Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection

Abstract: Assisted reproduction techniques (ARTs) have become widespread in the equine breeding industry. In particular, the combination of oocyte recovery from live mares followed by IVM and intracytoplasmic sperm injection (ICSI) has increased markedly among the ARTs used with valuable or low-fert...

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Autores principales: Rodríguez, María Belén, Gambini, Andrés, Clérico, Gabriel José, Ynsaurralde Rivolta, Amada Eugenia, Briski, Olinda, Largel, Hernan, Sansiñena, Marina Julia, Salamone, Daniel F.
Formato: Artículo
Lenguaje:Inglés
Publicado: CSIRO Publishing 2022
Materias:
CABALLOS
BLASTOCITOS
EMBRIONES
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14722
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14722
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic CABALLOS
BLASTOCITOS
EMBRIONES
spellingShingle CABALLOS
BLASTOCITOS
EMBRIONES
Rodríguez, María Belén
Gambini, Andrés
Clérico, Gabriel José
Ynsaurralde Rivolta, Amada Eugenia
Briski, Olinda
Largel, Hernan
Sansiñena, Marina Julia
Salamone, Daniel F.
Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
topic_facet CABALLOS
BLASTOCITOS
EMBRIONES
description Abstract: Assisted reproduction techniques (ARTs) have become widespread in the equine breeding industry. In particular, the combination of oocyte recovery from live mares followed by IVM and intracytoplasmic sperm injection (ICSI) has increased markedly among the ARTs used with valuable or low-fertility animals. There is currently no consensus among research groups regarding the optimal oocyte maturation period to produce high-quality embryos. In this study, we report the maturation dynamics of equine oocytes at different time points, from 20 to 40 h (Experiment 1). In addition, in Experiment 2, equine ICSI blastocysts were produced from oocytes that exhibited early (up to 24 h) or late (28–30 h) extrusion of the first polar body (PB). Blastocyst rates and diameter were recorded and embryo quality was assessed by analysing the number of apoptotic cells and Yes-associated protein 1 (YAP1) expression. By 20 h of IVM, 42% of oocytes were mature, and the remaining oocytes matured within the next 17 h of IVM. Although no differences were found in cell apoptosis or the number of YAP1-positive cells between groups exhibiting early and late PB extrusion, embryos from the early group (Group I) exhibited an improved total cell number and blastocyst rate compared to embryos from the late group (Group II) (18.60% vs 10.17% respectively).
format Artículo
author Rodríguez, María Belén
Gambini, Andrés
Clérico, Gabriel José
Ynsaurralde Rivolta, Amada Eugenia
Briski, Olinda
Largel, Hernan
Sansiñena, Marina Julia
Salamone, Daniel F.
author_facet Rodríguez, María Belén
Gambini, Andrés
Clérico, Gabriel José
Ynsaurralde Rivolta, Amada Eugenia
Briski, Olinda
Largel, Hernan
Sansiñena, Marina Julia
Salamone, Daniel F.
author_sort Rodríguez, María Belén
title Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
title_short Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
title_full Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
title_fullStr Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
title_full_unstemmed Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
title_sort time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
publisher CSIRO Publishing
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14722
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