Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B

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Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell respon...

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Autores principales:	Giudice, J., Barcos, L.S., Guaimas, F.F., Penas-Steinhardt, A., Giordano, L., Jares-Erijman, E.A., Coluccio Leskow, F.
Formato:	Artículo publishedVersion
Publicado:	2013
Materias:	Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1478811X_v11_n1_p_Giudice http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_1478811X_v11_n1_p_Giudice_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_1478811X_v11_n1_p_Giudice_oai
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spelling	I28-R145-paper_1478811X_v11_n1_p_Giudice_oai2020-10-19 Giudice, J. Barcos, L.S. Guaimas, F.F. Penas-Steinhardt, A. Giordano, L. Jares-Erijman, E.A. Coluccio Leskow, F. 2013 Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses. Results: We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. We combined biotinylated ligands with streptavidin conjugated quantum dots and visible fluorescent proteins to visualize the binding of IGF-II and insulin to IR-B and their ensuing internalization. By confocal microscopy and flow cytometry in living cells, we studied the internalization kinetic through the IR-B of both IGF-II, known to elicit proliferative responses, and insulin, a regulator of metabolism. Conclusions: IGF-II promotes a faster internalization of IR-B than insulin. We propose that IGF-II differentially activates mitogenic responses through endosomes, while insulin-activated IR-B remains at the plasma membrane. This fact could facilitate the interaction with key effector molecules involved in metabolism regulation. © 2013 Giudice et al.; licensee BioMed Central Ltd. Fil:Giudice, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giordano, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coluccio Leskow, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_1478811X_v11_n1_p_Giudice info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Cell Commun. Signal. 2013;11(1) Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_1478811X_v11_n1_p_Giudice_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction
spellingShingle	Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction Giudice, J. Barcos, L.S. Guaimas, F.F. Penas-Steinhardt, A. Giordano, L. Jares-Erijman, E.A. Coluccio Leskow, F. Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
topic_facet	Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction
description	Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses. Results: We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. We combined biotinylated ligands with streptavidin conjugated quantum dots and visible fluorescent proteins to visualize the binding of IGF-II and insulin to IR-B and their ensuing internalization. By confocal microscopy and flow cytometry in living cells, we studied the internalization kinetic through the IR-B of both IGF-II, known to elicit proliferative responses, and insulin, a regulator of metabolism. Conclusions: IGF-II promotes a faster internalization of IR-B than insulin. We propose that IGF-II differentially activates mitogenic responses through endosomes, while insulin-activated IR-B remains at the plasma membrane. This fact could facilitate the interaction with key effector molecules involved in metabolism regulation. © 2013 Giudice et al.; licensee BioMed Central Ltd.
format	Artículo Artículo publishedVersion
author	Giudice, J. Barcos, L.S. Guaimas, F.F. Penas-Steinhardt, A. Giordano, L. Jares-Erijman, E.A. Coluccio Leskow, F.
author_facet	Giudice, J. Barcos, L.S. Guaimas, F.F. Penas-Steinhardt, A. Giordano, L. Jares-Erijman, E.A. Coluccio Leskow, F.
author_sort	Giudice, J.
title	Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
title_short	Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
title_full	Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
title_fullStr	Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
title_full_unstemmed	Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
title_sort	insulin and insulin like growth factor ii endocytosis and signaling via insulin receptor b
publishDate	2013
url	http://hdl.handle.net/20.500.12110/paper_1478811X_v11_n1_p_Giudice http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_1478811X_v11_n1_p_Giudice_oai
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_version_	1766026777554059264

Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B

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