Lipid peroxidation assays in canine fresh semen
Lipid peroxidation (LP) is a potential cause of infertility in males of numerous species. Levels in which the spermatozoa loses mobility in vitro is correlated with the rate of LP that it suffers. The objective of this work was to determine the fatty acid composition and analyze the sensitivity to L...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2009
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/1857 |
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I48-R154-article-18572025-02-12T21:57:53Z Lipid peroxidation assays in canine fresh semen Gavazza, M. Gutiérrez, A. M. Marmunti, M. Palacios, A. dog spermatozoa lipid peroxidation polyunsaturated fatty acids. Lipid peroxidation (LP) is a potential cause of infertility in males of numerous species. Levels in which the spermatozoa loses mobility in vitro is correlated with the rate of LP that it suffers. The objective of this work was to determine the fatty acid composition and analyze the sensitivity to LP (ascorbate–Fe++ dependent) in spermatozoa obtained from different samples of canine fresh semen. LP was evaluated using chemiluminescence (CL) (cpm/mg of protein) and fatty acid (FA) profile by means of gas chromatography. The saturated FA content found in the analyzed spermatozoa was approximately 40%, whereas the total unsaturated FA content was approximately 55% with a prevalence of docosapentaenoic acid (C22:5 n6). When the control and ascorbate–Fe++ dependent samples were compared, it was observed a significant increase in the light emission (CL). Consequently, significant decrease in the percentage of the polyunsaturated fatty acids (PUFA) was obtained, being more affected: C18: 2 n6, C20: 4 n6, C22: 4 n6, C22: 5 n6 and C22: 6 n3. The unsaturation index used to evaluate the alterations generated during the LP was correlated with the data mentioned before. Our results indicate that dog semen contains great amounts of PUFA, which were vulnerable to the LP. The alteration in the PUFA composition could be the common base of different degenerative processes. Universidad Nacional del Nordeste 2009-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/1857 10.30972/vet.2021857 Revista Veterinaria; Vol. 20 Núm. 2 (2009); 97-102 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/1857/1607 Derechos de autor 2009 M. Gavazza, A. M. Gutiérrez, M. Marmunti, A. Palacios https://creativecommons.org/licenses/by-nc/4.0 |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
dog spermatozoa lipid peroxidation polyunsaturated fatty acids. |
| spellingShingle |
dog spermatozoa lipid peroxidation polyunsaturated fatty acids. Gavazza, M. Gutiérrez, A. M. Marmunti, M. Palacios, A. Lipid peroxidation assays in canine fresh semen |
| topic_facet |
dog spermatozoa lipid peroxidation polyunsaturated fatty acids. |
| author |
Gavazza, M. Gutiérrez, A. M. Marmunti, M. Palacios, A. |
| author_facet |
Gavazza, M. Gutiérrez, A. M. Marmunti, M. Palacios, A. |
| author_sort |
Gavazza, M. |
| title |
Lipid peroxidation assays in canine fresh semen |
| title_short |
Lipid peroxidation assays in canine fresh semen |
| title_full |
Lipid peroxidation assays in canine fresh semen |
| title_fullStr |
Lipid peroxidation assays in canine fresh semen |
| title_full_unstemmed |
Lipid peroxidation assays in canine fresh semen |
| title_sort |
lipid peroxidation assays in canine fresh semen |
| description |
Lipid peroxidation (LP) is a potential cause of infertility in males of numerous species. Levels in which the spermatozoa loses mobility in vitro is correlated with the rate of LP that it suffers. The objective of this work was to determine the fatty acid composition and analyze the sensitivity to LP (ascorbate–Fe++ dependent) in spermatozoa obtained from different samples of canine fresh semen. LP was evaluated using chemiluminescence (CL) (cpm/mg of protein) and fatty acid (FA) profile by means of gas chromatography. The saturated FA content found in the analyzed spermatozoa was approximately 40%, whereas the total unsaturated FA content was approximately 55% with a prevalence of docosapentaenoic acid (C22:5 n6). When the control and ascorbate–Fe++ dependent samples were compared, it was observed a significant increase in the light emission (CL). Consequently, significant decrease in the percentage of the polyunsaturated fatty acids (PUFA) was obtained, being more affected: C18: 2 n6, C20: 4 n6, C22: 4 n6, C22: 5 n6 and C22: 6 n3. The unsaturation index used to evaluate the alterations generated during the LP was correlated with the data mentioned before. Our results indicate that dog semen contains great amounts of PUFA, which were vulnerable to the LP. The alteration in the PUFA composition could be the common base of different degenerative processes. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2009 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/1857 |
| work_keys_str_mv |
AT gavazzam lipidperoxidationassaysincaninefreshsemen AT gutierrezam lipidperoxidationassaysincaninefreshsemen AT marmuntim lipidperoxidationassaysincaninefreshsemen AT palaciosa lipidperoxidationassaysincaninefreshsemen |
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2025-05-17T05:08:32Z |
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2025-05-17T05:08:32Z |
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1832343057229414400 |