Decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis

Subclinical atherosclerosis (SCA) occurs in asymptomatic individuals. Blood peripheral monocytes are involved in the development of atherosclerosis. Circulating monocytes acquire pro-inflammatory profiles, and they are involved in the early stages of atherosclerosis development. Low-density lipoprot...

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Autores principales: Albertini, Ricardo A., Nicolas, Juan Carlos, Actis Dato, Virginia, Ferrer, Darío G., Tinti, María E., Capra, Raúl H., Chiabrando, Gustavo A.
Formato: Artículo
Lenguaje:Español
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3304/1/A_Albertini_Nicolas_Actis%20Dato.pdf
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id I38-R144-3304
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic Q Ciencia (General)
R Medicina (General)
spellingShingle Q Ciencia (General)
R Medicina (General)
Albertini, Ricardo A.
Nicolas, Juan Carlos
Actis Dato, Virginia
Ferrer, Darío G.
Tinti, María E.
Capra, Raúl H.
Chiabrando, Gustavo A.
Decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis
topic_facet Q Ciencia (General)
R Medicina (General)
description Subclinical atherosclerosis (SCA) occurs in asymptomatic individuals. Blood peripheral monocytes are involved in the development of atherosclerosis. Circulating monocytes acquire pro-inflammatory profiles, and they are involved in the early stages of atherosclerosis development. Low-density lipoprotein Receptor-related Protein 1 (LRP1) is expressed in monocytes, mainly in classical and intermediate subsets. Although LRP1 is highly expressed in macrophages and vascular smooth muscle cells (VSMCs) in atherosclerotic plaque formation, its expression in circulating monocytes has not been studied in SCA. The aim of this study was to characterize the LRP1 expression level in circulating monocytes of individuals with SCA and compared with individuals with low (LR) and intermediate (IR) risk of cardiovascular diseases, both without evidence of atherosclerotic lesions in carotid and coronary arteries. LRP1 and additional markers (CD11b, CD11c, and CD36) at cell surface of monocytes were analyzed by flow cytometry assays, whereas LRP1 and pro-inflammatory factors gene expressions were measured in isolated monocytes by quantitative RT-PCRs. Both LRP1 protein and LRP1 mRNA were significantly reduced in monocytes in SCA and IR respect to LR. Conversely, CD36, CD11b, and CD11c monocytic markers showed no significant changes between the different study groups. Finally, increased gene expressions of TNF-α and IL-1β were detected in monocytes of SCA, which were associated with decreased LRP1 expression at the cell surface in total monocytes. In summary, we propose that the decreased LRP1 expression at cell surface in total monocytes with pro-inflammatory profile is associated with the development of atherosclerosis in asymptomatic individuals.
format Artículo
Artículo
author Albertini, Ricardo A.
Nicolas, Juan Carlos
Actis Dato, Virginia
Ferrer, Darío G.
Tinti, María E.
Capra, Raúl H.
Chiabrando, Gustavo A.
author_facet Albertini, Ricardo A.
Nicolas, Juan Carlos
Actis Dato, Virginia
Ferrer, Darío G.
Tinti, María E.
Capra, Raúl H.
Chiabrando, Gustavo A.
author_sort Albertini, Ricardo A.
title Decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis
title_short Decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis
title_full Decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis
title_fullStr Decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis
title_full_unstemmed Decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis
title_sort decreased low-density lipoprotein receptor-related protein 1 expression in pro-inflammatory monocytes is associated with subclinical atherosclerosis
publisher Frontiers Media S.A.
publishDate 2022
url http://pa.bibdigital.ucc.edu.ar/3304/1/A_Albertini_Nicolas_Actis%20Dato.pdf
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