Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase

Abstract: Thyroid hormones are important regulators of cell physiology, inducing cell proliferation, differentiation or apoptosis, depending on the cell type. Thyroid hormones induce proliferation in short-term T lymphocyte cultures. In this study, we assessed the effect of long-term thyroxine (T4)...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Barreiro Arcos, María Laura, Sterle, Helena Andrea, Vercelli, C., Valli, Eduardo, Cayrol, María Florencia, Klecha, Alicia Juana, Paulazo, Maria Alejandra, Díaz Flaqué, María Celeste, Franchi, A. M., Cremaschi, Graciela A.
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2022
Materias:
APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/15265
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-15265
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
spellingShingle APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
Barreiro Arcos, María Laura
Sterle, Helena Andrea
Vercelli, C.
Valli, Eduardo
Cayrol, María Florencia
Klecha, Alicia Juana
Paulazo, Maria Alejandra
Díaz Flaqué, María Celeste
Franchi, A. M.
Cremaschi, Graciela A.
Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
topic_facet APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
description Abstract: Thyroid hormones are important regulators of cell physiology, inducing cell proliferation, differentiation or apoptosis, depending on the cell type. Thyroid hormones induce proliferation in short-term T lymphocyte cultures. In this study, we assessed the effect of long-term thyroxine (T4) treatment on the balance of proliferation and apoptosis and the intermediate participants in T lymphoma cells. Treatment with T4 affected this balance from the fifth day of culture, inhibiting proliferation in a time-dependent manner. This effect was associated with apoptosis induction, as characterized through nuclear morphological changes, DNA fragmentation, and Annexin V-FITC/Propidium Iodide co-staining. In addition, increased iNOS gene and protein levels, and enzyme activity were observed. The generation of reactive oxygen species, depolarization of the mitochondrial membrane, and a reduction in glutathione levels were also observed. The imbalance between oxidants and antioxidants species is typically associated with the nitration of proteins, including PKCζ, an isoenzyme essential for lymphoma cell division and survival. Consistently, evidence of PKCζ nitration via proteasome degradation was also observed in this study. Taken together, these results suggest that the long-term culture of T lymphoma cells with T4 induces apoptosis through the increased production of oxidative species resulting from both augmented iNOS activity and the loss of mitochondrial function. These species induce the nitration of proteins involved in cell viability, promoting proteasome degradation. Furthermore, we discuss the impact of these results on the modulation of T lymphoma growth and the thyroid status in vivo.
format Artículo
author Barreiro Arcos, María Laura
Sterle, Helena Andrea
Vercelli, C.
Valli, Eduardo
Cayrol, María Florencia
Klecha, Alicia Juana
Paulazo, Maria Alejandra
Díaz Flaqué, María Celeste
Franchi, A. M.
Cremaschi, Graciela A.
author_facet Barreiro Arcos, María Laura
Sterle, Helena Andrea
Vercelli, C.
Valli, Eduardo
Cayrol, María Florencia
Klecha, Alicia Juana
Paulazo, Maria Alejandra
Díaz Flaqué, María Celeste
Franchi, A. M.
Cremaschi, Graciela A.
author_sort Barreiro Arcos, María Laura
title Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_short Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_full Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_fullStr Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_full_unstemmed Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_sort induction of apoptosis in t lymphoma cells by long-term treatment with thyroxine involves pkcf nitration by nitric oxide synthase
publisher Springer
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/15265
work_keys_str_mv AT barreiroarcosmarialaura inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT sterlehelenaandrea inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT vercellic inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT vallieduardo inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT cayrolmariaflorencia inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT klechaaliciajuana inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT paulazomariaalejandra inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT diazflaquemariaceleste inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT franchiam inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
AT cremaschigracielaa inductionofapoptosisintlymphomacellsbylongtermtreatmentwiththyroxineinvolvespkcfnitrationbynitricoxidesynthase
bdutipo_str Repositorios
_version_ 1764820525707689989

Ejemplares similares