Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line
Abstract: Most of the deaths from skin cancer are caused by melanoma, a malignancy in which STAT3 plays a crucial role. The inhibition of STAT3 is considered a potential target to induce cell death, tumor regression and metastasis inhibition. The objective of this work was to evaluate the activity...
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2021
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I33-R139-123456789-128612023-04-24T22:26:33Z Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line Martino, Renzo Barreiro Arcos, María Laura Peralta, Ignacio Marrassini, Carla Saint Martin, Elina Malén Cogoi, Laura Cremaschi, Graciela A. Alonso, María Rosario Anesini, Claudia MELANOMA FITOTERAPIA APOPTOSIS METASTASIS Abstract: Most of the deaths from skin cancer are caused by melanoma, a malignancy in which STAT3 plays a crucial role. The inhibition of STAT3 is considered a potential target to induce cell death, tumor regression and metastasis inhibition. The objective of this work was to evaluate the activity of the aqueous extract of Larrea divaricata (Aq), a fraction rich in polyphenols (EA),and the isolated compound quercetin-3-methyl ether (Q3ME) on B16F10 melanoma cells. The effects of Aq, EA and Q3ME were assessed on B16F10 cells by determining the proliferation, viability, apoptosis induction and the expression and phosphorylation of STAT3. The phytochemical composition of the extracts was determined by High Performance Liquid Chromatography. Aq, EA and Q3ME presented antiproliferative activity on B6F10 cells through p-STAT3 inhibition and early and late apoptosis induction (EC50 EA= ≤0.1 μg/ml; Aq= 316±30 μg/ml; Q3ME= <0.1 μg/ml). L. divaricata could be considered for the development of adjuvant phytotherapies in melanoma treatment. 2021-11-11T20:34:54Z 2021-11-11T20:34:54Z 2021 Artículo Martino, R., et al. Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line [en línea]. Natural Product Research, 2021. doi:10.1080/14786419.2021.1980789. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/12861 1478-6419 (impreso) 1478-6427 (online) https://repositorio.uca.edu.ar/handle/123456789/12861 10.1080/14786419.2021.1980789 eng Acceso abierto. 12 meses de embargo http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Taylor and Francis Natural Product Research, 2021 |
institution |
Universidad Católica Argentina |
institution_str |
I-33 |
repository_str |
R-139 |
collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
language |
Inglés |
topic |
MELANOMA FITOTERAPIA APOPTOSIS METASTASIS |
spellingShingle |
MELANOMA FITOTERAPIA APOPTOSIS METASTASIS Martino, Renzo Barreiro Arcos, María Laura Peralta, Ignacio Marrassini, Carla Saint Martin, Elina Malén Cogoi, Laura Cremaschi, Graciela A. Alonso, María Rosario Anesini, Claudia Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line |
topic_facet |
MELANOMA FITOTERAPIA APOPTOSIS METASTASIS |
description |
Abstract: Most of the deaths from skin cancer are caused by melanoma, a malignancy in which STAT3 plays
a crucial role. The inhibition of STAT3 is considered a potential target to induce cell death, tumor
regression and metastasis inhibition. The objective of this work was to evaluate the activity of the
aqueous extract of Larrea divaricata (Aq), a fraction rich in polyphenols (EA),and the isolated
compound quercetin-3-methyl ether (Q3ME) on B16F10 melanoma cells. The effects of Aq, EA
and Q3ME were assessed on B16F10 cells by determining the proliferation, viability, apoptosis
induction and the expression and phosphorylation of STAT3. The phytochemical composition of
the extracts was determined by High Performance Liquid Chromatography. Aq, EA and Q3ME
presented antiproliferative activity on B6F10 cells through p-STAT3 inhibition and early and late
apoptosis induction (EC50 EA= ≤0.1 μg/ml; Aq= 316±30 μg/ml; Q3ME= <0.1 μg/ml). L. divaricata
could be considered for the development of adjuvant phytotherapies in melanoma treatment. |
format |
Artículo |
author |
Martino, Renzo Barreiro Arcos, María Laura Peralta, Ignacio Marrassini, Carla Saint Martin, Elina Malén Cogoi, Laura Cremaschi, Graciela A. Alonso, María Rosario Anesini, Claudia |
author_facet |
Martino, Renzo Barreiro Arcos, María Laura Peralta, Ignacio Marrassini, Carla Saint Martin, Elina Malén Cogoi, Laura Cremaschi, Graciela A. Alonso, María Rosario Anesini, Claudia |
author_sort |
Martino, Renzo |
title |
Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line |
title_short |
Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line |
title_full |
Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line |
title_fullStr |
Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line |
title_full_unstemmed |
Antiproliferative activity of aqueous and polyphenol-rich extracts of Larrea divaricata Cav. upon a melanoma cell line |
title_sort |
antiproliferative activity of aqueous and polyphenol-rich extracts of larrea divaricata cav. upon a melanoma cell line |
publisher |
Taylor and Francis |
publishDate |
2021 |
url |
https://repositorio.uca.edu.ar/handle/123456789/12861 |
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