Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis

Purpose. To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune reaction in a rat model of Staphylococcus aureus endophthalmitis. Methods. Seventy-five female Lewis rats were divided into...

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Autores principales: Reviglio, Victor Eduardo, Sambuelli, Rubén Horacio, Olmedo, Alejandra, Falco, Micaela, Echenique, José, O'Brien, Terrence P., Kuo, Irene C.
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: 2007
Materias:
RE Oftalmología
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4011/1/A_Reviglio_Sambuelli_Olmedo_Falco_Echenique_O%27Brien_Kuo.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4011
record_format dspace
spelling I38-R144-40112024-02-28T16:21:01Z http://pa.bibdigital.ucc.edu.ar/4011/ Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis Reviglio, Victor Eduardo Sambuelli, Rubén Horacio Olmedo, Alejandra Falco, Micaela Echenique, José O'Brien, Terrence P. Kuo, Irene C. RE Oftalmología Purpose. To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune reaction in a rat model of Staphylococcus aureus endophthalmitis. Methods. Seventy-five female Lewis rats were divided into three groups: the endophthalmitis group received an intravitreal injection of 65 colony-forming units of viable S. aureus, the vehicle-injected group received balanced sterile saline solution (BSS), and the control group was not injected. Eyes were enucleated at 24 and 48 hours and processed for immunohistochemical staining and Western blot studies for SLPI. Results. In S. aureus endophthalmitis eyes, there was strong immunostaining for SLPI in the retina and vitreous with associated neutrophilic infiltrates. At 48 hours, corneas also stained for SLPI. Western blots confirmed increased SLPI expression in all infected eyes. By immunohistochemical assays, SLPI was absent in the BSS and control eyes. The causative pathogen was identified in all samples from the endophthalmitis group by traditional culture methods. Conclusions. To our knowledge, this report is the first to demonstrate the presence of SLPI in the inflamed cornea, vitreous, and retina tissues of rat eyes with S. aureus endophthalmitis, suggesting that SLPI has an active role in the innate immunity of the eye. Release of SLPI by inflammatory cells in the anterior and posterior segments may contribute to the host defense response against infectious endophthalmitis. 2007-12-31 application/pdf spa http://pa.bibdigital.ucc.edu.ar/4011/1/A_Reviglio_Sambuelli_Olmedo_Falco_Echenique_O%27Brien_Kuo.pdf Reviglio, Victor Eduardo ORCID: https://orcid.org/0000-0002-4368-5033 <https://orcid.org/0000-0002-4368-5033>, Sambuelli, Rubén Horacio, Olmedo, Alejandra, Falco, Micaela, Echenique, José, O'Brien, Terrence P. and Kuo, Irene C. ORCID: https://orcid.org/0000-0001-9350-5478 <https://orcid.org/0000-0001-9350-5478> (2007) Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis. Mediators of Inflammation, 2007. ISSN 1466-1861 info:eu-repo/semantics/altIdentifier/doi/10.1155/2007/93857 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion Fil: Reviglio, Víctor Eduardo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina Fil: Sambuelli, Rubén Horacio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina Fil: Olmedo, Alejandra. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina Fil: Falco, Micaela. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina Fil: Echenique, José. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina Fil: O'Brien, Terrence P. Cornea and External Diseases, School of Medicine, University of Miami, Miami, FL 33136, United States Fil: Kuo, Irene C. Cornea Department, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, United States info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic RE Oftalmología
spellingShingle RE Oftalmología
Reviglio, Victor Eduardo
Sambuelli, Rubén Horacio
Olmedo, Alejandra
Falco, Micaela
Echenique, José
O'Brien, Terrence P.
Kuo, Irene C.
Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis
topic_facet RE Oftalmología
description Purpose. To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune reaction in a rat model of Staphylococcus aureus endophthalmitis. Methods. Seventy-five female Lewis rats were divided into three groups: the endophthalmitis group received an intravitreal injection of 65 colony-forming units of viable S. aureus, the vehicle-injected group received balanced sterile saline solution (BSS), and the control group was not injected. Eyes were enucleated at 24 and 48 hours and processed for immunohistochemical staining and Western blot studies for SLPI. Results. In S. aureus endophthalmitis eyes, there was strong immunostaining for SLPI in the retina and vitreous with associated neutrophilic infiltrates. At 48 hours, corneas also stained for SLPI. Western blots confirmed increased SLPI expression in all infected eyes. By immunohistochemical assays, SLPI was absent in the BSS and control eyes. The causative pathogen was identified in all samples from the endophthalmitis group by traditional culture methods. Conclusions. To our knowledge, this report is the first to demonstrate the presence of SLPI in the inflamed cornea, vitreous, and retina tissues of rat eyes with S. aureus endophthalmitis, suggesting that SLPI has an active role in the innate immunity of the eye. Release of SLPI by inflammatory cells in the anterior and posterior segments may contribute to the host defense response against infectious endophthalmitis.
format Artículo
Artículo
acceptedVersion
author Reviglio, Victor Eduardo
Sambuelli, Rubén Horacio
Olmedo, Alejandra
Falco, Micaela
Echenique, José
O'Brien, Terrence P.
Kuo, Irene C.
author_facet Reviglio, Victor Eduardo
Sambuelli, Rubén Horacio
Olmedo, Alejandra
Falco, Micaela
Echenique, José
O'Brien, Terrence P.
Kuo, Irene C.
author_sort Reviglio, Victor Eduardo
title Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis
title_short Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis
title_full Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis
title_fullStr Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis
title_full_unstemmed Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis
title_sort secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in staphylococcus aureus endophthalmitis
publishDate 2007
url http://pa.bibdigital.ucc.edu.ar/4011/1/A_Reviglio_Sambuelli_Olmedo_Falco_Echenique_O%27Brien_Kuo.pdf
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