Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut

The most potent peanut allergens, Ara h 2 and Ara h 6, were silenced in transgenic plants by RNA interference. Three independent transgenic lines were recovered after microprojectile bombardment, of which two contained single, integrated copies of the transgene. The third line contained multiple cop...

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Detalles Bibliográficos
Autores principales: Chu, Ye, Faustinelli, Paola Carmen, Ramos, María Laura, Hajduch, Martin, Stevenson, Severin, Thelen, Jay J., Maleki, Soheila J., Cheng, Hsiaopo, Ozias Akins, Peggy
Formato: Artículo
Lenguaje:Español
Publicado: 2008
Materias:
SB Cultura de la planta
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4178/1/A_Chu_Faustinelli.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4178
record_format dspace
spelling I38-R144-41782025-04-08T21:39:39Z http://pa.bibdigital.ucc.edu.ar/4178/ Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut Chu, Ye Faustinelli, Paola Carmen Ramos, María Laura Hajduch, Martin Stevenson, Severin Thelen, Jay J. Maleki, Soheila J. Cheng, Hsiaopo Ozias Akins, Peggy SB Cultura de la planta The most potent peanut allergens, Ara h 2 and Ara h 6, were silenced in transgenic plants by RNA interference. Three independent transgenic lines were recovered after microprojectile bombardment, of which two contained single, integrated copies of the transgene. The third line contained multiple copies of the transgene. Ara h 2 expression was significantly suppressed in all three lines, whereas Ara h 6 was reduced in two lines. Expression of peanut allergens Ara h 1 and Ara h 3 was not noticeably affected. Significant reduction of human IgE binding to Ara h 2 and Ara h 6 also was observed. Seed weight and germination data from transgenic and nontransgenic segregants showed no significant differences. Data collected from in vitro Aspergillus flavus infection indicate no significant difference in fungal growth between the transgenic lines and the nontransgenic controls. These data suggest that silencing Ara h 2 and Ara h 6 is a feasible approach to produce hypoallergenic peanut. 2008-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/4178/1/A_Chu_Faustinelli.pdf Chu, Ye, Faustinelli, Paola Carmen ORCID: https://orcid.org/0000-0002-6925-4610 <https://orcid.org/0000-0002-6925-4610>, Ramos, María Laura, Hajduch, Martin, Stevenson, Severin, Thelen, Jay J., Maleki, Soheila J., Cheng, Hsiaopo and Ozias Akins, Peggy (2008) Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut. Journal of Agricultural and Food Chemistry, 56 (23). pp. 11225-11233. ISSN 0021-8561 info:eu-repo/semantics/altIdentifier/doi/10.1021/jf802600r
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic SB Cultura de la planta
spellingShingle SB Cultura de la planta
Chu, Ye
Faustinelli, Paola Carmen
Ramos, María Laura
Hajduch, Martin
Stevenson, Severin
Thelen, Jay J.
Maleki, Soheila J.
Cheng, Hsiaopo
Ozias Akins, Peggy
Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut
topic_facet SB Cultura de la planta
description The most potent peanut allergens, Ara h 2 and Ara h 6, were silenced in transgenic plants by RNA interference. Three independent transgenic lines were recovered after microprojectile bombardment, of which two contained single, integrated copies of the transgene. The third line contained multiple copies of the transgene. Ara h 2 expression was significantly suppressed in all three lines, whereas Ara h 6 was reduced in two lines. Expression of peanut allergens Ara h 1 and Ara h 3 was not noticeably affected. Significant reduction of human IgE binding to Ara h 2 and Ara h 6 also was observed. Seed weight and germination data from transgenic and nontransgenic segregants showed no significant differences. Data collected from in vitro Aspergillus flavus infection indicate no significant difference in fungal growth between the transgenic lines and the nontransgenic controls. These data suggest that silencing Ara h 2 and Ara h 6 is a feasible approach to produce hypoallergenic peanut.
format Artículo
author Chu, Ye
Faustinelli, Paola Carmen
Ramos, María Laura
Hajduch, Martin
Stevenson, Severin
Thelen, Jay J.
Maleki, Soheila J.
Cheng, Hsiaopo
Ozias Akins, Peggy
author_facet Chu, Ye
Faustinelli, Paola Carmen
Ramos, María Laura
Hajduch, Martin
Stevenson, Severin
Thelen, Jay J.
Maleki, Soheila J.
Cheng, Hsiaopo
Ozias Akins, Peggy
author_sort Chu, Ye
title Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut
title_short Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut
title_full Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut
title_fullStr Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut
title_full_unstemmed Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut
title_sort reduction of ige binding and nonpromotion of aspergillus flavus fungal growth by simultaneously silencing ara h 2 and ara h 6 in peanut
publishDate 2008
url http://pa.bibdigital.ucc.edu.ar/4178/1/A_Chu_Faustinelli.pdf
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