Polyphenol-rich fraction from Larrea divaricata and its main flavonoid Quercetin-3-Methyl Ether induce apoptosis in lymphoma cells through nitrosative stress

Abstract: Larrea divaricata is a plant with antiproliferative principles. We have previously identified the flavonoid quercetin-3-methyl ether (Q-3-ME) in an ethyl acetate fraction (EA). Both the extract and Q-3-ME were found to be effective against the EL-4 T lymphoma cell line. However, the mechan...

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Detalles Bibliográficos
Autores principales: Martino, Renzo, Barreiro Arcos, María Laura, Alonso, Rosario, Sülsen, Valeria, Cremaschi, Graciela A., Anesini, Claudia
Formato: Artículo
Lenguaje:Inglés
Publicado: Wiley 2022
Materias:
APOPTOSIS
LARREA DIVARICATA
LINFOMA DE CELULAS T
ETIL ACETATO
OXIDO NITRICO
POLIFENOLES
QUERCETINA
LINFOMAS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/15337
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-15337
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic APOPTOSIS
LARREA DIVARICATA
LINFOMA DE CELULAS T
ETIL ACETATO
OXIDO NITRICO
POLIFENOLES
QUERCETINA
LINFOMAS
spellingShingle APOPTOSIS
LARREA DIVARICATA
LINFOMA DE CELULAS T
ETIL ACETATO
OXIDO NITRICO
POLIFENOLES
QUERCETINA
LINFOMAS
Martino, Renzo
Barreiro Arcos, María Laura
Alonso, Rosario
Sülsen, Valeria
Cremaschi, Graciela A.
Anesini, Claudia
Polyphenol-rich fraction from Larrea divaricata and its main flavonoid Quercetin-3-Methyl Ether induce apoptosis in lymphoma cells through nitrosative stress
topic_facet APOPTOSIS
LARREA DIVARICATA
LINFOMA DE CELULAS T
ETIL ACETATO
OXIDO NITRICO
POLIFENOLES
QUERCETINA
LINFOMAS
description Abstract: Larrea divaricata is a plant with antiproliferative principles. We have previously identified the flavonoid quercetin-3-methyl ether (Q-3-ME) in an ethyl acetate fraction (EA). Both the extract and Q-3-ME were found to be effective against the EL-4 T lymphoma cell line. However, the mechanism underlying the inhibition of tumor cell proliferation remains to be elucidated. In this work, we analyzed the role of nitric oxide (NO) in the induction of apoptosis mediated by Q-3-ME and EA. Both treatments were able to induce apoptosis in a concentration-dependent and time-dependent manner. The western blot analysis revealed a sequential activation of caspases-9 and 3, followed by poly-(ADP-ribose)-polymerase cleavage. EA and Q-3-ME lowered the mitochondrial membrane potential, showing the activation of the intrinsic pathway of apoptosis. Q-3-ME and EA increased NO production and inducible NO synthase expression in tumor cells. The involvement of NO in cell death was confirmed by the nitric oxide synthases inhibitor L-NAME. In addition, EA and Q-3-ME induced a cell cycle arrest in G0/G1 phase. These drugs did not affect normal cell viability. This data suggested that EA and Q-3-ME induce an increase in NO production that would lead to the cell cycle arrest and the activation of the intrinsic pathway of apoptosis. Copyright © 2016 John Wiley & Sons, Ltd.
format Artículo
author Martino, Renzo
Barreiro Arcos, María Laura
Alonso, Rosario
Sülsen, Valeria
Cremaschi, Graciela A.
Anesini, Claudia
author_facet Martino, Renzo
Barreiro Arcos, María Laura
Alonso, Rosario
Sülsen, Valeria
Cremaschi, Graciela A.
Anesini, Claudia
author_sort Martino, Renzo
title Polyphenol-rich fraction from Larrea divaricata and its main flavonoid Quercetin-3-Methyl Ether induce apoptosis in lymphoma cells through nitrosative stress
title_short Polyphenol-rich fraction from Larrea divaricata and its main flavonoid Quercetin-3-Methyl Ether induce apoptosis in lymphoma cells through nitrosative stress
title_full Polyphenol-rich fraction from Larrea divaricata and its main flavonoid Quercetin-3-Methyl Ether induce apoptosis in lymphoma cells through nitrosative stress
title_fullStr Polyphenol-rich fraction from Larrea divaricata and its main flavonoid Quercetin-3-Methyl Ether induce apoptosis in lymphoma cells through nitrosative stress
title_full_unstemmed Polyphenol-rich fraction from Larrea divaricata and its main flavonoid Quercetin-3-Methyl Ether induce apoptosis in lymphoma cells through nitrosative stress
title_sort polyphenol-rich fraction from larrea divaricata and its main flavonoid quercetin-3-methyl ether induce apoptosis in lymphoma cells through nitrosative stress
publisher Wiley
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/15337
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