Genetic deletion of Galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice

Abstract: We studied the role of galectin-3 (Gal-3) in the expression of alternative activation markers (M2) on macrophage, cytokines, and fibrosis through the temporal evolution of healing, ventricular remodeling, and function after myocardial infarction (MI). C57BL/6J and Gal-3 knockout mice (Lgal...

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Autores principales: Cassaglia, Pablo, Penas, Federico, Betazza, Celeste, Fontana Estevez, Florencia, Miksztowicz, Verónica, Martinez Naya, Nadia Laura, Llamosas, María Clara, Noli Truant, Sofía, Wilensky, Luciana, Volberg, Verónica, Cevey, Ágata C., Touceda, Vanessa, Cicale, Eliana, Berg, Gabriela, Fernández, Marisa, Goren, Nora, Morales, Celina, González, Germán E.
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
HERIDAS
GALECTIN 3
MACROFAGOS
INFARTO DEL MIOCARDIO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14249
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14249
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic HERIDAS
GALECTIN 3
MACROFAGOS
INFARTO DEL MIOCARDIO
spellingShingle HERIDAS
GALECTIN 3
MACROFAGOS
INFARTO DEL MIOCARDIO
Cassaglia, Pablo
Penas, Federico
Betazza, Celeste
Fontana Estevez, Florencia
Miksztowicz, Verónica
Martinez Naya, Nadia Laura
Llamosas, María Clara
Noli Truant, Sofía
Wilensky, Luciana
Volberg, Verónica
Cevey, Ágata C.
Touceda, Vanessa
Cicale, Eliana
Berg, Gabriela
Fernández, Marisa
Goren, Nora
Morales, Celina
González, Germán E.
Genetic deletion of Galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice
topic_facet HERIDAS
GALECTIN 3
MACROFAGOS
INFARTO DEL MIOCARDIO
description Abstract: We studied the role of galectin-3 (Gal-3) in the expression of alternative activation markers (M2) on macrophage, cytokines, and fibrosis through the temporal evolution of healing, ventricular remodeling, and function after myocardial infarction (MI). C57BL/6J and Gal-3 knockout mice (Lgals3-/-) were subjected to permanent coronary ligation or sham. We studied i) mortality, ii) macrophage infiltration and expression of markers of alternative activation, iii) cytokine, iv) matrix metalloproteinase-2 activity, v) fibrosis, and vi) cardiac function and remodeling. At 1 week post-MI, lack of Gal-3 markedly attenuated F4/80+ macrophage infiltration and significantly increased the expression of Mrc1 and Chil1, markers of M2 macrophages at the MI zone. Levels of IL-10, IL-6, and matrix metalloproteinase-2 were significantly increased, whereas tumor necrosis factor-α, transforming growth factor-β, and fibrosis were remarkably attenuated at the infarct zone. In Gal-3 knockout mice, scar thinning ratio, expansion, and cardiac remodeling and function were severely affected from the onset of MI. At 4 weeks post-MI, the natural evolution of fibrosis in Gal-3 knockout mice was also affected. Our results suggest that Gal-3 is essential for wound healing because it regulates the dynamics of macrophage infiltration, proinflammatory and anti-inflammatory cytokine expression, and fibrosis along the temporal evolution of MI in mice. The deficit of Gal-3 affected the dynamics of wound healing, thus aggravating the evolution of remodeling and function.
format Artículo
author Cassaglia, Pablo
Penas, Federico
Betazza, Celeste
Fontana Estevez, Florencia
Miksztowicz, Verónica
Martinez Naya, Nadia Laura
Llamosas, María Clara
Noli Truant, Sofía
Wilensky, Luciana
Volberg, Verónica
Cevey, Ágata C.
Touceda, Vanessa
Cicale, Eliana
Berg, Gabriela
Fernández, Marisa
Goren, Nora
Morales, Celina
González, Germán E.
author_facet Cassaglia, Pablo
Penas, Federico
Betazza, Celeste
Fontana Estevez, Florencia
Miksztowicz, Verónica
Martinez Naya, Nadia Laura
Llamosas, María Clara
Noli Truant, Sofía
Wilensky, Luciana
Volberg, Verónica
Cevey, Ágata C.
Touceda, Vanessa
Cicale, Eliana
Berg, Gabriela
Fernández, Marisa
Goren, Nora
Morales, Celina
González, Germán E.
author_sort Cassaglia, Pablo
title Genetic deletion of Galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice
title_short Genetic deletion of Galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice
title_full Genetic deletion of Galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice
title_fullStr Genetic deletion of Galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice
title_full_unstemmed Genetic deletion of Galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice
title_sort genetic deletion of galectin-3 alters the temporal evolution of macrophage infiltration and healing affecting the cardiac remodeling and function after myocardial infarction in mice
publisher Elsevier
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14249
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