Proteomic Analysis of Climatic Keratopathy Droplets

PURPOSE. To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS. Proteomic mass spectrometry of the CDK specimens was perfor...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Menegay, Michelle, DeMia, Lee, Tabbara, Khalid F., Cafaro, Thamara A., Urrets Zavalía, Julio, Serra, Horacio M., Bhattacharya, Sanjoy K.
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: 2008
Materias:
RE Oftalmología
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4806/1/A_Menegay.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4806
record_format dspace
spelling I38-R144-48062025-04-28T16:25:27Z http://pa.bibdigital.ucc.edu.ar/4806/ Proteomic Analysis of Climatic Keratopathy Droplets Menegay, Michelle DeMia, Lee Tabbara, Khalid F. Cafaro, Thamara A. Urrets Zavalía, Julio Serra, Horacio M. Bhattacharya, Sanjoy K. RE Oftalmología PURPOSE. To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS. Proteomic mass spectrometry of the CDK specimens was performed after fractionation of proteins in 4% to 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies was performed to confirm the presence of identified proteins on donor tissues from patients with CDK and control subjects. RESULTS. Proteomic analyses revealed identification of 105 proteins in CDK specimens. Immunohistochemical analyses confirmed localization of annexin A2 and glyceraldehyde 3-dehydrogenase (GAPDH), proteins identified by proteomic analyses in CDK specimens. The proteins were subjected to analyses with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Database which showed that a few biochemical pathways were more frequent for the identified proteins. CONCLUSIONS. Approximately 105 proteins were identified in CDK specimens, and a subset of them was confirmed by immunohistochemistry. Several of these may play a role in fibril or deposit formation. 2008-12-01 application/pdf spa http://pa.bibdigital.ucc.edu.ar/4806/1/A_Menegay.pdf Menegay, Michelle, DeMia, Lee, Tabbara, Khalid F., Cafaro, Thamara A., Urrets Zavalía, Julio ORCID: https://orcid.org/0000-0003-2267-2783 <https://orcid.org/0000-0003-2267-2783>, Serra, Horacio M. and Bhattacharya, Sanjoy K. (2008) Proteomic Analysis of Climatic Keratopathy Droplets. Investigative Ophthalmology & Visual Science, 49 (7). ISSN 2829-2837 https://iovs.arvojournals.org/article.aspx?articleid=2125493 info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1167/iovs.07-1438 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion Fil: Menegay, Michelle. Bascom Palmer Eye Institute, University of Miami, Miami, Florida Fil: DeMia, Lee. Bascom Palmer Eye Institute, University of Miami, Miami, Florida Fil: Tabbara, Khalid F. The Eye Center and The Eye Foundation for Research in Ophthalmology, Riyadh, Saudi Arabia Fil: Cafaro, Thamara A. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba Fil: Urrets Zavalía, Julio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina Fil: Serra, Horacio M. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba Fil: Bhattacharya, Sanjoy K. Bascom Palmer Eye Institute, University of Miami, Miami, Florida info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic RE Oftalmología
spellingShingle RE Oftalmología
Menegay, Michelle
DeMia, Lee
Tabbara, Khalid F.
Cafaro, Thamara A.
Urrets Zavalía, Julio
Serra, Horacio M.
Bhattacharya, Sanjoy K.
Proteomic Analysis of Climatic Keratopathy Droplets
topic_facet RE Oftalmología
description PURPOSE. To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS. Proteomic mass spectrometry of the CDK specimens was performed after fractionation of proteins in 4% to 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies was performed to confirm the presence of identified proteins on donor tissues from patients with CDK and control subjects. RESULTS. Proteomic analyses revealed identification of 105 proteins in CDK specimens. Immunohistochemical analyses confirmed localization of annexin A2 and glyceraldehyde 3-dehydrogenase (GAPDH), proteins identified by proteomic analyses in CDK specimens. The proteins were subjected to analyses with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Database which showed that a few biochemical pathways were more frequent for the identified proteins. CONCLUSIONS. Approximately 105 proteins were identified in CDK specimens, and a subset of them was confirmed by immunohistochemistry. Several of these may play a role in fibril or deposit formation.
format Artículo
Artículo
acceptedVersion
author Menegay, Michelle
DeMia, Lee
Tabbara, Khalid F.
Cafaro, Thamara A.
Urrets Zavalía, Julio
Serra, Horacio M.
Bhattacharya, Sanjoy K.
author_facet Menegay, Michelle
DeMia, Lee
Tabbara, Khalid F.
Cafaro, Thamara A.
Urrets Zavalía, Julio
Serra, Horacio M.
Bhattacharya, Sanjoy K.
author_sort Menegay, Michelle
title Proteomic Analysis of Climatic Keratopathy Droplets
title_short Proteomic Analysis of Climatic Keratopathy Droplets
title_full Proteomic Analysis of Climatic Keratopathy Droplets
title_fullStr Proteomic Analysis of Climatic Keratopathy Droplets
title_full_unstemmed Proteomic Analysis of Climatic Keratopathy Droplets
title_sort proteomic analysis of climatic keratopathy droplets
publishDate 2008
url http://pa.bibdigital.ucc.edu.ar/4806/1/A_Menegay.pdf
work_keys_str_mv AT menegaymichelle proteomicanalysisofclimatickeratopathydroplets
AT demialee proteomicanalysisofclimatickeratopathydroplets
AT tabbarakhalidf proteomicanalysisofclimatickeratopathydroplets
AT cafarothamaraa proteomicanalysisofclimatickeratopathydroplets
AT urretszavaliajulio proteomicanalysisofclimatickeratopathydroplets
AT serrahoraciom proteomicanalysisofclimatickeratopathydroplets
AT bhattacharyasanjoyk proteomicanalysisofclimatickeratopathydroplets
_version_ 1832592662024159232

Ejemplares similares