Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues

The polyunsaturated fatty acid composition, chemiluminescence and peroxidizability indexof mitochondria obtained from rat liver, kidney, lung and heart, were studied after oral administrationof conjugated linoleic acid (CLA). After incubation of mitochondria in an ascorbateFe++ system (120 min at 37...

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Detalles Bibliográficos
Autores principales: Palacios, A., Piergiacomi, V. A.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2005
Materias:
Conjugated linoleic acid
mitochondria
peroxidation
chemiluminescence.
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/1983
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-1983
record_format ojs
spelling I48-R154-article-19832025-02-12T21:58:28Z Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues Palacios, A. Piergiacomi, V. A. Conjugated linoleic acid mitochondria peroxidation chemiluminescence. The polyunsaturated fatty acid composition, chemiluminescence and peroxidizability indexof mitochondria obtained from rat liver, kidney, lung and heart, were studied after oral administrationof conjugated linoleic acid (CLA). After incubation of mitochondria in an ascorbateFe++ system (120 min at 37°C), it was observed that the total counts per min/mg protein originatedfrom light emission: chemiluminescence, was lower in liver and kidney mitochondriain the CLA group than in the control group. When peroxidized mitochondrias were comparedto natives, in CLA and control groups it was observed that 18:2 n–6 decreased in all tissuesused in this work. In the control group, it also decrease C20:4 n–6 and C22:6 n–6 in kidneyand liver, while in lung it was affected C22:6 n–3. In CLA group, C20:4 n–6 decreased inliver and kidney, whereas in liver it also decreased C22:6 n–3. As a consequence, the peroxidizabilityindex –a parameter based on the maximal rate of oxidation of fatty acids– showedsignificant changes in liver and kidney mitochondria. These changes were less pronounced inmembranes derived from rats receiving CLA per os. Our results confirm and extend previousobservations that indicated that CLA may act as an antioxidant, protecting membranes fromdeleterious effects. Universidad Nacional del Nordeste 2005-02-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/1983 10.30972/vet.1611983 Revista Veterinaria; Vol. 16 Núm. 1 (2005); 3-8 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/1983/1730 Derechos de autor 2005 A. Palacios, V. A. Piergiacomi https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Conjugated linoleic acid
mitochondria
peroxidation
chemiluminescence.
spellingShingle Conjugated linoleic acid
mitochondria
peroxidation
chemiluminescence.
Palacios, A.
Piergiacomi, V. A.
Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
topic_facet Conjugated linoleic acid
mitochondria
peroxidation
chemiluminescence.
author Palacios, A.
Piergiacomi, V. A.
author_facet Palacios, A.
Piergiacomi, V. A.
author_sort Palacios, A.
title Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
title_short Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
title_full Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
title_fullStr Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
title_full_unstemmed Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
title_sort effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
description The polyunsaturated fatty acid composition, chemiluminescence and peroxidizability indexof mitochondria obtained from rat liver, kidney, lung and heart, were studied after oral administrationof conjugated linoleic acid (CLA). After incubation of mitochondria in an ascorbateFe++ system (120 min at 37°C), it was observed that the total counts per min/mg protein originatedfrom light emission: chemiluminescence, was lower in liver and kidney mitochondriain the CLA group than in the control group. When peroxidized mitochondrias were comparedto natives, in CLA and control groups it was observed that 18:2 n–6 decreased in all tissuesused in this work. In the control group, it also decrease C20:4 n–6 and C22:6 n–6 in kidneyand liver, while in lung it was affected C22:6 n–3. In CLA group, C20:4 n–6 decreased inliver and kidney, whereas in liver it also decreased C22:6 n–3. As a consequence, the peroxidizabilityindex –a parameter based on the maximal rate of oxidation of fatty acids– showedsignificant changes in liver and kidney mitochondria. These changes were less pronounced inmembranes derived from rats receiving CLA per os. Our results confirm and extend previousobservations that indicated that CLA may act as an antioxidant, protecting membranes fromdeleterious effects.
publisher Universidad Nacional del Nordeste
publishDate 2005
url https://revistas.unne.edu.ar/index.php/vet/article/view/1983
work_keys_str_mv AT palaciosa effectofconjugatedlinoleicacidonnonenzymaticlipidperoxidationofmitochondriaobtainedfromdifferentrattissues
AT piergiacomiva effectofconjugatedlinoleicacidonnonenzymaticlipidperoxidationofmitochondriaobtainedfromdifferentrattissues
first_indexed 2025-05-17T05:09:12Z
last_indexed 2025-05-17T05:09:12Z
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