Mebendazole
Quality control
Spectrophotometric method
Validation
Benzimidazoles
Alternative method
Theoretical study
Interaction products-static
Approximation
Density functional theory
spellingShingle Mebendazole
Quality control
Spectrophotometric method
Validation
Benzimidazoles
Alternative method
Theoretical study
Interaction products-static
Approximation
Density functional theory
Delfino, Mario Raúl
Monzón, Celina María
Jorge, Nelly Lidia
Sarno, María del Carmen Teresa
Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide
topic_facet Mebendazole
Quality control
Spectrophotometric method
Validation
Benzimidazoles
Alternative method
Theoretical study
Interaction products-static
Approximation
Density functional theory
description USP, EP, and Argentinian Pharmacopoeia proposed HPLC-UV for quantitative quality control of mebendazole (MBZ) tablets. In this work, a spectrophotometric method is proposed. A mebendazole solution was prepared by dissolving the active ingredient in an ethanolic solution of HCl (1: 100) and adding NaOH 3N. It was allowed to stand 10 minutes. Absorbance spectrum was scanned between 350 and 700 nm. Maximum was found at 400 nm. A calibration curve in the range of 0.05 to 0.25 mg / mL, responded to A = (2.2746 ± 0.0224) C + (0.0012 ± 0.0068) with R2 = 0.9999. The RSD% was 0.961 indicating good repeatability for the analytical procedure. Accuracy in recovery experience was found to be 99.2 - 100.6%. Statistical comparison using t -test and F –test indicate that there are no significant differences between HPLC and the spectrophometric methods, whith a 95% confidence level. Specificity and intermediate precision assays were satisfactory. Quantum theoretical chemistry was applied to elucidate the interaction that gives origin to the color, using static approximation and density functional theory, B3LYP and base 6-311G (d, p). Excitation energies B3LYP for MBZ and MBZ product of the interaction with sodium atom, were coincidental with experimental results.
format Artículo
author Delfino, Mario Raúl
Monzón, Celina María
Jorge, Nelly Lidia
Sarno, María del Carmen Teresa
author_facet Delfino, Mario Raúl
Monzón, Celina María
Jorge, Nelly Lidia
Sarno, María del Carmen Teresa
author_sort Delfino, Mario Raúl
title Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide
title_short Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide
title_full Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide
title_fullStr Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide
title_full_unstemmed Mebendazole spectrophotometric determination. Theoretical and experimental study of the interaction with sodium hydroxide
title_sort mebendazole spectrophotometric determination. theoretical and experimental study of the interaction with sodium hydroxide
publisher Asian Journal of Science and Technology
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28012
work_keys_str_mv AT delfinomarioraul mebendazolespectrophotometricdeterminationtheoreticalandexperimentalstudyoftheinteractionwithsodiumhydroxide
AT monzoncelinamaria mebendazolespectrophotometricdeterminationtheoreticalandexperimentalstudyoftheinteractionwithsodiumhydroxide
AT jorgenellylidia mebendazolespectrophotometricdeterminationtheoreticalandexperimentalstudyoftheinteractionwithsodiumhydroxide
AT sarnomariadelcarmenteresa mebendazolespectrophotometricdeterminationtheoreticalandexperimentalstudyoftheinteractionwithsodiumhydroxide
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