Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells

Abstract: Depletion of Ca2+ stores activates store-operated channels (SOCs), which mediate Ca2+ entry pathways that regulate cellular processes such as contraction, proliferation and gene expression. In vascular smooth muscle cells (VSMCs), stimulation of SOCs composed of canonical transient recepto...

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Detalles Bibliográficos
Autores principales: Shi, Jian, Miralles, Francesc, Birnbaumer, Lutz, Large, William A., Albert, Anthony P.
Otros Autores: 0000-0002-3596-9634
Formato: Artículo
Lenguaje:Inglés
Publicado: Wiley 2019
Materias:
MALFORMACIONES VASCULARES
MUSCULOS
PROTEINAS
CALCIO
MARCADORES BIOLOGICOS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8761
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8761
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic MALFORMACIONES VASCULARES
MUSCULOS
PROTEINAS
CALCIO
MARCADORES BIOLOGICOS
spellingShingle MALFORMACIONES VASCULARES
MUSCULOS
PROTEINAS
CALCIO
MARCADORES BIOLOGICOS
Shi, Jian
Miralles, Francesc
Birnbaumer, Lutz
Large, William A.
Albert, Anthony P.
Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells
topic_facet MALFORMACIONES VASCULARES
MUSCULOS
PROTEINAS
CALCIO
MARCADORES BIOLOGICOS
description Abstract: Depletion of Ca2+ stores activates store-operated channels (SOCs), which mediate Ca2+ entry pathways that regulate cellular processes such as contraction, proliferation and gene expression. In vascular smooth muscle cells (VSMCs), stimulation of SOCs composed of canonical transient receptor potential channel 1 (TRPC1) proteins requires G protein α q subunit (Gαq)/phospholipase C (PLC)β1/protein kinase C (PKC) activity. We studied the role of stromal interaction molecule 1 (STIM1) in coupling store depletion to this activation pathway using patch clamp recording, GFP-PLCδ1-PH imaging and co-localization techniques. Store-operated TRPC1 channel and PLCβ1 activities were inhibited by STIM1 short hairpin RNA (shRNA) and absent in TRPC1-/- cells, and store-operated PKC phosphorylation of TRPC1 was inhibited by STIM1 shRNA. Store depletion induced interactions between STIM1 and TRPC1, Gαq and PLCβ1, which required STIM1 and TRPC1. Similar effects were produced with noradrenaline. These findings identify a new activation mechanism of TRPC1-based SOCs in VSMCs, and a novel role for STIM1, where store-operated STIM1-TRPC1 interactions stimulate Gαq/PLCβ1/PKC activity to induce channel gating.
author2 0000-0002-3596-9634
author_facet 0000-0002-3596-9634
Shi, Jian
Miralles, Francesc
Birnbaumer, Lutz
Large, William A.
Albert, Anthony P.
format Artículo
author Shi, Jian
Miralles, Francesc
Birnbaumer, Lutz
Large, William A.
Albert, Anthony P.
author_sort Shi, Jian
title Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells
title_short Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells
title_full Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells
title_fullStr Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells
title_full_unstemmed Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells
title_sort store-operated interactions between plasmalemmal stim1 and trpc1 proteins stimulate plcβ1 to induce trpc1 channel activation in vascular smooth muscle cells
publisher Wiley
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8761
work_keys_str_mv AT shijian storeoperatedinteractionsbetweenplasmalemmalstim1andtrpc1proteinsstimulateplcb1toinducetrpc1channelactivationinvascularsmoothmusclecells
AT mirallesfrancesc storeoperatedinteractionsbetweenplasmalemmalstim1andtrpc1proteinsstimulateplcb1toinducetrpc1channelactivationinvascularsmoothmusclecells
AT birnbaumerlutz storeoperatedinteractionsbetweenplasmalemmalstim1andtrpc1proteinsstimulateplcb1toinducetrpc1channelactivationinvascularsmoothmusclecells
AT largewilliama storeoperatedinteractionsbetweenplasmalemmalstim1andtrpc1proteinsstimulateplcb1toinducetrpc1channelactivationinvascularsmoothmusclecells
AT albertanthonyp storeoperatedinteractionsbetweenplasmalemmalstim1andtrpc1proteinsstimulateplcb1toinducetrpc1channelactivationinvascularsmoothmusclecells
bdutipo_str Repositorios
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