Single-molecule localization super-resolution microscopy of synaptic proteins

Abstract: Recent years have witnessed huge progress in the field of light microscopy with the development and implementation of new approaches leading to dramatic improvements in the spatial and temporal resolution of this form of imaging, most particularly in its biological applications. The limi...

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Detalles Bibliográficos
Autor principal: Barrantes, Francisco José
Formato: Parte de libro
Lenguaje:Español
Publicado: Humana Press 2022
Materias:
NANOSCOPIA
TINCION
CELULAS NEURONALES
MICROSCOPIA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/15340
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-15340
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Español
topic NANOSCOPIA
TINCION
CELULAS NEURONALES
MICROSCOPIA
spellingShingle NANOSCOPIA
TINCION
CELULAS NEURONALES
MICROSCOPIA
Barrantes, Francisco José
Single-molecule localization super-resolution microscopy of synaptic proteins
topic_facet NANOSCOPIA
TINCION
CELULAS NEURONALES
MICROSCOPIA
description Abstract: Recent years have witnessed huge progress in the field of light microscopy with the development and implementation of new approaches leading to dramatic improvements in the spatial and temporal resolution of this form of imaging, most particularly in its biological applications. The limitations in spatial resolution imposed by the diffraction of light have been circumvented by resorting to different strategies, which are briefly outlined in the Introduction. These protocols are intended to provide practical guidelines for the imaging of synaptic proteins using one such strategy, namely, single-molecule stochastic localization super-resolution microscopy. The protocols use neuronal cells from the hippocampus of rodent embryos as the experimental paradigm and outline the steps for obtaining dissociated neurons and establishing primary cultures for in vitro studies. The techniques can be adapted to the culture of neurons from other brain regions. Procedures for handling fixed and live specimens are described, as well as the use of extrinsic fluorescent probes and fluorescent proteins, mounting media, examples of hardware configurations, software for image analysis, and some hints for the implementation of minimalist approaches to single-molecule localization nanoscopy.
format Parte de libro
author Barrantes, Francisco José
author_facet Barrantes, Francisco José
author_sort Barrantes, Francisco José
title Single-molecule localization super-resolution microscopy of synaptic proteins
title_short Single-molecule localization super-resolution microscopy of synaptic proteins
title_full Single-molecule localization super-resolution microscopy of synaptic proteins
title_fullStr Single-molecule localization super-resolution microscopy of synaptic proteins
title_full_unstemmed Single-molecule localization super-resolution microscopy of synaptic proteins
title_sort single-molecule localization super-resolution microscopy of synaptic proteins
publisher Humana Press
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/15340
work_keys_str_mv AT barrantesfranciscojose singlemoleculelocalizationsuperresolutionmicroscopyofsynapticproteins
bdutipo_str Repositorios
_version_ 1764820523681841158

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