Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene
Background: Eukaryotic DNA methylation is one of the most studied epigenetic processes, as it results in a direct and heritable covalent modification triggered by external stimuli. In contrast to mammals, plant DNA methylation, which is stimulated by external cues exemplified by various abiotic type...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | Artículo publishedVersion |
| Publicado: |
2011
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14712229_v11_n_p_Gonzalez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_14712229_v11_n_p_Gonzalez_oai |
| Aporte de: |
| id |
I28-R145-paper_14712229_v11_n_p_Gonzalez_oai |
|---|---|
| record_format |
dspace |
| spelling |
I28-R145-paper_14712229_v11_n_p_Gonzalez_oai2024-08-16 González, R.M. Ricardi, M.M. Iusem, N.D. 2011 Background: Eukaryotic DNA methylation is one of the most studied epigenetic processes, as it results in a direct and heritable covalent modification triggered by external stimuli. In contrast to mammals, plant DNA methylation, which is stimulated by external cues exemplified by various abiotic types of stress, is often found not only at CG sites but also at CNG (N denoting A, C or T) and CNN (asymmetric) sites. A genome-wide analysis of DNA methylation in Arabidopsis has shown that CNN methylation is preferentially concentrated in transposon genes and non-coding repetitive elements. We are particularly interested in investigating the epigenetics of plant species with larger and more complex genomes than Arabidopsis, particularly with regards to the associated alterations elicited by abiotic stress.Results: We describe the existence of CNN-methylated epialleles that span Asr1, a non-transposon, protein-coding gene from tomato plants that lacks an orthologous counterpart in Arabidopsis. In addition, to test the hypothesis of a link between epigenetics modifications and the adaptation of crop plants to abiotic stress, we exhaustively explored the cytosine methylation status in leaf Asr1 DNA, a model gene in our system, resulting from water-deficit stress conditions imposed on tomato plants. We found that drought conditions brought about removal of methyl marks at approximately 75 of the 110 asymmetric (CNN) sites analysed, concomitantly with a decrease of the repressive H3K27me3 epigenetic mark and a large induction of expression at the RNA level. When pinpointing those sites, we observed that demethylation occurred mostly in the intronic region.Conclusions: These results demonstrate a novel genomic distribution of CNN methylation, namely in the transcribed region of a protein-coding, non-repetitive gene, and the changes in those epigenetic marks that are caused by water stress. These findings may represent a general mechanism for the acquisition of new epialleles in somatic cells, which are pivotal for regulating gene expression in plants. © 2011 González et al; licensee BioMed Central Ltd. Fil:González, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ricardi, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_14712229_v11_n_p_Gonzalez info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar BMC Plant Biol. 2011;11 Epigeneticsasymmetric methylationAsr1water stress Tomato Arabidopsis Eukaryota Lycopersicon esculentum Mammalia Asr1 protein, Lycopersicon esculentum cytosine plant DNA vegetable protein article biosynthesis dehydration DNA methylation gene expression regulation genetic epigenesis genetics metabolism methylation tomato Cytosine Dehydration DNA Methylation DNA, Plant Epigenesis, Genetic Gene Expression Regulation, Plant Lycopersicon esculentum Methylation Plant Proteins Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_14712229_v11_n_p_Gonzalez_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Epigeneticsasymmetric methylationAsr1water stress Tomato Arabidopsis Eukaryota Lycopersicon esculentum Mammalia Asr1 protein, Lycopersicon esculentum cytosine plant DNA vegetable protein article biosynthesis dehydration DNA methylation gene expression regulation genetic epigenesis genetics metabolism methylation tomato Cytosine Dehydration DNA Methylation DNA, Plant Epigenesis, Genetic Gene Expression Regulation, Plant Lycopersicon esculentum Methylation Plant Proteins |
| spellingShingle |
Epigeneticsasymmetric methylationAsr1water stress Tomato Arabidopsis Eukaryota Lycopersicon esculentum Mammalia Asr1 protein, Lycopersicon esculentum cytosine plant DNA vegetable protein article biosynthesis dehydration DNA methylation gene expression regulation genetic epigenesis genetics metabolism methylation tomato Cytosine Dehydration DNA Methylation DNA, Plant Epigenesis, Genetic Gene Expression Regulation, Plant Lycopersicon esculentum Methylation Plant Proteins González, R.M. Ricardi, M.M. Iusem, N.D. Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene |
| topic_facet |
Epigeneticsasymmetric methylationAsr1water stress Tomato Arabidopsis Eukaryota Lycopersicon esculentum Mammalia Asr1 protein, Lycopersicon esculentum cytosine plant DNA vegetable protein article biosynthesis dehydration DNA methylation gene expression regulation genetic epigenesis genetics metabolism methylation tomato Cytosine Dehydration DNA Methylation DNA, Plant Epigenesis, Genetic Gene Expression Regulation, Plant Lycopersicon esculentum Methylation Plant Proteins |
| description |
Background: Eukaryotic DNA methylation is one of the most studied epigenetic processes, as it results in a direct and heritable covalent modification triggered by external stimuli. In contrast to mammals, plant DNA methylation, which is stimulated by external cues exemplified by various abiotic types of stress, is often found not only at CG sites but also at CNG (N denoting A, C or T) and CNN (asymmetric) sites. A genome-wide analysis of DNA methylation in Arabidopsis has shown that CNN methylation is preferentially concentrated in transposon genes and non-coding repetitive elements. We are particularly interested in investigating the epigenetics of plant species with larger and more complex genomes than Arabidopsis, particularly with regards to the associated alterations elicited by abiotic stress.Results: We describe the existence of CNN-methylated epialleles that span Asr1, a non-transposon, protein-coding gene from tomato plants that lacks an orthologous counterpart in Arabidopsis. In addition, to test the hypothesis of a link between epigenetics modifications and the adaptation of crop plants to abiotic stress, we exhaustively explored the cytosine methylation status in leaf Asr1 DNA, a model gene in our system, resulting from water-deficit stress conditions imposed on tomato plants. We found that drought conditions brought about removal of methyl marks at approximately 75 of the 110 asymmetric (CNN) sites analysed, concomitantly with a decrease of the repressive H3K27me3 epigenetic mark and a large induction of expression at the RNA level. When pinpointing those sites, we observed that demethylation occurred mostly in the intronic region.Conclusions: These results demonstrate a novel genomic distribution of CNN methylation, namely in the transcribed region of a protein-coding, non-repetitive gene, and the changes in those epigenetic marks that are caused by water stress. These findings may represent a general mechanism for the acquisition of new epialleles in somatic cells, which are pivotal for regulating gene expression in plants. © 2011 González et al; licensee BioMed Central Ltd. |
| format |
Artículo Artículo publishedVersion |
| author |
González, R.M. Ricardi, M.M. Iusem, N.D. |
| author_facet |
González, R.M. Ricardi, M.M. Iusem, N.D. |
| author_sort |
González, R.M. |
| title |
Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene |
| title_short |
Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene |
| title_full |
Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene |
| title_fullStr |
Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene |
| title_full_unstemmed |
Atypical epigenetic mark in an atypical location: Cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene |
| title_sort |
atypical epigenetic mark in an atypical location: cytosine methylation at asymmetric (cnn) sites within the body of a non-repetitive tomato gene |
| publishDate |
2011 |
| url |
http://hdl.handle.net/20.500.12110/paper_14712229_v11_n_p_Gonzalez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_14712229_v11_n_p_Gonzalez_oai |
| work_keys_str_mv |
AT gonzalezrm atypicalepigeneticmarkinanatypicallocationcytosinemethylationatasymmetriccnnsiteswithinthebodyofanonrepetitivetomatogene AT ricardimm atypicalepigeneticmarkinanatypicallocationcytosinemethylationatasymmetriccnnsiteswithinthebodyofanonrepetitivetomatogene AT iusemnd atypicalepigeneticmarkinanatypicallocationcytosinemethylationatasymmetriccnnsiteswithinthebodyofanonrepetitivetomatogene |
| _version_ |
1809356926961582080 |