Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation

Giardia lamblia encodes several families of cysteine-rich proteins, including the Variant-specific Surface Proteins (VSPs) involved in the process of antigenic variation. Their characteristics, definition and relationships are still controversial. An exhaustive analysis of the Cys-rich families incl...

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Detalles Bibliográficos
Autores principales: Rodríguez-Walker, Macarena, Molina, Cecilia R., Luján, Lucas A., Saura, Alicia, Jerlström-Hultqvist, Jon, Svärd, Staffan G., Fernández, Elmer Andrés, Luján, Hugo Daniel
Formato: Artículo
Lenguaje:Español
Publicado: Academic Press Inc. 2022
Materias:
QD Química
R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3335/1/Rodriguez-Walker_Molina_Luj%C3%A1n.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3335
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
R Medicina (General)
spellingShingle QD Química
R Medicina (General)
Rodríguez-Walker, Macarena
Molina, Cecilia R.
Luján, Lucas A.
Saura, Alicia
Jerlström-Hultqvist, Jon
Svärd, Staffan G.
Fernández, Elmer Andrés
Luján, Hugo Daniel
Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation
topic_facet QD Química
R Medicina (General)
description Giardia lamblia encodes several families of cysteine-rich proteins, including the Variant-specific Surface Proteins (VSPs) involved in the process of antigenic variation. Their characteristics, definition and relationships are still controversial. An exhaustive analysis of the Cys-rich families including organization, features, evolution and levels of expression was performed, by combining pattern searches and predictions with massive sequencing techniques. Thus, a new classification for Cys-rich proteins, genes and pseudogenes that better describes their involvement in Giardia's biology is presented. Moreover, three novel characteristics exclusive to the VSP genes, comprising an Initiator element/Kozak-like sequence, an extended polyadenylation signal and a unique pattern of mutually exclusive transcript accumulation are presented, as well as the finding that High Cysteine Membrane Proteins, upregulated under stress, may protect the parasite during VSP switching. These results allow better interpretation of previous reports providing the basis for further studies of the biology of this early-branching eukaryote.
format Artículo
Artículo
author Rodríguez-Walker, Macarena
Molina, Cecilia R.
Luján, Lucas A.
Saura, Alicia
Jerlström-Hultqvist, Jon
Svärd, Staffan G.
Fernández, Elmer Andrés
Luján, Hugo Daniel
author_facet Rodríguez-Walker, Macarena
Molina, Cecilia R.
Luján, Lucas A.
Saura, Alicia
Jerlström-Hultqvist, Jon
Svärd, Staffan G.
Fernández, Elmer Andrés
Luján, Hugo Daniel
author_sort Rodríguez-Walker, Macarena
title Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation
title_short Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation
title_full Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation
title_fullStr Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation
title_full_unstemmed Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation
title_sort comprehensive characterization of cysteine-rich protein-coding genes of giardia lamblia and their role during antigenic variation
publisher Academic Press Inc.
publishDate 2022
url http://pa.bibdigital.ucc.edu.ar/3335/1/Rodriguez-Walker_Molina_Luj%C3%A1n.pdf
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