Galectin-1: Biphasic growth regulation of Leydig tumor cells

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Galectin-1 (Gal-1) is a widely expressed β-galactoside-binding protein that exerts pleiotropic biological functions. To gain insight into the potential role of Gal-1 as a novel modulator of Leydig cells, we investigated its effect on the growth and death of MA-10 tumor Leydig cells. In this study, w...

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Autores principales:	Biron, V.A., Iglesias, M., Troncoso, M.F., Besio-Moreno, M., Patrignani, Z.J., Pignataro, O.P., Wolfenstein-Todel, C.
Formato:	Artículo publishedVersion
Publicado:	2006
Materias:	Apoptosis Galectin-1 Leydig cells Proliferation caspase 3 caspase 8 caspase 8 inhibitor caspase 9 caspase 9 inhibitor cytochrome c death receptor disaccharide DNA fragment FAS ligand galectin 1 lactose animal cell apoptosis article carbohydrate analysis cell growth cell membrane potential cell proliferation controlled study cytofluorometry enzyme activation growth regulation Leydig cell Leydig cell tumor male mitochondrial membrane mouse nonhuman pathophysiology priority journal protein determination protein expression protein function protein secretion Cell Line, Tumor Cell Proliferation Cytoplasm DNA Fragmentation Dose-Response Relationship, Drug Galectin 1 Gene Expression Regulation, Neoplastic Humans Lactose Leydig Cells Male Membrane Potentials Mitochondria Neoplasm Proteins Sertoli-Leydig Cell Tumor Testicular Neoplasms
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09596658_v16_n9_p810_Biron http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_09596658_v16_n9_p810_Biron_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-paper_09596658_v16_n9_p810_Biron_oai2020-10-19 Biron, V.A. Iglesias, M. Troncoso, M.F. Besio-Moreno, M. Patrignani, Z.J. Pignataro, O.P. Wolfenstein-Todel, C. 2006 Galectin-1 (Gal-1) is a widely expressed β-galactoside-binding protein that exerts pleiotropic biological functions. To gain insight into the potential role of Gal-1 as a novel modulator of Leydig cells, we investigated its effect on the growth and death of MA-10 tumor Leydig cells. In this study, we identified cytoplasmic Gal-1 expression in these tumor cells by cytofluorometry. DNA fragmentation, caspase-3, -8, and -9 activation, loss of mitochondrial membrane potential (ΔΨ m), cytochrome c (Cyt c) release, and FasL expression suggested that relatively high concentrations of exogenously added recombinant Gal-1 (rGal-1) induced apoptosis by the mitochondrial and death receptor pathways. These pathways were independently activated, as the presence of the inhibitor of caspase-8 or -9 only partially prevented Gal-1-effect. On the contrary, low concentrations of Gal-1 significantly promoted cell proliferation, without inducing cell death. Importantly, the presence of the disaccharide lactose prevented Gal-1 effects, suggesting the involvement of the carbohydrate recognition domain (CRD). This study provides strong evidence that Gal-1 is a novel biphasic regulator of Leydig tumor cell number, suggesting a novel role for Gal-1 in the reproductive physiopathology. © Copyright 2006 Oxford University Press. Fil:Troncoso, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Patrignani, Z.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_09596658_v16_n9_p810_Biron info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Glycobiology 2006;16(9):810-821 Apoptosis Galectin-1 Leydig cells Proliferation caspase 3 caspase 8 caspase 8 inhibitor caspase 9 caspase 9 inhibitor cytochrome c death receptor disaccharide DNA fragment FAS ligand galectin 1 lactose animal cell apoptosis article carbohydrate analysis cell growth cell membrane potential cell proliferation controlled study cytofluorometry enzyme activation growth regulation Leydig cell Leydig cell tumor male mitochondrial membrane mouse nonhuman pathophysiology priority journal protein determination protein expression protein function protein secretion Cell Line, Tumor Cell Proliferation Cytoplasm DNA Fragmentation Dose-Response Relationship, Drug Galectin 1 Gene Expression Regulation, Neoplastic Humans Lactose Leydig Cells Male Membrane Potentials Mitochondria Neoplasm Proteins Sertoli-Leydig Cell Tumor Testicular Neoplasms Galectin-1: Biphasic growth regulation of Leydig tumor cells info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_09596658_v16_n9_p810_Biron_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Apoptosis Galectin-1 Leydig cells Proliferation caspase 3 caspase 8 caspase 8 inhibitor caspase 9 caspase 9 inhibitor cytochrome c death receptor disaccharide DNA fragment FAS ligand galectin 1 lactose animal cell apoptosis article carbohydrate analysis cell growth cell membrane potential cell proliferation controlled study cytofluorometry enzyme activation growth regulation Leydig cell Leydig cell tumor male mitochondrial membrane mouse nonhuman pathophysiology priority journal protein determination protein expression protein function protein secretion Cell Line, Tumor Cell Proliferation Cytoplasm DNA Fragmentation Dose-Response Relationship, Drug Galectin 1 Gene Expression Regulation, Neoplastic Humans Lactose Leydig Cells Male Membrane Potentials Mitochondria Neoplasm Proteins Sertoli-Leydig Cell Tumor Testicular Neoplasms
spellingShingle	Apoptosis Galectin-1 Leydig cells Proliferation caspase 3 caspase 8 caspase 8 inhibitor caspase 9 caspase 9 inhibitor cytochrome c death receptor disaccharide DNA fragment FAS ligand galectin 1 lactose animal cell apoptosis article carbohydrate analysis cell growth cell membrane potential cell proliferation controlled study cytofluorometry enzyme activation growth regulation Leydig cell Leydig cell tumor male mitochondrial membrane mouse nonhuman pathophysiology priority journal protein determination protein expression protein function protein secretion Cell Line, Tumor Cell Proliferation Cytoplasm DNA Fragmentation Dose-Response Relationship, Drug Galectin 1 Gene Expression Regulation, Neoplastic Humans Lactose Leydig Cells Male Membrane Potentials Mitochondria Neoplasm Proteins Sertoli-Leydig Cell Tumor Testicular Neoplasms Biron, V.A. Iglesias, M. Troncoso, M.F. Besio-Moreno, M. Patrignani, Z.J. Pignataro, O.P. Wolfenstein-Todel, C. Galectin-1: Biphasic growth regulation of Leydig tumor cells
topic_facet	Apoptosis Galectin-1 Leydig cells Proliferation caspase 3 caspase 8 caspase 8 inhibitor caspase 9 caspase 9 inhibitor cytochrome c death receptor disaccharide DNA fragment FAS ligand galectin 1 lactose animal cell apoptosis article carbohydrate analysis cell growth cell membrane potential cell proliferation controlled study cytofluorometry enzyme activation growth regulation Leydig cell Leydig cell tumor male mitochondrial membrane mouse nonhuman pathophysiology priority journal protein determination protein expression protein function protein secretion Cell Line, Tumor Cell Proliferation Cytoplasm DNA Fragmentation Dose-Response Relationship, Drug Galectin 1 Gene Expression Regulation, Neoplastic Humans Lactose Leydig Cells Male Membrane Potentials Mitochondria Neoplasm Proteins Sertoli-Leydig Cell Tumor Testicular Neoplasms
description	Galectin-1 (Gal-1) is a widely expressed β-galactoside-binding protein that exerts pleiotropic biological functions. To gain insight into the potential role of Gal-1 as a novel modulator of Leydig cells, we investigated its effect on the growth and death of MA-10 tumor Leydig cells. In this study, we identified cytoplasmic Gal-1 expression in these tumor cells by cytofluorometry. DNA fragmentation, caspase-3, -8, and -9 activation, loss of mitochondrial membrane potential (ΔΨ m), cytochrome c (Cyt c) release, and FasL expression suggested that relatively high concentrations of exogenously added recombinant Gal-1 (rGal-1) induced apoptosis by the mitochondrial and death receptor pathways. These pathways were independently activated, as the presence of the inhibitor of caspase-8 or -9 only partially prevented Gal-1-effect. On the contrary, low concentrations of Gal-1 significantly promoted cell proliferation, without inducing cell death. Importantly, the presence of the disaccharide lactose prevented Gal-1 effects, suggesting the involvement of the carbohydrate recognition domain (CRD). This study provides strong evidence that Gal-1 is a novel biphasic regulator of Leydig tumor cell number, suggesting a novel role for Gal-1 in the reproductive physiopathology. © Copyright 2006 Oxford University Press.
format	Artículo Artículo publishedVersion
author	Biron, V.A. Iglesias, M. Troncoso, M.F. Besio-Moreno, M. Patrignani, Z.J. Pignataro, O.P. Wolfenstein-Todel, C.
author_facet	Biron, V.A. Iglesias, M. Troncoso, M.F. Besio-Moreno, M. Patrignani, Z.J. Pignataro, O.P. Wolfenstein-Todel, C.
author_sort	Biron, V.A.
title	Galectin-1: Biphasic growth regulation of Leydig tumor cells
title_short	Galectin-1: Biphasic growth regulation of Leydig tumor cells
title_full	Galectin-1: Biphasic growth regulation of Leydig tumor cells
title_fullStr	Galectin-1: Biphasic growth regulation of Leydig tumor cells
title_full_unstemmed	Galectin-1: Biphasic growth regulation of Leydig tumor cells
title_sort	galectin-1: biphasic growth regulation of leydig tumor cells
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_09596658_v16_n9_p810_Biron http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_09596658_v16_n9_p810_Biron_oai
work_keys_str_mv	AT bironva galectin1biphasicgrowthregulationofleydigtumorcells AT iglesiasm galectin1biphasicgrowthregulationofleydigtumorcells AT troncosomf galectin1biphasicgrowthregulationofleydigtumorcells AT besiomorenom galectin1biphasicgrowthregulationofleydigtumorcells AT patrignanizj galectin1biphasicgrowthregulationofleydigtumorcells AT pignataroop galectin1biphasicgrowthregulationofleydigtumorcells AT wolfensteintodelc galectin1biphasicgrowthregulationofleydigtumorcells
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Galectin-1: Biphasic growth regulation of Leydig tumor cells

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