Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides

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Breast cancer represents the most common kind of cancer in women and it is estimatedthat more than one million new cases are diagnosed each year worldwide. It is a complexdisease, which presents a variety of subgroups with different genetic and histopathologicfeatures and therefore with different re...

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Detalles Bibliográficos
Autor principal: Díaz Bessone, María Inés
Otros Autores: Urtreger, Alejandro Jorge
Formato: Tesis doctoral publishedVersion
Lenguaje:Español
Publicado: Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales 2016
Materias:
CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
Acceso en línea:https://hdl.handle.net/20.500.12110/tesis_n6034_DiazBessone
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=aextesis&d=tesis_n6034_DiazBessone_oai
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-tesis_n6034_DiazBessone_oai
record_format dspace
spelling I28-R145-tesis_n6034_DiazBessone_oai2024-09-02 Urtreger, Alejandro Jorge Díaz Bessone, María Inés 2016-03-28 Breast cancer represents the most common kind of cancer in women and it is estimatedthat more than one million new cases are diagnosed each year worldwide. It is a complexdisease, which presents a variety of subgroups with different genetic and histopathologicfeatures and therefore with different responses to treatments. Multiple signaling pathways are involved in malignant progression. Some of them arepromising targets for therapeutic intervention, including protein kinase C (PKC), involvedin cellular events such as proliferation, differentiation and apoptosis and the retinoidsystem, widely implicated in cell differentiation. Since different mammary malignancies exhibit differential expression of PKC isoforms, wehave developed (human and murine) mammary cell models overexpressing α or δ PKCisoforms. Using these models we studied how PKCα or PKCδ alters cell parametersassociated with tumor progression and metastatic dissemination while we assessed theresponse to treatment with retinoids (ATRA). Our results suggest that PKCα overexpression confers to the cells a retinoic acid-sensitivephenotype, through an arrest in the G0 / G1 phase of the cell cycle. Overexpression of PKCδ, in MDA-MB231 cells, induced a less aggressive phenotype, significantly reducing itsinvasive capability. Finally the lack of response of this cell line to ATRA treatment could bedue to the inability to trans-repress AP-1 sites. Fil: Díaz Bessone, María Inés. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf https://hdl.handle.net/20.500.12110/tesis_n6034_DiazBessone spa Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar CANCER DE MAMA PROTEINA QUINASA C ACIDO RETINOICO BREAST CANCER PROTEIN KINASE C RETINOIC ACID Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides Effect of overexpression of different isoforms of Protein Kinase C (PKC) in modulation of malignant phenotype of mammary ephitelial cells. Alteration in sensitivity to treatment with retinoids info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=aextesis&d=tesis_n6034_DiazBessone_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
spellingShingle CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
Díaz Bessone, María Inés
Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
topic_facet CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
description Breast cancer represents the most common kind of cancer in women and it is estimatedthat more than one million new cases are diagnosed each year worldwide. It is a complexdisease, which presents a variety of subgroups with different genetic and histopathologicfeatures and therefore with different responses to treatments. Multiple signaling pathways are involved in malignant progression. Some of them arepromising targets for therapeutic intervention, including protein kinase C (PKC), involvedin cellular events such as proliferation, differentiation and apoptosis and the retinoidsystem, widely implicated in cell differentiation. Since different mammary malignancies exhibit differential expression of PKC isoforms, wehave developed (human and murine) mammary cell models overexpressing α or δ PKCisoforms. Using these models we studied how PKCα or PKCδ alters cell parametersassociated with tumor progression and metastatic dissemination while we assessed theresponse to treatment with retinoids (ATRA). Our results suggest that PKCα overexpression confers to the cells a retinoic acid-sensitivephenotype, through an arrest in the G0 / G1 phase of the cell cycle. Overexpression of PKCδ, in MDA-MB231 cells, induced a less aggressive phenotype, significantly reducing itsinvasive capability. Finally the lack of response of this cell line to ATRA treatment could bedue to the inability to trans-repress AP-1 sites.
author2 Urtreger, Alejandro Jorge
author_facet Urtreger, Alejandro Jorge
Díaz Bessone, María Inés
format Tesis doctoral
Tesis doctoral
publishedVersion
author Díaz Bessone, María Inés
author_sort Díaz Bessone, María Inés
title Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_short Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_full Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_fullStr Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_full_unstemmed Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_sort efecto de la sobreexpresión de distintas isoformas de la proteína quinasa c (pkc) en la modulación del fenotipo maligno de células mamarias epiteliales. alteración en la sensibilidad al tratamiento con retinoides
publisher Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales
publishDate 2016
url https://hdl.handle.net/20.500.12110/tesis_n6034_DiazBessone
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=aextesis&d=tesis_n6034_DiazBessone_oai
work_keys_str_mv AT diazbessonemariaines efectodelasobreexpresiondedistintasisoformasdelaproteinaquinasacpkcenlamodulaciondelfenotipomalignodecelulasmamariasepitelialesalteracionenlasensibilidadaltratamientoconretinoides
AT diazbessonemariaines effectofoverexpressionofdifferentisoformsofproteinkinasecpkcinmodulationofmalignantphenotypeofmammaryephitelialcellsalterationinsensitivitytotreatmentwithretinoids
_version_ 1824356218850770944

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