Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina
Ehrlichiosis is a vector-borne disease that affects numerous species. It is caused by Ehrlichia spp. gram-negative, obligate intracellular bacteria with a coccoid or pleomorphic appearance that infects monocytes. Diagnosis, through clinical symptoms, is difficult given the similarity of presentation...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2023
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/7049 |
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I48-R154-article-70492023-11-28T12:58:53Z Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina Alteraciones hematológicas en perros (Canis lupus familiaris) diagnosticados con Ehrlichia spp. por PCR, en clínicas veterinarias del Nordeste Argentino Mansilla, S.L Delgado, M.B. Rossner, M. Victoria Cainzos, Romina P. Merino, L.A. Koscinczuk, P. Rhipicephalus sanguineus sensu lato hematologic diagnosis domestic canines Rhipicephalus sanguineus sensu lato diagnóstico hematológico caninos domésticos Ehrlichiosis is a vector-borne disease that affects numerous species. It is caused by Ehrlichia spp. gram-negative, obligate intracellular bacteria with a coccoid or pleomorphic appearance that infects monocytes. Diagnosis, through clinical symptoms, is difficult given the similarity of presentation with other vector-borne disease. The evaluation of hematological parameters (hemogram and blood smear) is the first indication of the clinical veterinarian, since it is possible to find intracytoplasmic inclusions (morulae) in monocytes. The aim of this study was to evaluate hematological alterations in dogs diagnosed with Ehrlichia spp. by peripheral blood smear, and to confirm it by molecular technique (PCR). From June/2021 to July/2022 dog samples were analyzed and intracytoplasmic inclusions were identified in seven samples. The presence of anemia and thrombocytopenia without alterations in the number of leukocytes was observed in 6 of 7 blood samples. The PCR technique confirmed the initial diagnosis in 2/7 samples, amplifying the dsb 330/728 gene segment. The usefulness of hematological parameters together with the diagnostic confirmation by PCR technique is emphasized, the latter being a highly sensitive and specific method, which offers up to 100% diagnostic confidence in detecting and amplifying the DNA of Ehrlichia spp. La ehrlichiosis es una enfermedad transmitida por vectores (ETV) que afecta a numerosas especies. Es causada por Ehrlichia spp. una bacteria gramnegativa, intracelular obligada, de aspecto cocoide o pleomórfica, que infecta los monocitos. El diagnóstico, a través de los síntomas clínicos, se dificulta dada la similitud de presentación con otras ETV. La evaluación de los parámetros hematológicos (hemograma y extendido sanguíneo) es la primera indicación del veterinario clínico, ya que es posible encontrar inclusiones intracitoplasmáticas (mórulas) en los monocitos. El objetivo del trabajo fue evaluar las alteraciones hematológicas de perros diagnosticados con Ehrlichia spp. por extendido de sangre periférica, y confirmarlo por técnica molecular (PCR). De junio/2021 a julio/2022 se analizaron muestras de perros identificando inclusiones intracitoplasmáticas en siete muestras. Se observó en 6 de 7 muestras de sangre la presencia de anemia y trombocitopenia sin alteraciones del número de leucocitos. La técnica de PCR confirmo el diagnóstico inicial en 2/7 muestras, amplificando el segmento del gen dsb 330/728. Se destaca la utilidad de los parámetros hematológicos junto a la confirmación diagnóstica por la técnica de PCR, siendo esta última, un método altamente sensible y específico, que ofrece hasta un 100% de seguridad en el diagnóstico detectando y amplificando el ADN de Ehrlichia spp. Universidad Nacional del Nordeste 2023-11-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7049 10.30972/vet.3427049 Revista Veterinaria; Vol. 34 Núm. 2 (2023); 91-95 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7049/6505 https://creativecommons.org/licenses/by/2.5/ar/ |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Rhipicephalus sanguineus sensu lato hematologic diagnosis domestic canines Rhipicephalus sanguineus sensu lato diagnóstico hematológico caninos domésticos |
| spellingShingle |
Rhipicephalus sanguineus sensu lato hematologic diagnosis domestic canines Rhipicephalus sanguineus sensu lato diagnóstico hematológico caninos domésticos Mansilla, S.L Delgado, M.B. Rossner, M. Victoria Cainzos, Romina P. Merino, L.A. Koscinczuk, P. Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina |
| topic_facet |
Rhipicephalus sanguineus sensu lato hematologic diagnosis domestic canines Rhipicephalus sanguineus sensu lato diagnóstico hematológico caninos domésticos |
| author |
Mansilla, S.L Delgado, M.B. Rossner, M. Victoria Cainzos, Romina P. Merino, L.A. Koscinczuk, P. |
| author_facet |
Mansilla, S.L Delgado, M.B. Rossner, M. Victoria Cainzos, Romina P. Merino, L.A. Koscinczuk, P. |
| author_sort |
Mansilla, S.L |
| title |
Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina |
| title_short |
Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina |
| title_full |
Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina |
| title_fullStr |
Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina |
| title_full_unstemmed |
Hematological alterations in dogs (Canis lupus familiaris) diagnosed with Ehrlichia spp. by PCR in veterinary clinics in Northeastern Argentina |
| title_sort |
hematological alterations in dogs (canis lupus familiaris) diagnosed with ehrlichia spp. by pcr in veterinary clinics in northeastern argentina |
| description |
Ehrlichiosis is a vector-borne disease that affects numerous species. It is caused by Ehrlichia spp. gram-negative, obligate intracellular bacteria with a coccoid or pleomorphic appearance that infects monocytes. Diagnosis, through clinical symptoms, is difficult given the similarity of presentation with other vector-borne disease. The evaluation of hematological parameters (hemogram and blood smear) is the first indication of the clinical veterinarian, since it is possible to find intracytoplasmic inclusions (morulae) in monocytes. The aim of this study was to evaluate hematological alterations in dogs diagnosed with Ehrlichia spp. by peripheral blood smear, and to confirm it by molecular technique (PCR). From June/2021 to July/2022 dog samples were analyzed and intracytoplasmic inclusions were identified in seven samples. The presence of anemia and thrombocytopenia without alterations in the number of leukocytes was observed in 6 of 7 blood samples. The PCR technique confirmed the initial diagnosis in 2/7 samples, amplifying the dsb 330/728 gene segment. The usefulness of hematological parameters together with the diagnostic confirmation by PCR technique is emphasized, the latter being a highly sensitive and specific method, which offers up to 100% diagnostic confidence in detecting and amplifying the DNA of Ehrlichia spp. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2023 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/7049 |
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