Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide

Cyclophosphamide is a cytostatic agent used in conditioning regimens for bone marrow transplantation and in some solid tumors. It has the capacity to induce alterations in several organ systems, particularly the stomatognathic system. Previous studies by our team demonstrated an oxidative and functi...

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Autores principales:	Wietz, Fernando Martín, Bachmeier, Evelin, Fonseca Acosta, Ismael Bernardo, Ávila Uliarte, Rodolfo Esteban, Samar Romani, María Elena, Mazzeo, Marcelo Adrián
Formato:	Artículo revista
Lenguaje:	Español
Publicado:	Facultad de Odontología de la Universidad Nacional del Nordeste (FOUNNE) 2025
Materias:	cyclophosphamide melatonin submandibular gland Wistar rats ciclofosfamida melatonina glándula submandibular ratas Wistar glândula submandibular ratos Wistar
Acceso en línea:	https://revistas.unne.edu.ar/index.php/rfo/article/view/9030
Aporte de:	Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste

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institution	Universidad Nacional del Nordeste
institution_str	I-48
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container_title_str	Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language	Español
format	Artículo revista
topic	cyclophosphamide melatonin submandibular gland Wistar rats ciclofosfamida melatonina glándula submandibular ratas Wistar ciclofosfamida melatonina glândula submandibular ratos Wistar
spellingShingle	cyclophosphamide melatonin submandibular gland Wistar rats ciclofosfamida melatonina glándula submandibular ratas Wistar ciclofosfamida melatonina glândula submandibular ratos Wistar Wietz, Fernando Martín Bachmeier, Evelin Fonseca Acosta, Ismael Bernardo Ávila Uliarte, Rodolfo Esteban Samar Romani, María Elena Mazzeo, Marcelo Adrián Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
topic_facet	cyclophosphamide melatonin submandibular gland Wistar rats ciclofosfamida melatonina glándula submandibular ratas Wistar ciclofosfamida melatonina glândula submandibular ratos Wistar
author	Wietz, Fernando Martín Bachmeier, Evelin Fonseca Acosta, Ismael Bernardo Ávila Uliarte, Rodolfo Esteban Samar Romani, María Elena Mazzeo, Marcelo Adrián
author_facet	Wietz, Fernando Martín Bachmeier, Evelin Fonseca Acosta, Ismael Bernardo Ávila Uliarte, Rodolfo Esteban Samar Romani, María Elena Mazzeo, Marcelo Adrián
author_sort	Wietz, Fernando Martín
title	Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
title_short	Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
title_full	Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
title_fullStr	Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
title_full_unstemmed	Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
title_sort	structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
description	Cyclophosphamide is a cytostatic agent used in conditioning regimens for bone marrow transplantation and in some solid tumors. It has the capacity to induce alterations in several organ systems, particularly the stomatognathic system. Previous studies by our team demonstrated an oxidative and functional effect of this drug, assessed through increased superoxide dismutase, uric acid, and glycogen levels in rat submandibular gland. On the other hand, melatonin is known for its multipurpose action due to its capacity to attenuate structural and functional alterations in several organs with high functional activity and mitotic rates. In the present study, we evaluated histological changes in ducts and acini of the submandibular gland of rats treated with cyclophosphamide, as well as the protective effect of exogenously administered melatonin. Three-month-old adult male Wistar rats with body weight ranging from 300 to 350 grams, were used and divided into five experimental groups: 1) C: Control, 2) Control and Ethanol, 3) Cyclophosphamide, 4) Cyclophosphamide and Melatonin, and 5) Melatonin. Histological sections of submandibular glands were prepared and stained with Hematoxylin/Eosin, where possible structural changes in ducts and acini were analyzed. Glands from animals treated with cyclophosphamide showed acinar cells with macrokaryosis and hyperchromasia, binucleation with irregular contours, prominent nucleoli, and perinuclear chromatin clumps compared to control groups. It is inferred that melatonin administration at the tested dose could attenuate the structural alterations of the submandibular gland treated with this chemotherapeutic agent. The reversal of such changes would explain the reduction of antioxidant and functional effects at the glandular level described in our laboratory.
publisher	Facultad de Odontología de la Universidad Nacional del Nordeste (FOUNNE)
publishDate	2025
url	https://revistas.unne.edu.ar/index.php/rfo/article/view/9030
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spelling	I48-R154-article-90302025-12-29T12:20:55Z Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide Análisis estructural del efecto de melatonina en glándula submandibular de ratas tratadas con ciclofosfamida Análise estrutural do efeito da melatonina na glândula submandibular de ratos tratados com ciclofosfamida Wietz, Fernando Martín Bachmeier, Evelin Fonseca Acosta, Ismael Bernardo Ávila Uliarte, Rodolfo Esteban Samar Romani, María Elena Mazzeo, Marcelo Adrián cyclophosphamide melatonin submandibular gland Wistar rats ciclofosfamida melatonina glándula submandibular ratas Wistar ciclofosfamida melatonina glândula submandibular ratos Wistar Cyclophosphamide is a cytostatic agent used in conditioning regimens for bone marrow transplantation and in some solid tumors. It has the capacity to induce alterations in several organ systems, particularly the stomatognathic system. Previous studies by our team demonstrated an oxidative and functional effect of this drug, assessed through increased superoxide dismutase, uric acid, and glycogen levels in rat submandibular gland. On the other hand, melatonin is known for its multipurpose action due to its capacity to attenuate structural and functional alterations in several organs with high functional activity and mitotic rates. In the present study, we evaluated histological changes in ducts and acini of the submandibular gland of rats treated with cyclophosphamide, as well as the protective effect of exogenously administered melatonin. Three-month-old adult male Wistar rats with body weight ranging from 300 to 350 grams, were used and divided into five experimental groups: 1) C: Control, 2) Control and Ethanol, 3) Cyclophosphamide, 4) Cyclophosphamide and Melatonin, and 5) Melatonin. Histological sections of submandibular glands were prepared and stained with Hematoxylin/Eosin, where possible structural changes in ducts and acini were analyzed. Glands from animals treated with cyclophosphamide showed acinar cells with macrokaryosis and hyperchromasia, binucleation with irregular contours, prominent nucleoli, and perinuclear chromatin clumps compared to control groups. It is inferred that melatonin administration at the tested dose could attenuate the structural alterations of the submandibular gland treated with this chemotherapeutic agent. The reversal of such changes would explain the reduction of antioxidant and functional effects at the glandular level described in our laboratory. Aquí tienes los tres resúmenes ajustados con formato ancho (un solo párrafo continuo por idioma), eliminando los saltos de línea y guiones de división de palabras para que el sistema de OJS los procese correctamente. Resumen (Español) Ciclofosfamida es un citostático utilizado en esquemas de acondicionamiento para trasplante de médula ósea y en algunos tumores sólidos. Tiene capacidad para provocar alteraciones en varios sistemas orgánicos entre los que se destaca el sistema estomatognático. Trabajos previos de nuestro equipo demostraron un efecto oxidativo y funcional de esta droga evaluado a través del incremento de superóxido dismutasa, ácido úrico y niveles de glucógeno en glándula submandibular de rata. Por otro lado, se conoce la acción de melatonina como agente multipropósito por su capacidad de atenuar alteraciones en la estructura y función de varios órganos con alta tasa de actividad funcional y mitosis. En el presente trabajo evaluamos cambios histológicos en conductos y acinos de glándula submandibular de ratas tratadas con ciclofosfamida, así como el efecto protector de melatonina administrada por vía exógena. Se utilizaron ratas macho Wistar adultas de tres meses de edad con peso corporal de 300 a 350 gramos, divididas en cinco grupos experimentales: 1) C: Control, 2) Control y Etanol, 3) Ciclofosfamida, 4) Ciclofosfamida y Melatonina y 5) Melatonina. Se realizaron cortes histológicos de glándulas submandibulares y tinción con Hematoxilina/Eosina, donde se analizaron posibles cambios estructurales en conductos y acinos. Las glándulas de los animales tratados con ciclofosfamida mostraron células acinares con macrocariosis e hipercromasia, binucleación con contornos irregulares, nucléolo bien evidente y grumos de cromatina perinuclear, respecto de los grupos controles. Se infiere que la administración de melatonina en la dosis ensayada podría atenuar las alteraciones estructurales de la glándula submandibular tratada con este agente quimioterapéutico. La reversión de tales cambios explicaría la reducción de efectos antioxidantes y funcionales a nivel glandular descriptos en nuestro laboratorio. A ciclofosfamida é um citostático utilizado em esquemas de condicionamento para transplante de medula óssea e em alguns tumores sólidos. Tem capacidade de provocar alterações em vários sistemas orgânicos, destacando-se o sistema estomatognático. Estudos prévios de nosso grupo demonstraram um efeito oxidativo e funcional desse fármaco, avaliado pelo aumento da superóxido dismutase, do ácido úrico e dos níveis de glicogênio na glândula submandibular de ratos. Por outro lado, a ação da melatonina é conhecida como agente multipropósito por sua capacidade de atenuar alterações na estrutura e função de vários órgãos com alta taxa de atividade funcional e mitose. No presente estudo, avaliamos alterações histológicas nos ductos e ácinos da glândula submandibular de ratos tratados com ciclofosfamida, bem como o efeito protetor da melatonina administrada por via exógena. Utilizaram-se ratos Wistar machos adultos com três meses de idade e peso corporal de 300 a 350 gramas, divididos em cinco grupos experimentais: 1) C: Controle, 2) Controle e Etanol, 3) Ciclofosfamida; 4) Ciclofosfamida e Melatonina e 5) Melatonina. Foram realizados cortes histológicos das glândulas submandibulares com coloração Hematoxilina/Eosina, analisando-se possíveis alterações estruturais em ductos e ácinos. As glândulas dos animais tratados com ciclofosfamida apresentaram células acinares com macrocariose e hipercromasia, binucleação com contornos irregulares, nucléolo proeminente e grumos de cromatina perinuclear, quando comparadas aos grupos controle. Infere-se que a administração de melatonina na dose testada poderia atenuar as alterações estruturais da glândula submandibular tratada com este agente quimioterapêutico. A reversão de tais alterações explicaria a redução dos efeitos antioxidantes e funcionais em nível glandular descritos por nosso laboratório. Facultad de Odontología de la Universidad Nacional del Nordeste (FOUNNE) 2025-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/rfo/article/view/9030 10.30972/rfo.1829030 Revista de la Facultad de Odontología; Vol. 18 Núm. 2 (2025) 2683-7986 1668-7280 spa https://revistas.unne.edu.ar/index.php/rfo/article/view/9030/8765 https://creativecommons.org/licenses/by-nc/4.0

Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide

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