Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor

Abstract: Diacylglycerol (DAG), a second messenger involved in different cell signaling cascades, activates protein kinase C (PKC) and D (PKD), among other kinases. The present work analyzes the effects resulting from the alteration of DAG levels on neuronal and muscle nicotinic acetylcholine recept...

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Detalles Bibliográficos
Autores principales: Kamerbeek, Constanza B., Mateos, Melina V., Vallés, Ana Sofía, Pediconi, María F., Barrantes, Francisco José, Borroni, María Virginia
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/15214
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-15214
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
spellingShingle DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
Kamerbeek, Constanza B.
Mateos, Melina V.
Vallés, Ana Sofía
Pediconi, María F.
Barrantes, Francisco José
Borroni, María Virginia
Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
topic_facet DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
description Abstract: Diacylglycerol (DAG), a second messenger involved in different cell signaling cascades, activates protein kinase C (PKC) and D (PKD), among other kinases. The present work analyzes the effects resulting from the alteration of DAG levels on neuronal and muscle nicotinic acetylcholine receptor (AChR) distribution. We employ CHO-K1/A5 cells, expressing adult muscle-type AChR in a stable manner, and hippocampal neurons, which endogenously express various subtypes of neuronal AChR. CHO-K1/A5 cells treated with dioctanoylglycerol (DOG) for different periods showed augmented AChR cell surface levels at short incubation times (30min-4h) whereas at longer times (18h) the AChR was shifted to intracellular compartments. Similarly, in cultured hippocampal neurons surface AChR levels increased as a result of DOG incubation for 4h. Inhibition of endogenous DAG catabolism produced changes in AChR distribution similar to those induced by DOG treatment. Specific enzyme inhibitors and Western blot assays revealed that DAGs exert their effect on AChR distribution through the modulation of the activity of classical PKC (cPKC), novel PKC (nPKC) and PKD activity.
format Artículo
author Kamerbeek, Constanza B.
Mateos, Melina V.
Vallés, Ana Sofía
Pediconi, María F.
Barrantes, Francisco José
Borroni, María Virginia
author_facet Kamerbeek, Constanza B.
Mateos, Melina V.
Vallés, Ana Sofía
Pediconi, María F.
Barrantes, Francisco José
Borroni, María Virginia
author_sort Kamerbeek, Constanza B.
title Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_short Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_full Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_fullStr Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_full_unstemmed Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_sort diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
publisher Elsevier
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/15214
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