Tolerance to desiccation and cryopreservation of seeds of seven south american llex species

This research aimed at evaluating the desiccation tolerance and ability to withstand cryostorage of intact seeds of seven South American Ilex species, and comparing different methodologies for in vitro germination of fresh and cryostored seeds. Intact seeds were silica gel–desiccated from 2 to 14 ho...

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Autores principales: Dolce, Natalia Raquel, Medina, Ricardo Daniel, González Arnao, María Teresa
Formato: Artículo
Lenguaje:Inglés
Publicado: American society for horticultural science 2020
Materias:
Long-term storage
Liquid nitrogen
Seed bank
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27533
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27533
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Long-term storage
Liquid nitrogen
Seed bank
spellingShingle Long-term storage
Liquid nitrogen
Seed bank
Dolce, Natalia Raquel
Medina, Ricardo Daniel
González Arnao, María Teresa
Tolerance to desiccation and cryopreservation of seeds of seven south american llex species
topic_facet Long-term storage
Liquid nitrogen
Seed bank
description This research aimed at evaluating the desiccation tolerance and ability to withstand cryostorage of intact seeds of seven South American Ilex species, and comparing different methodologies for in vitro germination of fresh and cryostored seeds. Intact seeds were silica gel–desiccated from 2 to 14 hours, placed in cryovials, and immersed in liquid nitrogen (LN). Survival was assessed through in vitro germination of intact seeds, bisected seeds, or isolated embryos. Seeds of the seven Ilex species (Ilex brasiliensis (Sprengel) Loes., Ilex brevicuspis R., Ilex dumosa var. dumosa R., Ilex integerrima (Vell. Conc.) R., Ilex paraguariensis A. St. Hil., Ilex pseudoboxus R., and Ilex theezans R.) tolerated desiccation to ’6% moisture content (MC) and could be successfully cryopreserved when MC decreased between 6.4% and 8.4% depending on the species, before immersion in LN. In addition, it was established as the optimal condition for in vitro seed germination of the seven Ilex species. A simple and costeffective cryogenic procedure (which did not require the use of cryoprotectants or sophisticated facilities) was defined for seeds of seven Ilex species, which provides a new alternative for safe long-term preservation of Ilex germplasm.
format Artículo
author Dolce, Natalia Raquel
Medina, Ricardo Daniel
González Arnao, María Teresa
author_facet Dolce, Natalia Raquel
Medina, Ricardo Daniel
González Arnao, María Teresa
author_sort Dolce, Natalia Raquel
title Tolerance to desiccation and cryopreservation of seeds of seven south american llex species
title_short Tolerance to desiccation and cryopreservation of seeds of seven south american llex species
title_full Tolerance to desiccation and cryopreservation of seeds of seven south american llex species
title_fullStr Tolerance to desiccation and cryopreservation of seeds of seven south american llex species
title_full_unstemmed Tolerance to desiccation and cryopreservation of seeds of seven south american llex species
title_sort tolerance to desiccation and cryopreservation of seeds of seven south american llex species
publisher American society for horticultural science
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/27533
work_keys_str_mv AT dolcenataliaraquel tolerancetodesiccationandcryopreservationofseedsofsevensouthamericanllexspecies
AT medinaricardodaniel tolerancetodesiccationandcryopreservationofseedsofsevensouthamericanllexspecies
AT gonzalezarnaomariateresa tolerancetodesiccationandcryopreservationofseedsofsevensouthamericanllexspecies
bdutipo_str Repositorios
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