Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation

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The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male...

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Autores principales:	Sanchez, M.C., Alvarez Sedo, C., Julianelli, V.L., Romanato, M., Calvo, L., Calvo, J.C., Fontana, V.A.
Formato:	Artículo publishedVersion
Publicado:	2013
Materias:	Chromatin decondensation Dermatan sulfate Heparin Murine spermatozoa Synergism dermatan sulfate glutathione glycosaminoglycan heparin animal cell article chromatin chromatin decondensation' controlled study dose response in vitro study male mouse nonhuman phase contrast microscopy priority journal spermatozoon transmission electron microscopy Animals Chromatin Dermatan Sulfate Dose-Response Relationship, Drug Male Mice Microscopy, Electron Spermatozoa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19396368_v59_n2_p82_Sanchez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_19396368_v59_n2_p82_Sanchez_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_19396368_v59_n2_p82_Sanchez_oai
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spelling	I28-R145-paper_19396368_v59_n2_p82_Sanchez_oai2024-08-16 Sanchez, M.C. Alvarez Sedo, C. Julianelli, V.L. Romanato, M. Calvo, L. Calvo, J.C. Fontana, V.A. 2013 The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male pronucleus and syngamy. Decondensation is accomplished by protamine disulfide bond reduction by oocyte glutathione and replacement of protamines by oocyte histones with the aid of an acceptor molecule. Previous results from our laboratory have demonstrated that heparan sulfate (HS) present in the ooplasm functions as protamine acceptor during human sperm decondensation in vivo. In the present paper, we analyze the role of heparin, structural analogue of HS, and dermatan sulfate (DS) in murine sperm chromatin decondensation in vitro, including the possibility of a synergistic effect between both glycosaminoglycans. Decondensation was assessed under phase contrast microscopy following incubation of murine spermatozoa with glutathione and either heparin, DS, or a combination of both. Ultrastructural changes taking place during decondensation were analyzed by transmission electron microscopy. Both glycosaminoglycans were able to promote the decondensation of murine spermatozoa in vitro but the decondensing ability of heparin was significantly higher. Use of both glycosaminoglycans together revealed the existence of a synergistic effect. Transmission electron microscopy analysis of decondensing spermatozoa supported these findings. Synergism between heparin and DS was observed both in capacitated and non-capacitated spermatozoa but decondensation kinetics was faster in the former. The results obtained indicate a new potential role for dermatan sulfate in murine sperm decondensation at fertilization and provide evidence of differences in the degree of chromatin condensation throughout the murine sperm nucleus. © 2013 Informa Healthcare USA, Inc. Fil:Julianelli, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romanato, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fontana, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_19396368_v59_n2_p82_Sanchez info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Syst. Biology Reprod. Med. 2013;59(2):82-90 Chromatin decondensation Dermatan sulfate Heparin Murine spermatozoa Synergism dermatan sulfate glutathione glycosaminoglycan heparin animal cell article chromatin chromatin decondensation' controlled study dose response in vitro study male mouse nonhuman phase contrast microscopy priority journal spermatozoon transmission electron microscopy Animals Chromatin Dermatan Sulfate Dose-Response Relationship, Drug Heparin Male Mice Microscopy, Electron Spermatozoa Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_19396368_v59_n2_p82_Sanchez_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Chromatin decondensation Dermatan sulfate Heparin Murine spermatozoa Synergism dermatan sulfate glutathione glycosaminoglycan heparin animal cell article chromatin chromatin decondensation' controlled study dose response in vitro study male mouse nonhuman phase contrast microscopy priority journal spermatozoon transmission electron microscopy Animals Chromatin Dermatan Sulfate Dose-Response Relationship, Drug Heparin Male Mice Microscopy, Electron Spermatozoa
spellingShingle	Chromatin decondensation Dermatan sulfate Heparin Murine spermatozoa Synergism dermatan sulfate glutathione glycosaminoglycan heparin animal cell article chromatin chromatin decondensation' controlled study dose response in vitro study male mouse nonhuman phase contrast microscopy priority journal spermatozoon transmission electron microscopy Animals Chromatin Dermatan Sulfate Dose-Response Relationship, Drug Heparin Male Mice Microscopy, Electron Spermatozoa Sanchez, M.C. Alvarez Sedo, C. Julianelli, V.L. Romanato, M. Calvo, L. Calvo, J.C. Fontana, V.A. Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
topic_facet	Chromatin decondensation Dermatan sulfate Heparin Murine spermatozoa Synergism dermatan sulfate glutathione glycosaminoglycan heparin animal cell article chromatin chromatin decondensation' controlled study dose response in vitro study male mouse nonhuman phase contrast microscopy priority journal spermatozoon transmission electron microscopy Animals Chromatin Dermatan Sulfate Dose-Response Relationship, Drug Heparin Male Mice Microscopy, Electron Spermatozoa
description	The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male pronucleus and syngamy. Decondensation is accomplished by protamine disulfide bond reduction by oocyte glutathione and replacement of protamines by oocyte histones with the aid of an acceptor molecule. Previous results from our laboratory have demonstrated that heparan sulfate (HS) present in the ooplasm functions as protamine acceptor during human sperm decondensation in vivo. In the present paper, we analyze the role of heparin, structural analogue of HS, and dermatan sulfate (DS) in murine sperm chromatin decondensation in vitro, including the possibility of a synergistic effect between both glycosaminoglycans. Decondensation was assessed under phase contrast microscopy following incubation of murine spermatozoa with glutathione and either heparin, DS, or a combination of both. Ultrastructural changes taking place during decondensation were analyzed by transmission electron microscopy. Both glycosaminoglycans were able to promote the decondensation of murine spermatozoa in vitro but the decondensing ability of heparin was significantly higher. Use of both glycosaminoglycans together revealed the existence of a synergistic effect. Transmission electron microscopy analysis of decondensing spermatozoa supported these findings. Synergism between heparin and DS was observed both in capacitated and non-capacitated spermatozoa but decondensation kinetics was faster in the former. The results obtained indicate a new potential role for dermatan sulfate in murine sperm decondensation at fertilization and provide evidence of differences in the degree of chromatin condensation throughout the murine sperm nucleus. © 2013 Informa Healthcare USA, Inc.
format	Artículo Artículo publishedVersion
author	Sanchez, M.C. Alvarez Sedo, C. Julianelli, V.L. Romanato, M. Calvo, L. Calvo, J.C. Fontana, V.A.
author_facet	Sanchez, M.C. Alvarez Sedo, C. Julianelli, V.L. Romanato, M. Calvo, L. Calvo, J.C. Fontana, V.A.
author_sort	Sanchez, M.C.
title	Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_short	Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_full	Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_fullStr	Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_full_unstemmed	Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_sort	dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
publishDate	2013
url	http://hdl.handle.net/20.500.12110/paper_19396368_v59_n2_p82_Sanchez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_19396368_v59_n2_p82_Sanchez_oai
work_keys_str_mv	AT sanchezmc dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation AT alvarezsedoc dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation AT julianellivl dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation AT romanatom dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation AT calvol dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation AT calvojc dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation AT fontanava dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
_version_	1809356848176824320

Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation

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