Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments

Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed th...

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Autores principales: Figueroa, Ana Cristina, Díaz, María Soledad, Alasino, Roxana Valeria, Yang, Pablo, Soria, Néstor Walter, Beltramo, Dante Miguel
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: 2022
Materias:
QD Química
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3681/1/A_Figueroa_D%C3%ADaz_Alasino_Yang_Soria_Beltramo.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3681
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
spellingShingle QD Química
Figueroa, Ana Cristina
Díaz, María Soledad
Alasino, Roxana Valeria
Yang, Pablo
Soria, Néstor Walter
Beltramo, Dante Miguel
Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
topic_facet QD Química
description Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed the presence and location of α-tubulin isotypes and enzymes that participate in tyrosination-detyrosination in the three stages of T. frezii. Although both tyrosinated and detyrosinated tubulin seem to be associated with a membrane fraction component that gives it a similar behavior to integral proteins, in the soluble cytosolic fraction, only detyrosinated tubulin was detected, not tyrosinated tubulin. The presence of α-tubulin was not detected using the monoclonal antibody DM1A as neither acetylated tubulin. The RNA-Seq analysis showed the presence of α, β, and γ-tubulins and the genes that codes for tyrosine-tubulin ligase and cytosolic carboxypeptidase 1, enzymes that are involved in post-translational modification processes. These sequences showed a high percentage of identity and homology with Ustilago maydis, Thecaphora thlaspeos, and Anthracocystis flocculosa. This is the first report for tubulins subpopulations and the cellular distribution in T. frezii, which together with the data obtained by RNA-Seq contribute to the knowledge of the pathogen, which will allow the development of control strategies. © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.
format Artículo
Artículo
acceptedVersion
author Figueroa, Ana Cristina
Díaz, María Soledad
Alasino, Roxana Valeria
Yang, Pablo
Soria, Néstor Walter
Beltramo, Dante Miguel
author_facet Figueroa, Ana Cristina
Díaz, María Soledad
Alasino, Roxana Valeria
Yang, Pablo
Soria, Néstor Walter
Beltramo, Dante Miguel
author_sort Figueroa, Ana Cristina
title Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_short Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_full Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_fullStr Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_full_unstemmed Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_sort expression and localization of tubulin isotypes and its mrnas during thecaphora frezii developments
publishDate 2022
url http://pa.bibdigital.ucc.edu.ar/3681/1/A_Figueroa_D%C3%ADaz_Alasino_Yang_Soria_Beltramo.pdf
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