Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma

Abstract: Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced sclerode...

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Autores principales: Crescenti, Ernesto, J. V., Medina, Vanina Araceli, Sambuco, Lorena, Cremaschi, Graciela A., Genaro, Ana María, Cricco, Graciela P., Martín, Gabriela A., Valli, Eduardo, Martinel Lamas, Diego José, Perazzo, Juan C., Rivera, Elena S., Bergoc, Rosa M.
Formato: Artículo
Lenguaje:Español
Publicado: Springer 2022
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Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14264
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id I33-R139-123456789-14264
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Español
topic ESCLERODERMIA SISTEMICA
ANTICUERPOS
ANTIOXIDANTES
MASTOCITOS
MANGANESIO
SELENIO
PIEL
ZINC
spellingShingle ESCLERODERMIA SISTEMICA
ANTICUERPOS
ANTIOXIDANTES
MASTOCITOS
MANGANESIO
SELENIO
PIEL
ZINC
Crescenti, Ernesto, J. V.
Medina, Vanina Araceli
Sambuco, Lorena
Cremaschi, Graciela A.
Genaro, Ana María
Cricco, Graciela P.
Martín, Gabriela A.
Valli, Eduardo
Martinel Lamas, Diego José
Perazzo, Juan C.
Rivera, Elena S.
Bergoc, Rosa M.
Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
topic_facet ESCLERODERMIA SISTEMICA
ANTICUERPOS
ANTIOXIDANTES
MASTOCITOS
MANGANESIO
SELENIO
PIEL
ZINC
description Abstract: Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM + O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and BLM + O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956 ± 59 μm, P < 0.01), collagen deposition, and mast cells infiltration (43.1 ± 1.0 vs. 102 ± 14.1 mast cells, P < 0.05). O-LM administration significantly blocked BLM-induced oxidative damage and the enhanced immunoreactive fibroblasts for α-smooth muscle actin while reduced BLM-induced autoantibodies that strongly react mainly with skin and spleen. O-LM significantly reduced BLM-induced scleroderma through the modulation of antioxidant and immunological pathways.
format Artículo
author Crescenti, Ernesto, J. V.
Medina, Vanina Araceli
Sambuco, Lorena
Cremaschi, Graciela A.
Genaro, Ana María
Cricco, Graciela P.
Martín, Gabriela A.
Valli, Eduardo
Martinel Lamas, Diego José
Perazzo, Juan C.
Rivera, Elena S.
Bergoc, Rosa M.
author_facet Crescenti, Ernesto, J. V.
Medina, Vanina Araceli
Sambuco, Lorena
Cremaschi, Graciela A.
Genaro, Ana María
Cricco, Graciela P.
Martín, Gabriela A.
Valli, Eduardo
Martinel Lamas, Diego José
Perazzo, Juan C.
Rivera, Elena S.
Bergoc, Rosa M.
author_sort Crescenti, Ernesto, J. V.
title Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_short Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_full Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_fullStr Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_full_unstemmed Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_sort effects of oligoelements se, zn, and mn plus lachesis muta venom in experimental scleroderma
publisher Springer
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14264
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