Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract

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Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), an...

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Autores principales:	Gholipour, Y., Nonami, H., Erra-Balsells, R.
Formato:	Artículo publishedVersion
Publicado:	2008
Materias:	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v19_n12_p1841_Gholipour_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_10440305_v19_n12_p1841_Gholipour_oai
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spelling	I28-R145-paper_10440305_v19_n12_p1841_Gholipour_oai2024-08-16 Gholipour, Y. Nonami, H. Erra-Balsells, R. 2008 Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Am. Soc. Mass Spectrom. 2008;19(12):1841-1848 Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v19_n12_p1841_Gholipour_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa
spellingShingle	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa Gholipour, Y. Nonami, H. Erra-Balsells, R. Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
topic_facet	Carbohydrates Carbon nanotubes Cells Cytology Glucose Military engineering Organic compounds Polysaccharides Positive ions Potential flow Sugar (sucrose) Sugars Carbohydrate contents Cell cytoplasms Dihydroxybenzoic acids Direct analyses High resolutions Leaf cells Microcapillary tips Positive ion modes Pressure probes Reproducibility Trihydroxyacetophenone Biomolecules 2,4,6 trihydroxyacetophenone acetophenone derivative carbon nanotube cell extract disaccharide gentisic acid hexose unclassified drug article carbohydrate analysis cytoplasm matrix assisted laser desorption ionization time of flight mass spectrometry plant cell plant leaf Carbohydrates Cytoplasm Plants Pressure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tulipa Ultraviolet Rays Tulipa
description	Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry.
format	Artículo Artículo publishedVersion
author	Gholipour, Y. Nonami, H. Erra-Balsells, R.
author_facet	Gholipour, Y. Nonami, H. Erra-Balsells, R.
author_sort	Gholipour, Y.
title	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_short	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_full	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_fullStr	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_full_unstemmed	Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
title_sort	application of pressure probe and uv-maldi-tof ms for direct analysis of plant underivatized carbohydrates in subpicoliter single-cell cytoplasm extract
publishDate	2008
url	http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v19_n12_p1841_Gholipour_oai
work_keys_str_mv	AT gholipoury applicationofpressureprobeanduvmalditofmsfordirectanalysisofplantunderivatizedcarbohydratesinsubpicolitersinglecellcytoplasmextract AT nonamih applicationofpressureprobeanduvmalditofmsfordirectanalysisofplantunderivatizedcarbohydratesinsubpicolitersinglecellcytoplasmextract AT errabalsellsr applicationofpressureprobeanduvmalditofmsfordirectanalysisofplantunderivatizedcarbohydratesinsubpicolitersinglecellcytoplasmextract
_version_	1809357106530222080

Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract

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