Transport of charged small molecules after electropermeabilization — drift and diffusion

"We provide a direct quantitative comparison of molecular transport of three similarly sized but chemically different fluorescent indicators of membrane permeabilization — two cationic molecules (YO-PRO-1 and propidium), and one anionic molecule (calcein). Our results show that the influx of YO...

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Detalles Bibliográficos
Autores principales: Sözer, Esin B., Pocetti, Florencia, Vernier, P. Thomas
Formato: Artículos de Publicaciones Periódicas publishedVersion
Lenguaje:Inglés
Publicado: 2019
Materias:
MEMBRANAS
MOLECULAS
TRANSPORTE
CELULAS
Acceso en línea:http://ri.itba.edu.ar/handle/123456789/1582
Aporte de:
Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA) de Instituto Tecnológico de Buenos Aires (ITBA)
id I32-R138-123456789-1582
record_format dspace
spelling I32-R138-123456789-15822022-12-07T13:06:57Z Transport of charged small molecules after electropermeabilization — drift and diffusion Sözer, Esin B. Pocetti, Florencia Vernier, P. Thomas MEMBRANAS MOLECULAS TRANSPORTE CELULAS "We provide a direct quantitative comparison of molecular transport of three similarly sized but chemically different fluorescent indicators of membrane permeabilization — two cationic molecules (YO-PRO-1 and propidium), and one anionic molecule (calcein). Our results show that the influx of YO-PRO-1 and propidium (both cations) into electropermeabilized cells is an order of magnitude greater than that of calcein (an anion) after the same pulse exposure. Calcein efflux from cells loaded before permeabilization, however, is similar in magnitude to YO-PRO-1 and propidium influx." 2019-05-20T19:12:24Z 2019-05-20T19:12:24Z 2018-03 Artículos de Publicaciones Periódicas info:eu-repo/semantics/publishedVersion 2046-1682 http://ri.itba.edu.ar/handle/123456789/1582 en info:eu-repo/grantAgreement/AFOSR/MURI/FA9550-15-1-0517/US. Arlington, VA info:eu-repo/grantAgreement/AFOSR/FA9550–14–1-0123/US. Arlington, VA info:eu-repo/semantics/reference/doi.org/10.1186/s13628-018-0044-2 info:eu-repo/grantAgreement/MECCyT/BEC.AR/AR.Ciudad Autónoma de Buenos Aires http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf
institution Instituto Tecnológico de Buenos Aires (ITBA)
institution_str I-32
repository_str R-138
collection Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA)
language Inglés
topic MEMBRANAS
MOLECULAS
TRANSPORTE
CELULAS
spellingShingle MEMBRANAS
MOLECULAS
TRANSPORTE
CELULAS
Sözer, Esin B.
Pocetti, Florencia
Vernier, P. Thomas
Transport of charged small molecules after electropermeabilization — drift and diffusion
topic_facet MEMBRANAS
MOLECULAS
TRANSPORTE
CELULAS
description "We provide a direct quantitative comparison of molecular transport of three similarly sized but chemically different fluorescent indicators of membrane permeabilization — two cationic molecules (YO-PRO-1 and propidium), and one anionic molecule (calcein). Our results show that the influx of YO-PRO-1 and propidium (both cations) into electropermeabilized cells is an order of magnitude greater than that of calcein (an anion) after the same pulse exposure. Calcein efflux from cells loaded before permeabilization, however, is similar in magnitude to YO-PRO-1 and propidium influx."
format Artículos de Publicaciones Periódicas
publishedVersion
author Sözer, Esin B.
Pocetti, Florencia
Vernier, P. Thomas
author_facet Sözer, Esin B.
Pocetti, Florencia
Vernier, P. Thomas
author_sort Sözer, Esin B.
title Transport of charged small molecules after electropermeabilization — drift and diffusion
title_short Transport of charged small molecules after electropermeabilization — drift and diffusion
title_full Transport of charged small molecules after electropermeabilization — drift and diffusion
title_fullStr Transport of charged small molecules after electropermeabilization — drift and diffusion
title_full_unstemmed Transport of charged small molecules after electropermeabilization — drift and diffusion
title_sort transport of charged small molecules after electropermeabilization — drift and diffusion
publishDate 2019
url http://ri.itba.edu.ar/handle/123456789/1582
work_keys_str_mv AT sozeresinb transportofchargedsmallmoleculesafterelectropermeabilizationdriftanddiffusion
AT pocettiflorencia transportofchargedsmallmoleculesafterelectropermeabilizationdriftanddiffusion
AT vernierpthomas transportofchargedsmallmoleculesafterelectropermeabilizationdriftanddiffusion
_version_ 1765660967059849216

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