Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin

Abstract: In a previous study we reported that a low daily p.o. dose of cadmium (Cd) disrupted the circadian expression of clock and redox enzyme genes in rat medial basal hypothalamus (MBH).To assess whether melatonin could counteract Cd activity, maleWistar rats (45 days of age) received CdCl2 (5...

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Autores principales: Jiménez Ortega, Vanesa, Cano Barquilla, Pilar, Scacchi Bernasconi, Pablo A., Cardinali, Daniel Pedro, Esquifino, Ana I.
Formato: Artículo
Lenguaje:Inglés
Inglés
Publicado: Frontiers In 2019
Materias:
MELATONINA
CADMIO
RITMO CIRCADIANO
HIPOTALAMO MEDIO
GENES RELOJ
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/1641
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-1641
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
Inglés
topic MELATONINA
CADMIO
RITMO CIRCADIANO
HIPOTALAMO MEDIO
GENES RELOJ
spellingShingle MELATONINA
CADMIO
RITMO CIRCADIANO
HIPOTALAMO MEDIO
GENES RELOJ
Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Scacchi Bernasconi, Pablo A.
Cardinali, Daniel Pedro
Esquifino, Ana I.
Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin
topic_facet MELATONINA
CADMIO
RITMO CIRCADIANO
HIPOTALAMO MEDIO
GENES RELOJ
description Abstract: In a previous study we reported that a low daily p.o. dose of cadmium (Cd) disrupted the circadian expression of clock and redox enzyme genes in rat medial basal hypothalamus (MBH).To assess whether melatonin could counteract Cd activity, maleWistar rats (45 days of age) received CdCl2 (5 ppm) and melatonin (3μg/mL) or vehicle (0.015%ethanol) in drinking water. Groups of animals receiving melatonin or vehicle alone were also included. After 1 month, MBH mRNA levels were measured by real-time PCR analysis at six time intervals in a 24-h cycle. In control MBH Bmal1 expression peaked at early scotophase, Per1 expression at late afternoon, and Per2 and Cry2 expression at mid-scotophase, whereas neither Clock nor Cry1 expression showed significant 24-h variations.This pattern was significantly disrupted (Clock, Bmal1) or changed in phase (Per1, Per2, Cry2) by CdCl2 while melatonin counteracted the changes brought about by Cd on Per1 expression only. In animals receiving melatonin alone the 24-h pattern of MBH Per2 and Cry2 expression was disrupted. CdCl2 disrupted the 24-h rhythmicity of Cu/Zn- and Mn-superoxide dismutase (SOD), nitric oxide synthase (NOS)-1, NOS-2, heme oxygenase (HO)-1, and HO-2 gene expression, most of the effects being counteracted by melatonin. In particular, the co-administration of melatonin and CdCl2 increased Cu/Zn-SOD gene expression and decreased that of glutathione peroxidase (GPx), glutathione reductase (GSR), and HO-2. In animals receiving melatonin alone, significant increases in mean Cu/Zn and Mn-SOD gene expression, and decreases in that of GPx, GSR, NOS-1, NOS-2, HO-1, and HO-2, were found. The results indicate that the interfering effect of melatonin on the activity of a low dose of CdCl2 on MBH clock and redox enzyme genes is mainly exerted at the level of redox enzyme gene expression.
format Artículo
author Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Scacchi Bernasconi, Pablo A.
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_facet Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Scacchi Bernasconi, Pablo A.
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_sort Jiménez Ortega, Vanesa
title Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin
title_short Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin
title_full Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin
title_fullStr Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin
title_full_unstemmed Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin
title_sort cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus : prevention by melatonin
publisher Frontiers In
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/1641
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