Overlapping synthetic peptides as a tool to map protein-protein interactions ̶FSH as a model system of nonadditive interactions

Abstract: In earlier work, we used partially overlapped synthetic peptides as a tool to find regions of interaction between the human FSH hormone and its receptor, aiming to find possible antagonists or agonists. Years later, the FSH and FSH receptor 3D structures were reported by other laborato...

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Detalles Bibliográficos
Autor principal: Santa Coloma, Tomás Antonio
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
PEPTIDO AGONISTA
PEPTIDO ANTAGONISTA
PARADOJAS DE ENERGIA LIBRE
INTERACCIONES PROTEÍNA-PROTEÍNA
ANTAGONISTAS DE LOS RECEPTORES
PEPTIDOS SINTETICOS
HORMONAS GLICOPROTEICAS DE SUBUNIDAD ALFA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/13975
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-13975
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic PEPTIDO AGONISTA
PEPTIDO ANTAGONISTA
PARADOJAS DE ENERGIA LIBRE
INTERACCIONES PROTEÍNA-PROTEÍNA
ANTAGONISTAS DE LOS RECEPTORES
PEPTIDOS SINTETICOS
HORMONAS GLICOPROTEICAS DE SUBUNIDAD ALFA
spellingShingle PEPTIDO AGONISTA
PEPTIDO ANTAGONISTA
PARADOJAS DE ENERGIA LIBRE
INTERACCIONES PROTEÍNA-PROTEÍNA
ANTAGONISTAS DE LOS RECEPTORES
PEPTIDOS SINTETICOS
HORMONAS GLICOPROTEICAS DE SUBUNIDAD ALFA
Santa Coloma, Tomás Antonio
Overlapping synthetic peptides as a tool to map protein-protein interactions ̶FSH as a model system of nonadditive interactions
topic_facet PEPTIDO AGONISTA
PEPTIDO ANTAGONISTA
PARADOJAS DE ENERGIA LIBRE
INTERACCIONES PROTEÍNA-PROTEÍNA
ANTAGONISTAS DE LOS RECEPTORES
PEPTIDOS SINTETICOS
HORMONAS GLICOPROTEICAS DE SUBUNIDAD ALFA
description Abstract: In earlier work, we used partially overlapped synthetic peptides as a tool to find regions of interaction between the human FSH hormone and its receptor, aiming to find possible antagonists or agonists. Years later, the FSH and FSH receptor 3D structures were reported by other laboratories. The 3D results were in close agreement with the interacting regions predicted by using synthetic peptides. These earlier studies are reviewed here, and the predicted regions of interaction compared to the FSH and FSH receptor 3D structures to illustrate the usefulness of the synthetic peptide strategy to find binding regions. Different contact regions contribute multiplicatively to the high affinity of the entire ligand; thus, peptides covering a fraction of the anchor sites and with low free energy density cannot reach the affinity of the entire molecule. The earlier use of multiple linear regression to find the relevant predictors for effective binding, and a new way to estimate ΔG° and nonadditive interactions for the synthetic peptides in solution, by using the buried surface area (BSA), will be discussed.
format Artículo
author Santa Coloma, Tomás Antonio
author_facet Santa Coloma, Tomás Antonio
author_sort Santa Coloma, Tomás Antonio
title Overlapping synthetic peptides as a tool to map protein-protein interactions ̶FSH as a model system of nonadditive interactions
title_short Overlapping synthetic peptides as a tool to map protein-protein interactions ̶FSH as a model system of nonadditive interactions
title_full Overlapping synthetic peptides as a tool to map protein-protein interactions ̶FSH as a model system of nonadditive interactions
title_fullStr Overlapping synthetic peptides as a tool to map protein-protein interactions ̶FSH as a model system of nonadditive interactions
title_full_unstemmed Overlapping synthetic peptides as a tool to map protein-protein interactions ̶FSH as a model system of nonadditive interactions
title_sort overlapping synthetic peptides as a tool to map protein-protein interactions ̶fsh as a model system of nonadditive interactions
publisher Elsevier
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/13975
work_keys_str_mv AT santacolomatomasantonio overlappingsyntheticpeptidesasatooltomapproteinproteininteractionsfshasamodelsystemofnonadditiveinteractions
bdutipo_str Repositorios
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