Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare a traditional tea-like infusion (mate-tea) with various medicinal benefits....

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Autores principales: Acevedo, Raúl Maximiliano, Ruiz, Oscar Adolfo, Sansberro, Pedro Alfonso
Formato: Artículo
Lenguaje:Inglés
Publicado: Southerm Cross 2021
Materias:
Abiotic stress
Gene expression
mRNA Differential Display
Water deficit
Yerba mate
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27841
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27841
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Abiotic stress
Gene expression
mRNA Differential Display
Water deficit
Yerba mate
spellingShingle Abiotic stress
Gene expression
mRNA Differential Display
Water deficit
Yerba mate
Acevedo, Raúl Maximiliano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
Gene expression changes in response to drought stress in Ilex paraguariensis leaves
topic_facet Abiotic stress
Gene expression
mRNA Differential Display
Water deficit
Yerba mate
description Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare a traditional tea-like infusion (mate-tea) with various medicinal benefits. Under the prevailing agro-climatic conditions in NE Argentina and with the occurrence of high temperatures and scarce rains during spring or summer, I. paraguariensis plants are subjected to water stress, reducing their yield. Differential Display was used to compare differences in gene expression between stressed and unstressed (control) potted plants. We found 52 differentially expressed transcripts (DETs), analysing banding patterns of Differential Display polyacrylamide gels, in which 38 DETs were induced, and 14 DETs were repressed in response to drought. Sequence comparisons revealed that 19% of the fragments showed no homologies, whereas 40.5 % of the total number of fragments had strong similarities with database entries of known functions. The other DETs belonged to unclassified proteins or genes with unknown functions. Interestingly, 12% of the DNA sequences were related to signal transduction, and 2% of the sequences were identified as transcription factors. Approximately, 12% of the annotated sequences were involved in stress tolerance and metal homeostasis, while 7% of the DETs were associated with photosynthesis. Other groups, representing 12%, included those involved in secondary metabolism, lignin biosynthesis, cell walls and RNA stabilization/chaperone activity. When integrated with physiological data, our results suggest that the transcriptional activity of some of the studied genes could be related to the degree of drought tolerance/susceptibility of I. paraguariensis.
format Artículo
author Acevedo, Raúl Maximiliano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_facet Acevedo, Raúl Maximiliano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_sort Acevedo, Raúl Maximiliano
title Gene expression changes in response to drought stress in Ilex paraguariensis leaves
title_short Gene expression changes in response to drought stress in Ilex paraguariensis leaves
title_full Gene expression changes in response to drought stress in Ilex paraguariensis leaves
title_fullStr Gene expression changes in response to drought stress in Ilex paraguariensis leaves
title_full_unstemmed Gene expression changes in response to drought stress in Ilex paraguariensis leaves
title_sort gene expression changes in response to drought stress in ilex paraguariensis leaves
publisher Southerm Cross
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/27841
work_keys_str_mv AT acevedoraulmaximiliano geneexpressionchangesinresponsetodroughtstressinilexparaguariensisleaves
AT ruizoscaradolfo geneexpressionchangesinresponsetodroughtstressinilexparaguariensisleaves
AT sansberropedroalfonso geneexpressionchangesinresponsetodroughtstressinilexparaguariensisleaves
bdutipo_str Repositorios
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