CFTR modulates RPS27 gene expression using chloride anion as signaling effector

Abstract: In Cystic Fibrosis (CF), the impairment of the CFTR channel activity leads to a variety of alterations, including differential gene expression. However, the CFTR signaling mechanisms remain unclear. Recently, culturing IB3-1 CF cells under different intracellular Clconcentrations ([Cl-...

Descripción completa

Detalles Bibliográficos
Autores principales: Valdivieso, Ángel Gabriel, Mori, Consuelo, Clauzure, Mariángeles, Massip Copiz, María Macarena, Santa Coloma, Tomás Antonio
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
REGULADOR DE CONDUCTANCIA DE TRANSMEMBRANA DE FIBROSIS QUISTICA
PROTEINA RIBOSOMICA 40S S27
FIBROSIS QUISTICA
CLORURO
GENES
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14560
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14560
record_format dspace
spelling I33-R139-123456789-145602023-11-23T16:58:00Z CFTR modulates RPS27 gene expression using chloride anion as signaling effector Valdivieso, Ángel Gabriel Mori, Consuelo Clauzure, Mariángeles Massip Copiz, María Macarena Santa Coloma, Tomás Antonio REGULADOR DE CONDUCTANCIA DE TRANSMEMBRANA DE FIBROSIS QUISTICA PROTEINA RIBOSOMICA 40S S27 FIBROSIS QUISTICA CLORURO GENES Abstract: In Cystic Fibrosis (CF), the impairment of the CFTR channel activity leads to a variety of alterations, including differential gene expression. However, the CFTR signaling mechanisms remain unclear. Recently, culturing IB3-1 CF cells under different intracellular Clconcentrations ([Cl- ]i), we observed several Cl- -dependent genes and further characterized one of them as RPS27. Thus, we hypothesized that Cl- might act as a signaling effector for CFTR signaling. Here, to test this idea, we study RPS27 expression in T84 cells modulating the CFTR activity by using CFTR inhibitors. First, we observed that incubation of T84 cells with increasing concentrations of the CFTR inhibitors CFTR(inh)-172 or GlyH-101 determined a progressive increase in the relative [Cl- ]i (using the Cl- fluorescent probe SPQ). The [Cl- ]i rise was concomitant with a dose-dependent down-regulation of RPS27. These results imply that CFTR inhibition produce Cl- accumulation and that RPS27 expression can be modulated by CFTR inhibition. Therefore, Cl- behaves as a signaling effector for CFTR in the modulation of RPS27 expression. In addition, the IL-1β receptor antagonist IL1RN or the JNK inhibitor SP600125, both restored the down-regulation of RPS27 induced by CFTRinh-172, implying a role of autocrine IL-1β and JNK signaling downstream of Cl- in RPS27 modulation. 2022-07-26T11:54:08Z 2022-07-26T11:54:08Z 2017 Artículo Valdivieso, Á. et al. CFTR modulates RPS27 gene expression using chloride anion as signaling effector [en línea]. Postprint del artículo publicado en: Archives of Biochemistry and Biophysics. 2017 (633) . doi: https://doi.org/10.1016/j.abb.2017.09.014. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14560 1096-0384 (online) 0003-9861 https://repositorio.uca.edu.ar/handle/123456789/14560 10.1016/j.abb.2017.09.014 28941802 eng Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Elsevier Archives of Biochemistry and Biophysics Vol. 633, 2017
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic REGULADOR DE CONDUCTANCIA DE TRANSMEMBRANA DE FIBROSIS QUISTICA
PROTEINA RIBOSOMICA 40S S27
FIBROSIS QUISTICA
CLORURO
GENES
spellingShingle REGULADOR DE CONDUCTANCIA DE TRANSMEMBRANA DE FIBROSIS QUISTICA
PROTEINA RIBOSOMICA 40S S27
FIBROSIS QUISTICA
CLORURO
GENES
Valdivieso, Ángel Gabriel
Mori, Consuelo
Clauzure, Mariángeles
Massip Copiz, María Macarena
Santa Coloma, Tomás Antonio
CFTR modulates RPS27 gene expression using chloride anion as signaling effector
topic_facet REGULADOR DE CONDUCTANCIA DE TRANSMEMBRANA DE FIBROSIS QUISTICA
PROTEINA RIBOSOMICA 40S S27
FIBROSIS QUISTICA
CLORURO
GENES
description Abstract: In Cystic Fibrosis (CF), the impairment of the CFTR channel activity leads to a variety of alterations, including differential gene expression. However, the CFTR signaling mechanisms remain unclear. Recently, culturing IB3-1 CF cells under different intracellular Clconcentrations ([Cl- ]i), we observed several Cl- -dependent genes and further characterized one of them as RPS27. Thus, we hypothesized that Cl- might act as a signaling effector for CFTR signaling. Here, to test this idea, we study RPS27 expression in T84 cells modulating the CFTR activity by using CFTR inhibitors. First, we observed that incubation of T84 cells with increasing concentrations of the CFTR inhibitors CFTR(inh)-172 or GlyH-101 determined a progressive increase in the relative [Cl- ]i (using the Cl- fluorescent probe SPQ). The [Cl- ]i rise was concomitant with a dose-dependent down-regulation of RPS27. These results imply that CFTR inhibition produce Cl- accumulation and that RPS27 expression can be modulated by CFTR inhibition. Therefore, Cl- behaves as a signaling effector for CFTR in the modulation of RPS27 expression. In addition, the IL-1β receptor antagonist IL1RN or the JNK inhibitor SP600125, both restored the down-regulation of RPS27 induced by CFTRinh-172, implying a role of autocrine IL-1β and JNK signaling downstream of Cl- in RPS27 modulation.
format Artículo
author Valdivieso, Ángel Gabriel
Mori, Consuelo
Clauzure, Mariángeles
Massip Copiz, María Macarena
Santa Coloma, Tomás Antonio
author_facet Valdivieso, Ángel Gabriel
Mori, Consuelo
Clauzure, Mariángeles
Massip Copiz, María Macarena
Santa Coloma, Tomás Antonio
author_sort Valdivieso, Ángel Gabriel
title CFTR modulates RPS27 gene expression using chloride anion as signaling effector
title_short CFTR modulates RPS27 gene expression using chloride anion as signaling effector
title_full CFTR modulates RPS27 gene expression using chloride anion as signaling effector
title_fullStr CFTR modulates RPS27 gene expression using chloride anion as signaling effector
title_full_unstemmed CFTR modulates RPS27 gene expression using chloride anion as signaling effector
title_sort cftr modulates rps27 gene expression using chloride anion as signaling effector
publisher Elsevier
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14560
work_keys_str_mv AT valdiviesoangelgabriel cftrmodulatesrps27geneexpressionusingchlorideanionassignalingeffector
AT moriconsuelo cftrmodulatesrps27geneexpressionusingchlorideanionassignalingeffector
AT clauzuremariangeles cftrmodulatesrps27geneexpressionusingchlorideanionassignalingeffector
AT massipcopizmariamacarena cftrmodulatesrps27geneexpressionusingchlorideanionassignalingeffector
AT santacolomatomasantonio cftrmodulatesrps27geneexpressionusingchlorideanionassignalingeffector
_version_ 1807949131975491584

Ejemplares similares