Analysis of persistence during intracellular actin-based transport mediated by molecular motors

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The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the mot...

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Autores principales:	Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L.
Formato:	Documento de conferencia publishedVersion
Publicado:	2010
Materias:	Cytology Anomalous diffusion Cell cytoplasm Function of time Molecular motors Spatio-temporal resolution Tracking techniques Turning angles Xenopus laevis Proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_17426588_v246_n_p_Pallavicini_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_17426588_v246_n_p_Pallavicini_oai
record_format	dspace
spelling	I28-R145-paper_17426588_v246_n_p_Pallavicini_oai2024-08-16 Pallavicini, C. Despósito, M.A. Levi, V. Bruno, L. 2010 The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd. Fil:Despósito, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Phys. Conf. Ser. 2010;246 Cytology Anomalous diffusion Cell cytoplasm Function of time Molecular motors Spatio-temporal resolution Tracking techniques Turning angles Xenopus laevis Proteins Analysis of persistence during intracellular actin-based transport mediated by molecular motors info:eu-repo/semantics/conferenceObject info:ar-repo/semantics/documento de conferencia info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_17426588_v246_n_p_Pallavicini_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Cytology Anomalous diffusion Cell cytoplasm Function of time Molecular motors Spatio-temporal resolution Tracking techniques Turning angles Xenopus laevis Proteins
spellingShingle	Cytology Anomalous diffusion Cell cytoplasm Function of time Molecular motors Spatio-temporal resolution Tracking techniques Turning angles Xenopus laevis Proteins Pallavicini, C. Despósito, M.A. Levi, V. Bruno, L. Analysis of persistence during intracellular actin-based transport mediated by molecular motors
topic_facet	Cytology Anomalous diffusion Cell cytoplasm Function of time Molecular motors Spatio-temporal resolution Tracking techniques Turning angles Xenopus laevis Proteins
description	The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.
format	Documento de conferencia Documento de conferencia publishedVersion
author	Pallavicini, C. Despósito, M.A. Levi, V. Bruno, L.
author_facet	Pallavicini, C. Despósito, M.A. Levi, V. Bruno, L.
author_sort	Pallavicini, C.
title	Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_short	Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_full	Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_fullStr	Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_full_unstemmed	Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_sort	analysis of persistence during intracellular actin-based transport mediated by molecular motors
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_17426588_v246_n_p_Pallavicini_oai
work_keys_str_mv	AT pallavicinic analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors AT despositoma analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors AT leviv analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors AT brunol analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors
_version_	1809356931736797184

Analysis of persistence during intracellular actin-based transport mediated by molecular motors

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