Regulation of the cellular redox state and the expression of DNA methyltransferase-1 in peripheral blood mononuclear cells from patients with Graves’ disease

Abstract: Background: Graves’ disease is an autoimmune disorder characterised by excessive production of thyroid hormones, which induces increased cellular metabolism in most tissues and increased production of reactive oxygen species (ROS). The aim of this work was to analyse the effect of ROS...

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Autores principales: Saban, Melina, Costilla, Melisa, Klecha, Alicia Juana, Di Cugno, Mariana, Curriá, Marina Inés, Cremaschi, Graciela A., Barreiro Arcos, María Laura
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
ENFERMEDAD DE GRAVES
CELULAS MONONUCLEARES DE SANGRE PERIFÉRICA
ESTRES OXIDATIVO
ENZIMAS ANTIOXIDANTES
ADN METILTRANSFERASA-1
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14017
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14017
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic ENFERMEDAD DE GRAVES
CELULAS MONONUCLEARES DE SANGRE PERIFÉRICA
ESTRES OXIDATIVO
ENZIMAS ANTIOXIDANTES
ADN METILTRANSFERASA-1
spellingShingle ENFERMEDAD DE GRAVES
CELULAS MONONUCLEARES DE SANGRE PERIFÉRICA
ESTRES OXIDATIVO
ENZIMAS ANTIOXIDANTES
ADN METILTRANSFERASA-1
Saban, Melina
Costilla, Melisa
Klecha, Alicia Juana
Di Cugno, Mariana
Curriá, Marina Inés
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
Regulation of the cellular redox state and the expression of DNA methyltransferase-1 in peripheral blood mononuclear cells from patients with Graves’ disease
topic_facet ENFERMEDAD DE GRAVES
CELULAS MONONUCLEARES DE SANGRE PERIFÉRICA
ESTRES OXIDATIVO
ENZIMAS ANTIOXIDANTES
ADN METILTRANSFERASA-1
description Abstract: Background: Graves’ disease is an autoimmune disorder characterised by excessive production of thyroid hormones, which induces increased cellular metabolism in most tissues and increased production of reactive oxygen species (ROS). The aim of this work was to analyse the effect of ROS on cell viability and the expression of catalase (CAT), glutathione peroxidase-1 (GPx1), superoxide dismutase (SOD-1) and DNA methyltransferase-1 (DNMT-1) in peripheral blood mononuclear cells (PBMC) from patients with newly diagnosed Graves’ disease or treated with methimazole. Patients and methods: For this study, women patients with newly diagnosed Graves’ disease (n = 18), treated with methimazole (n = 6) and healthy subjects (n = 15) were recruited. ROS were evaluated by flow cytometry, and the viability/apoptosis of PBMC was analysed by flow cytometry and fluorescence microscopy. Genomic expression of CAT, GPx-1, SOD-1 and DNMT-1 was quantified by real-time PCR. Results: We found high levels of ROS and increased expression of CAT, GPx-1, SOD-1 and DNMT-1 in PBMC from patients with newly diagnosed Graves’ disease. Methimazole treatment reversed these parameters. Cell viability was similar in all study groups. Conclusions: ROS induces the expression of CAT, GPx-1, and SOD-1. The activity of these enzymes may contribute to the protection of PBMC from the harmful effect of free radicals on cell viability. Increased expression of DNMT-1 may be associated with aberrant methylation patterns in immunoregulatory genes contributing to autoimmunity in Graves’ disease.
format Artículo
author Saban, Melina
Costilla, Melisa
Klecha, Alicia Juana
Di Cugno, Mariana
Curriá, Marina Inés
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
author_facet Saban, Melina
Costilla, Melisa
Klecha, Alicia Juana
Di Cugno, Mariana
Curriá, Marina Inés
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
author_sort Saban, Melina
title Regulation of the cellular redox state and the expression of DNA methyltransferase-1 in peripheral blood mononuclear cells from patients with Graves’ disease
title_short Regulation of the cellular redox state and the expression of DNA methyltransferase-1 in peripheral blood mononuclear cells from patients with Graves’ disease
title_full Regulation of the cellular redox state and the expression of DNA methyltransferase-1 in peripheral blood mononuclear cells from patients with Graves’ disease
title_fullStr Regulation of the cellular redox state and the expression of DNA methyltransferase-1 in peripheral blood mononuclear cells from patients with Graves’ disease
title_full_unstemmed Regulation of the cellular redox state and the expression of DNA methyltransferase-1 in peripheral blood mononuclear cells from patients with Graves’ disease
title_sort regulation of the cellular redox state and the expression of dna methyltransferase-1 in peripheral blood mononuclear cells from patients with graves’ disease
publisher Elsevier
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14017
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