Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus

Abstract: The effect of cadmium (Cd) in the brain has been attributed to an increase in reactive oxygen species in cells, particularly when high amounts of the metal are given. In this study we examined the effect of a low dose of Cd (7.5 μg/day) on 24-h changes in expression of redox pathway enzyme...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Jiménez Ortega, Vanesa, Cardinali, Daniel Pedro, Fernández Mateos, María P., Ríos Lugo, María J., Scacchi, Pablo, Esquifino, Ana I.
Formato: Artículo
Lenguaje:Inglés
Inglés
Publicado: Springer 2019
Materias:
RITMO CIRCADIANO
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
SUPEROXIDO DISMUTASA
CATALASA
GLUTATION PEROXIDASA
GLUTATION REDUCTASA
CADMIO
HIPOTALAMO MEDIO
EXPRESION GENICA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/1643
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-1643
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
Inglés
topic RITMO CIRCADIANO
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
SUPEROXIDO DISMUTASA
CATALASA
GLUTATION PEROXIDASA
GLUTATION REDUCTASA
CADMIO
HIPOTALAMO MEDIO
EXPRESION GENICA
spellingShingle RITMO CIRCADIANO
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
SUPEROXIDO DISMUTASA
CATALASA
GLUTATION PEROXIDASA
GLUTATION REDUCTASA
CADMIO
HIPOTALAMO MEDIO
EXPRESION GENICA
Jiménez Ortega, Vanesa
Cardinali, Daniel Pedro
Fernández Mateos, María P.
Ríos Lugo, María J.
Scacchi, Pablo
Esquifino, Ana I.
Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus
topic_facet RITMO CIRCADIANO
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
SUPEROXIDO DISMUTASA
CATALASA
GLUTATION PEROXIDASA
GLUTATION REDUCTASA
CADMIO
HIPOTALAMO MEDIO
EXPRESION GENICA
description Abstract: The effect of cadmium (Cd) in the brain has been attributed to an increase in reactive oxygen species in cells, particularly when high amounts of the metal are given. In this study we examined the effect of a low dose of Cd (7.5 μg/day) on 24-h changes in expression of redox pathway enzyme and circadian genes in rat medial basal hypothalamus (MBH). Rats receiving CdCl2 (5 ppm in drinking water) or tap water for one month were killed at 6 different time intervals throughout a 24 h cycle. MBH mRNA levels were measured by real-time PCR analysis. In CdCl2 treated rats a disruption of 24-h pattern of hypothalamic gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase (HO)-1 and -2, Mn- superoxide dismutase (SOD), catalase, glutathione peroxidase and glutathione reductase was detectable. Mean levels of MBH mRNA for HO-2, Mn-SOD and catalase augmented after Cd intake, whereas those of NOS-2 decreased. After CdCl2 intake rats the 24-h pattern of clock gene expression in MBH seen in controls was significantly suppressed (Bmal1) or changed in phase (Per1, Per2, Cry2) while in the case of Clock significant 24-h variations were induced. The results are compatible with the view that a low amount of Cd given in tap water brought about significant changes in circadian expression of redox enzyme and clock genes in rat MBH.
format Artículo
author Jiménez Ortega, Vanesa
Cardinali, Daniel Pedro
Fernández Mateos, María P.
Ríos Lugo, María J.
Scacchi, Pablo
Esquifino, Ana I.
author_facet Jiménez Ortega, Vanesa
Cardinali, Daniel Pedro
Fernández Mateos, María P.
Ríos Lugo, María J.
Scacchi, Pablo
Esquifino, Ana I.
author_sort Jiménez Ortega, Vanesa
title Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus
title_short Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus
title_full Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus
title_fullStr Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus
title_full_unstemmed Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus
title_sort effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus
publisher Springer
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/1643
work_keys_str_mv AT jimenezortegavanesa effectofcadmiumon24hourpatterninexpressionofredoxenzimeandclockgenesinratmedialbasalhypothalamus
AT cardinalidanielpedro effectofcadmiumon24hourpatterninexpressionofredoxenzimeandclockgenesinratmedialbasalhypothalamus
AT fernandezmateosmariap effectofcadmiumon24hourpatterninexpressionofredoxenzimeandclockgenesinratmedialbasalhypothalamus
AT rioslugomariaj effectofcadmiumon24hourpatterninexpressionofredoxenzimeandclockgenesinratmedialbasalhypothalamus
AT scacchipablo effectofcadmiumon24hourpatterninexpressionofredoxenzimeandclockgenesinratmedialbasalhypothalamus
AT esquifinoanai effectofcadmiumon24hourpatterninexpressionofredoxenzimeandclockgenesinratmedialbasalhypothalamus
bdutipo_str Repositorios
_version_ 1764820525767458819

Ejemplares similares