Analysis of persistence during intracellular actin-based transport mediated by molecular motors
The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the mot...
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| Autores principales: | , , , |
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| Formato: | Documento de conferencia publishedVersion |
| Publicado: |
2010
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_17426588_v246_n_p_Pallavicini_oai |
| Aporte de: |
| Sumario: | The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd. |
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