The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI

Resumen: Members of the Equus genus exhibit a fascinating capacity for hybridization, giving rise to healthy offspring. Mules, resulting from the mating of a mare with a jack, represent the most prevalent equid hybrid, serving diverse roles in our society. While in vitro embryo production, particu...

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Autores principales: Arroyo Salvo, Camila, Cogollo Villarreal, María Y., Clérico, Gabriel, Flores Bragulat, Ana P., Niño Vargas, Andrea, Castañeira, Catalina, Briski, Olinda, Alonso, Carolina, Pérez Martínez, Silvina, Gambini, Andrés
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2024
Materias:
PRODUCCION DE HIBRIDOS
EQUINOS
FERTILIZACION IN VITRO
PRESERVACION GENÉTICA
INYECCIÓN DE ESPERMA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/17761
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-17761
record_format dspace
spelling I33-R139-123456789-177612024-03-27T15:14:03Z The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI Arroyo Salvo, Camila Cogollo Villarreal, María Y. Clérico, Gabriel Flores Bragulat, Ana P. Niño Vargas, Andrea Castañeira, Catalina Briski, Olinda Alonso, Carolina Pérez Martínez, Silvina Gambini, Andrés PRODUCCION DE HIBRIDOS EQUINOS FERTILIZACION IN VITRO PRESERVACION GENÉTICA INYECCIÓN DE ESPERMA Resumen: Members of the Equus genus exhibit a fascinating capacity for hybridization, giving rise to healthy offspring. Mules, resulting from the mating of a mare with a jack, represent the most prevalent equid hybrid, serving diverse roles in our society. While in vitro embryo production, particularly through Intracytoplasmic Sperm Injection (ICSI), has rapidly gained significance in domestic horses, the in vitro production in other equids remains largely unexplored. Utilizing donkey sperm for fertilizing horse oocytes not only addresses this gap but also provides an opportunity to investigate donkey sperm’s fertilization capability in vitro to further improve donkey ICSI. In this work, we initially studied the localization of donkey sperm Phospholipase C zeta (PLCζ) and assessed the sperm’s capacity to induce pronuclear formation and maternal SMARCA4 recruitment upon injection into pig oocytes through ICSI. Subsequently, we investigated the injection of donkey sperm into horse oocytes, evaluating in vitro production up to the blastocyst stage using sperm from different jacks, including frozen and refrigerated samples. Distinct patterns of PLCζ localization were observed for donkey sperm cells compared to their horse counterparts. Additionally, donkey sperm exhibits a reduced ability to induce porcine oocyte activation. However, when injected into horse oocytes, donkey sperm demonstrated sufficient capability to induce oocyte activation as no discernible differences in cleavage or blastocyst rates are observed between in vitro produced mules and horse ICSI embryos. Our study not only delineates PLCζ localization in donkey sperm but also suggests potential differences in the ability to induce oocyte activation in pigs compared to horses while observing no distinctions in pronuclear recruitment of SMARCA4. Interestingly, donkey sperm remains sufficiently capable of inducing horse oocyte activation for in vitro mule blastocyst production. 2024-02-20T14:53:41Z 2024-02-20T14:53:41Z 2024 Artículo Arroyo Salvo, C. et al. The ability of donkey sperm to induce oocyte activation and mule embryo development after [en línea]. Theriogenology. 2024, 2. doi: 10.1016/j.theriogenology.2024.02.002. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17761 1879- 3231 https://repositorio.uca.edu.ar/handle/123456789/17761 10.1016/j.theriogenology.2024.02.002 eng Producción de embriones intra e interespecíficos en el modelo ICSI en équidos Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Elsevier Theriogenology. 2024, 2
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic PRODUCCION DE HIBRIDOS
EQUINOS
FERTILIZACION IN VITRO
PRESERVACION GENÉTICA
INYECCIÓN DE ESPERMA
spellingShingle PRODUCCION DE HIBRIDOS
EQUINOS
FERTILIZACION IN VITRO
PRESERVACION GENÉTICA
INYECCIÓN DE ESPERMA
Arroyo Salvo, Camila
Cogollo Villarreal, María Y.
Clérico, Gabriel
Flores Bragulat, Ana P.
Niño Vargas, Andrea
Castañeira, Catalina
Briski, Olinda
Alonso, Carolina
Pérez Martínez, Silvina
Gambini, Andrés
The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI
topic_facet PRODUCCION DE HIBRIDOS
EQUINOS
FERTILIZACION IN VITRO
PRESERVACION GENÉTICA
INYECCIÓN DE ESPERMA
description Resumen: Members of the Equus genus exhibit a fascinating capacity for hybridization, giving rise to healthy offspring. Mules, resulting from the mating of a mare with a jack, represent the most prevalent equid hybrid, serving diverse roles in our society. While in vitro embryo production, particularly through Intracytoplasmic Sperm Injection (ICSI), has rapidly gained significance in domestic horses, the in vitro production in other equids remains largely unexplored. Utilizing donkey sperm for fertilizing horse oocytes not only addresses this gap but also provides an opportunity to investigate donkey sperm’s fertilization capability in vitro to further improve donkey ICSI. In this work, we initially studied the localization of donkey sperm Phospholipase C zeta (PLCζ) and assessed the sperm’s capacity to induce pronuclear formation and maternal SMARCA4 recruitment upon injection into pig oocytes through ICSI. Subsequently, we investigated the injection of donkey sperm into horse oocytes, evaluating in vitro production up to the blastocyst stage using sperm from different jacks, including frozen and refrigerated samples. Distinct patterns of PLCζ localization were observed for donkey sperm cells compared to their horse counterparts. Additionally, donkey sperm exhibits a reduced ability to induce porcine oocyte activation. However, when injected into horse oocytes, donkey sperm demonstrated sufficient capability to induce oocyte activation as no discernible differences in cleavage or blastocyst rates are observed between in vitro produced mules and horse ICSI embryos. Our study not only delineates PLCζ localization in donkey sperm but also suggests potential differences in the ability to induce oocyte activation in pigs compared to horses while observing no distinctions in pronuclear recruitment of SMARCA4. Interestingly, donkey sperm remains sufficiently capable of inducing horse oocyte activation for in vitro mule blastocyst production.
format Artículo
author Arroyo Salvo, Camila
Cogollo Villarreal, María Y.
Clérico, Gabriel
Flores Bragulat, Ana P.
Niño Vargas, Andrea
Castañeira, Catalina
Briski, Olinda
Alonso, Carolina
Pérez Martínez, Silvina
Gambini, Andrés
author_facet Arroyo Salvo, Camila
Cogollo Villarreal, María Y.
Clérico, Gabriel
Flores Bragulat, Ana P.
Niño Vargas, Andrea
Castañeira, Catalina
Briski, Olinda
Alonso, Carolina
Pérez Martínez, Silvina
Gambini, Andrés
author_sort Arroyo Salvo, Camila
title The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI
title_short The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI
title_full The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI
title_fullStr The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI
title_full_unstemmed The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI
title_sort ability of donkey sperm to induce oocyte activation and mule embryo development after icsi
publisher Elsevier
publishDate 2024
url https://repositorio.uca.edu.ar/handle/123456789/17761
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