Galectin-1: Biphasic growth regulation of Leydig tumor cells

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Galectin-1 (Gal-1) is a widely expressed β-galactoside-binding protein that exerts pleiotropic biological functions. To gain insight into the potential role of Gal-1 as a novel modulator of Leydig cells, we investigated its effect on the growth and death of MA-10 tumor Leydig cells. In this study, w...

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Detalles Bibliográficos
Autores principales: Biron, V.A., Iglesias, M., Troncoso, M.F., Besio-Moreno, M., Patrignani, Z.J., Pignataro, O.P., Wolfenstein-Todel, C.
Formato: Artículo publishedVersion
Publicado: 2006
Materias:
Apoptosis
Galectin-1
Leydig cells
Proliferation
caspase 3
caspase 8
caspase 8 inhibitor
caspase 9
caspase 9 inhibitor
cytochrome c
death receptor
disaccharide
DNA fragment
FAS ligand
galectin 1
lactose
animal cell
apoptosis
article
carbohydrate analysis
cell growth
cell membrane potential
cell proliferation
controlled study
cytofluorometry
enzyme activation
growth regulation
Leydig cell
Leydig cell tumor
male
mitochondrial membrane
mouse
nonhuman
pathophysiology
priority journal
protein determination
protein expression
protein function
protein secretion
Cell Line, Tumor
Cell Proliferation
Cytoplasm
DNA Fragmentation
Dose-Response Relationship, Drug
Galectin 1
Gene Expression Regulation, Neoplastic
Humans
Lactose
Leydig Cells
Male
Membrane Potentials
Mitochondria
Neoplasm Proteins
Sertoli-Leydig Cell Tumor
Testicular Neoplasms
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09596658_v16_n9_p810_Biron
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_09596658_v16_n9_p810_Biron_oai
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Galectin-1 (Gal-1) is a widely expressed β-galactoside-binding protein that exerts pleiotropic biological functions. To gain insight into the potential role of Gal-1 as a novel modulator of Leydig cells, we investigated its effect on the growth and death of MA-10 tumor Leydig cells. In this study, we identified cytoplasmic Gal-1 expression in these tumor cells by cytofluorometry. DNA fragmentation, caspase-3, -8, and -9 activation, loss of mitochondrial membrane potential (ΔΨ m), cytochrome c (Cyt c) release, and FasL expression suggested that relatively high concentrations of exogenously added recombinant Gal-1 (rGal-1) induced apoptosis by the mitochondrial and death receptor pathways. These pathways were independently activated, as the presence of the inhibitor of caspase-8 or -9 only partially prevented Gal-1-effect. On the contrary, low concentrations of Gal-1 significantly promoted cell proliferation, without inducing cell death. Importantly, the presence of the disaccharide lactose prevented Gal-1 effects, suggesting the involvement of the carbohydrate recognition domain (CRD). This study provides strong evidence that Gal-1 is a novel biphasic regulator of Leydig tumor cell number, suggesting a novel role for Gal-1 in the reproductive physiopathology. © Copyright 2006 Oxford University Press.

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