Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528

Abstract: Enzymes from cold-adapted microorganisms are of high interest to industries due to their high activity at low and mild temperatures, which makes them suitable for their use in several processes that either require a supply of exogenous energy or involve the use of heat labile products....

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Detalles Bibliográficos
Autores principales: Lario, Luciana Daniela, Pillaca-Pullo, Omar Santiago, Durães Sette, Lara, Converti, Attilio, Casati, Paula, Spampinato, Claudia, Pessoa, Adalberto
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier B. V. 2020
Materias:
PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/10930
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-10930
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
spellingShingle PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
Lario, Luciana Daniela
Pillaca-Pullo, Omar Santiago
Durães Sette, Lara
Converti, Attilio
Casati, Paula
Spampinato, Claudia
Pessoa, Adalberto
Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
topic_facet PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
description Abstract: Enzymes from cold-adapted microorganisms are of high interest to industries due to their high activity at low and mild temperatures, which makes them suitable for their use in several processes that either require a supply of exogenous energy or involve the use of heat labile products. In this work, the protease production by the strain Rhodotorula mucilaginosa CBMAI 1528, previously isolated from the Antarctic continent, was optimized, and the purified enzyme analyzed. It was found that protease production was dependent on culture medium composition and growth temperature, being 20 ºC and a culture medium containing both glucose and casein peptone (20 and 10 g/L, respectively) the optimal growing conditions in batch as well as in bioreactor.
format Artículo
author Lario, Luciana Daniela
Pillaca-Pullo, Omar Santiago
Durães Sette, Lara
Converti, Attilio
Casati, Paula
Spampinato, Claudia
Pessoa, Adalberto
author_facet Lario, Luciana Daniela
Pillaca-Pullo, Omar Santiago
Durães Sette, Lara
Converti, Attilio
Casati, Paula
Spampinato, Claudia
Pessoa, Adalberto
author_sort Lario, Luciana Daniela
title Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_short Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_full Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_fullStr Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_full_unstemmed Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_sort optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast rhodotorula mucilaginosa cbmai 1528
publisher Elsevier B. V.
publishDate 2020
url https://repositorio.uca.edu.ar/handle/123456789/10930
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