Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201

Salmonellosis is a common subclinical infection in pigs and therefore apparently healthy animals may represent a reservoir of antibiotic-resistant Salmonella for humans. This study estimates and characterizes resistance to two classes of antimicrobials considered of the highest priority within the c...

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Autores principales: Sevilla, Eloísa, Vico, Juan Pablo, Delgado-Blas, José F., González-Zorn, Bruno, Marín, Clara M., Uruén, Cristina, Martín-Burriel, Inmaculada, Bolea, Rosa, Mainar-Jaime, Raúl C.
Formato: Artículo
Lenguaje:Español
Publicado: Elsevier B.V. 2021
Materias:
QL Zoología
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3435/1/A_Sevilla_Vico_Delago-Blas.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3435
record_format dspace
spelling I38-R144-34352025-04-14T18:16:33Z http://pa.bibdigital.ucc.edu.ar/3435/ Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201 Sevilla, Eloísa Vico, Juan Pablo Delgado-Blas, José F. González-Zorn, Bruno Marín, Clara M. Uruén, Cristina Martín-Burriel, Inmaculada Bolea, Rosa Mainar-Jaime, Raúl C. QL Zoología Salmonellosis is a common subclinical infection in pigs and therefore apparently healthy animals may represent a reservoir of antibiotic-resistant Salmonella for humans. This study estimates and characterizes resistance to two classes of antimicrobials considered of the highest priority within the critically important antimicrobials for humans, i.e. colistin (CR) and 3rd generation cephalosporins (3GC), on a collection of Salmonella isolates from pigs from two periods: between 2008 and 09, when colistin was massively used; and in 2018, after three years under a National Plan against Antibiotic Resistance. Prevalence of CR was low (6 out of 625; 0.96%; 95%CI: 0.44–2.1) in 2008–09 and associated mostly to the mcr-1 gene, which was detected in four S. 4,5,12:i:- isolates. Polymorphisms in the pmrAB genes were detected in a S. 9,12:-:- isolate. No CR was detected in 2018 out of 59 isolates tested. Among 270 Salmonella isolates considered for the assessment of resistance to 3GC in the 2008–2009 sampling, only one Salmonella Bredeney (0.37%; 95%CI: 0.07–2.1) showed resistance to 3GC, which was associated with the blaCMY-2 gene (AmpC producer). In 2018, six isolates out of 59 (10.2%; 95%CI: 4.7–20.5) showed resistance to 3GC, but only two different strains were identified (S. 4,12:i:- and S. Rissen), both confirmed as extended-spectrum β-lactamases (ESBL) producers. The blaCTX-M-3 and blaTEM-1b genes in S. 4,12:i:- and the blaTEM-1b gene in S. Rissen seemed to be associated with this resistance. Overall, the prevalence of CR in Salmonella appeared to be very low in 2008–2009 despite the considerable use of colistin in pigs at that time, and seemed to remain so in 2018. Resistance to 3GC was even lower in 2008–2009 but somewhat higher in 2018. Resistance was mostly coded by genes associated with mobile genetic elements. Most serotypes involved in these antimicrobial resistances displayed a multidrug resistance pattern and were considered zoonotic. Elsevier B.V. 2021-01-02 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/3435/1/A_Sevilla_Vico_Delago-Blas.pdf Sevilla, Eloísa, Vico, Juan Pablo ORCID: https://orcid.org/0000-0001-5313-7033 <https://orcid.org/0000-0001-5313-7033>, Delgado-Blas, José F. ORCID: https://orcid.org/0000-0001-6447-7113 <https://orcid.org/0000-0001-6447-7113>, González-Zorn, Bruno ORCID: https://orcid.org/0000-0003-2687-9853 <https://orcid.org/0000-0003-2687-9853>, Marín, Clara M. ORCID: https://orcid.org/0000-0002-1974-9025 <https://orcid.org/0000-0002-1974-9025>, Uruén, Cristina, Martín-Burriel, Inmaculada ORCID: https://orcid.org/0000-0001-6016-4726 <https://orcid.org/0000-0001-6016-4726>, Bolea, Rosa ORCID: https://orcid.org/0000-0002-2746-3932 <https://orcid.org/0000-0002-2746-3932> and Mainar-Jaime, Raúl C. ORCID: https://orcid.org/0000-0001-5442-7702 <https://orcid.org/0000-0001-5442-7702> (2021) Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201. International Journal of Food Microbiology, 338. ISSN 0168-1605 https://www.scopus.com/redirect/linking.uri?targetURL=https%3a%2f%2fdoi.org%2f10.1016%2fj.ijfoodmicro.2020.108967&locationID=1&categoryID=4&eid=2-s2.0-85097072193&issn=01681605&linkType=ViewAtPublisher&year=2021&origin=recordpage&dig=0a9661152986edd2e285b35aff9c8e4c info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2020.108967
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QL Zoología
spellingShingle QL Zoología
Sevilla, Eloísa
Vico, Juan Pablo
Delgado-Blas, José F.
González-Zorn, Bruno
Marín, Clara M.
Uruén, Cristina
Martín-Burriel, Inmaculada
Bolea, Rosa
Mainar-Jaime, Raúl C.
Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201
topic_facet QL Zoología
description Salmonellosis is a common subclinical infection in pigs and therefore apparently healthy animals may represent a reservoir of antibiotic-resistant Salmonella for humans. This study estimates and characterizes resistance to two classes of antimicrobials considered of the highest priority within the critically important antimicrobials for humans, i.e. colistin (CR) and 3rd generation cephalosporins (3GC), on a collection of Salmonella isolates from pigs from two periods: between 2008 and 09, when colistin was massively used; and in 2018, after three years under a National Plan against Antibiotic Resistance. Prevalence of CR was low (6 out of 625; 0.96%; 95%CI: 0.44–2.1) in 2008–09 and associated mostly to the mcr-1 gene, which was detected in four S. 4,5,12:i:- isolates. Polymorphisms in the pmrAB genes were detected in a S. 9,12:-:- isolate. No CR was detected in 2018 out of 59 isolates tested. Among 270 Salmonella isolates considered for the assessment of resistance to 3GC in the 2008–2009 sampling, only one Salmonella Bredeney (0.37%; 95%CI: 0.07–2.1) showed resistance to 3GC, which was associated with the blaCMY-2 gene (AmpC producer). In 2018, six isolates out of 59 (10.2%; 95%CI: 4.7–20.5) showed resistance to 3GC, but only two different strains were identified (S. 4,12:i:- and S. Rissen), both confirmed as extended-spectrum β-lactamases (ESBL) producers. The blaCTX-M-3 and blaTEM-1b genes in S. 4,12:i:- and the blaTEM-1b gene in S. Rissen seemed to be associated with this resistance. Overall, the prevalence of CR in Salmonella appeared to be very low in 2008–2009 despite the considerable use of colistin in pigs at that time, and seemed to remain so in 2018. Resistance to 3GC was even lower in 2008–2009 but somewhat higher in 2018. Resistance was mostly coded by genes associated with mobile genetic elements. Most serotypes involved in these antimicrobial resistances displayed a multidrug resistance pattern and were considered zoonotic.
format Artículo
author Sevilla, Eloísa
Vico, Juan Pablo
Delgado-Blas, José F.
González-Zorn, Bruno
Marín, Clara M.
Uruén, Cristina
Martín-Burriel, Inmaculada
Bolea, Rosa
Mainar-Jaime, Raúl C.
author_facet Sevilla, Eloísa
Vico, Juan Pablo
Delgado-Blas, José F.
González-Zorn, Bruno
Marín, Clara M.
Uruén, Cristina
Martín-Burriel, Inmaculada
Bolea, Rosa
Mainar-Jaime, Raúl C.
author_sort Sevilla, Eloísa
title Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201
title_short Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201
title_full Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201
title_fullStr Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201
title_full_unstemmed Resistance to colistin and production of extended-spectrum β-lactamases and/or AmpC enzymes in Salmonella isolates collected from healthy pigs in Northwest Spain in two periods: 2008–2009 and 201
title_sort resistance to colistin and production of extended-spectrum β-lactamases and/or ampc enzymes in salmonella isolates collected from healthy pigs in northwest spain in two periods: 2008–2009 and 201
publisher Elsevier B.V.
publishDate 2021
url http://pa.bibdigital.ucc.edu.ar/3435/1/A_Sevilla_Vico_Delago-Blas.pdf
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