Intracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cells

Abstract: Cystic fibrosis (CF) is caused by mutations in the CFTR gene, which encodes a cAMP-regulated chloride channel. Several cellular functions are altered in CF cells. However, it is not clear how the CFTR failure induces those alterations. We have found previously several genes differentially...

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Autores principales: Clauzure, Mariángeles, Valdivieso, Ángel Gabriel, Massip Copiz, María Macarena, Mori, Consuelo, Dugour, Andrea V., Figueroa, Juan M., Santa Coloma, Tomás Antonio
Formato: Artículo
Lenguaje:Inglés
Publicado: Wiley 2019
Materias:
CELULAS EPITELIALES
MARCADORES GENETICOS
FIBROSIS QUISTICA
INFLAMACION
ANIONES
CLORURO INTRACELULAR
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8778
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8778
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic CELULAS EPITELIALES
MARCADORES GENETICOS
FIBROSIS QUISTICA
INFLAMACION
ANIONES
CLORURO INTRACELULAR
spellingShingle CELULAS EPITELIALES
MARCADORES GENETICOS
FIBROSIS QUISTICA
INFLAMACION
ANIONES
CLORURO INTRACELULAR
Clauzure, Mariángeles
Valdivieso, Ángel Gabriel
Massip Copiz, María Macarena
Mori, Consuelo
Dugour, Andrea V.
Figueroa, Juan M.
Santa Coloma, Tomás Antonio
Intracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cells
topic_facet CELULAS EPITELIALES
MARCADORES GENETICOS
FIBROSIS QUISTICA
INFLAMACION
ANIONES
CLORURO INTRACELULAR
description Abstract: Cystic fibrosis (CF) is caused by mutations in the CFTR gene, which encodes a cAMP-regulated chloride channel. Several cellular functions are altered in CF cells. However, it is not clear how the CFTR failure induces those alterations. We have found previously several genes differentially expressed in CF cells, including c-Src, MUC1, MTND4, and CISD1 (CFTR-dependent genes). Recently, we also reported the existence of several chloride-dependent genes, among them GLRX5 and RPS27. Here, varying the intracellular chloride concentration [Cl- ]i of IB3-1 CF bronchial epithelial cells, we show that IL-1β mRNA expression and secretion are also under Cl- modulation. The response to Cl- is biphasic, with maximal effects at 75 mM Cl- . The regulation of the IL-1β mRNA expression involves an IL-1β autocrine effect, since in the presence of the IL-1β receptor antagonist IL1RN or anti-IL-1β blocking antibody, the mRNA response to Cl- disappeared. Similar effects were obtained with the JNK inhibitor SP600125, the c-Src inhibitor PP2 and the IKK inhibitor III (BMS-345541). On the other hand, the IL-1β secretion is still modulated by Cl- in the presence of IL-1RN, IL-1β blocking antibody, or cycloheximide, suggesting that Cl- is affecting the IL-1β maturation/secretion, which in turn starts an autocrine positive feedback loop. In conclusion, the Cl- anion acts as a second messenger for CFTR, modulating the IL-1β maturation/secretion. The results also imply that, depending on its intracellular concentration, Cl- could be a pro-inflammatory mediator. J. Cell. Biochem. 118: 2131-2140, 2017. © 2016 Wiley Periodicals, Inc.
format Artículo
author Clauzure, Mariángeles
Valdivieso, Ángel Gabriel
Massip Copiz, María Macarena
Mori, Consuelo
Dugour, Andrea V.
Figueroa, Juan M.
Santa Coloma, Tomás Antonio
author_facet Clauzure, Mariángeles
Valdivieso, Ángel Gabriel
Massip Copiz, María Macarena
Mori, Consuelo
Dugour, Andrea V.
Figueroa, Juan M.
Santa Coloma, Tomás Antonio
author_sort Clauzure, Mariángeles
title Intracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cells
title_short Intracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cells
title_full Intracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cells
title_fullStr Intracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cells
title_full_unstemmed Intracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cells
title_sort intracellular chloride concentration changes modulate il-1β expression and secretion in human bronchial epithelial cultured cells
publisher Wiley
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8778
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