Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts

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Trypanosoma cruzi, the aetiological agent of Chagas' disease, is exposed to extremely different environment conditions during its life cycle, and transporters are key molecules for its adaptive regulation. Amino acids, and particularly arginine, are essential components in T. cruzi metabolism....

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Autores principales:	Carrillo, C., Canepa, G.E., Giacometti, A., Bouvier, L.A., Miranda, M.R., De Los Milagros Camara, M., Pereira, C.A.
Formato:	Artículo publishedVersion
Publicado:	2010
Materias:	AAAP family Amino acid transport Arginine permease Chagas' disease Trypanosoma cruzi arginine arginine permease auxin permease bacterial enzyme canavanine unclassified drug active transport amino acid transport amino terminal sequence article bacterial strain binding affinity carboxy terminal sequence controlled study expression vector gene identification genetic analysis nonhuman parasite identification parasite migration priority journal sensitivity and specificity transport kinetics yeast Amino Acid Transport Systems, Basic Arginine Genetic Complementation Test Protozoan Proteins Recombinant Proteins Saccharomyces cerevisiae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03781097_v306_n2_p97_Carrillo http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v306_n2_p97_Carrillo_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_03781097_v306_n2_p97_Carrillo_oai
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spelling	I28-R145-paper_03781097_v306_n2_p97_Carrillo_oai2020-10-19 Carrillo, C. Canepa, G.E. Giacometti, A. Bouvier, L.A. Miranda, M.R. De Los Milagros Camara, M. Pereira, C.A. 2010 Trypanosoma cruzi, the aetiological agent of Chagas' disease, is exposed to extremely different environment conditions during its life cycle, and transporters are key molecules for its adaptive regulation. Amino acids, and particularly arginine, are essential components in T. cruzi metabolism. In this work, a novel T. cruzi arginine permease was identified by screening different members of the AAAP family (amino acid/auxin permeases) in yeast complementation assays using a toxic arginine analogue. One gene candidate, TcAAAP411, was characterized as a very specific, high-affinity, l-arginine permease. This work is the first identification of the molecular components involved specifically in amino acid transport in T. cruzi and provides new insights for further validation of the TcAAAP family as functional permeases. © 2010 Federation of European Microbiological Societies. Fil:Carrillo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Canepa, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bouvier, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Los Milagros Camara, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereira, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_03781097_v306_n2_p97_Carrillo info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar FEMS Microbiol. Lett. 2010;306(2):97-102 AAAP family Amino acid transport Arginine permease Chagas' disease Trypanosoma cruzi arginine arginine permease auxin permease bacterial enzyme canavanine unclassified drug active transport amino acid transport amino terminal sequence article bacterial strain binding affinity carboxy terminal sequence controlled study expression vector gene identification genetic analysis nonhuman parasite identification parasite migration priority journal sensitivity and specificity transport kinetics Trypanosoma cruzi yeast Amino Acid Transport Systems, Basic Arginine Genetic Complementation Test Protozoan Proteins Recombinant Proteins Saccharomyces cerevisiae Trypanosoma cruzi Trypanosoma cruzi Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v306_n2_p97_Carrillo_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	AAAP family Amino acid transport Arginine permease Chagas' disease Trypanosoma cruzi arginine arginine permease auxin permease bacterial enzyme canavanine unclassified drug active transport amino acid transport amino terminal sequence article bacterial strain binding affinity carboxy terminal sequence controlled study expression vector gene identification genetic analysis nonhuman parasite identification parasite migration priority journal sensitivity and specificity transport kinetics Trypanosoma cruzi yeast Amino Acid Transport Systems, Basic Arginine Genetic Complementation Test Protozoan Proteins Recombinant Proteins Saccharomyces cerevisiae Trypanosoma cruzi Trypanosoma cruzi
spellingShingle	AAAP family Amino acid transport Arginine permease Chagas' disease Trypanosoma cruzi arginine arginine permease auxin permease bacterial enzyme canavanine unclassified drug active transport amino acid transport amino terminal sequence article bacterial strain binding affinity carboxy terminal sequence controlled study expression vector gene identification genetic analysis nonhuman parasite identification parasite migration priority journal sensitivity and specificity transport kinetics Trypanosoma cruzi yeast Amino Acid Transport Systems, Basic Arginine Genetic Complementation Test Protozoan Proteins Recombinant Proteins Saccharomyces cerevisiae Trypanosoma cruzi Trypanosoma cruzi Carrillo, C. Canepa, G.E. Giacometti, A. Bouvier, L.A. Miranda, M.R. De Los Milagros Camara, M. Pereira, C.A. Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
topic_facet	AAAP family Amino acid transport Arginine permease Chagas' disease Trypanosoma cruzi arginine arginine permease auxin permease bacterial enzyme canavanine unclassified drug active transport amino acid transport amino terminal sequence article bacterial strain binding affinity carboxy terminal sequence controlled study expression vector gene identification genetic analysis nonhuman parasite identification parasite migration priority journal sensitivity and specificity transport kinetics Trypanosoma cruzi yeast Amino Acid Transport Systems, Basic Arginine Genetic Complementation Test Protozoan Proteins Recombinant Proteins Saccharomyces cerevisiae Trypanosoma cruzi Trypanosoma cruzi
description	Trypanosoma cruzi, the aetiological agent of Chagas' disease, is exposed to extremely different environment conditions during its life cycle, and transporters are key molecules for its adaptive regulation. Amino acids, and particularly arginine, are essential components in T. cruzi metabolism. In this work, a novel T. cruzi arginine permease was identified by screening different members of the AAAP family (amino acid/auxin permeases) in yeast complementation assays using a toxic arginine analogue. One gene candidate, TcAAAP411, was characterized as a very specific, high-affinity, l-arginine permease. This work is the first identification of the molecular components involved specifically in amino acid transport in T. cruzi and provides new insights for further validation of the TcAAAP family as functional permeases. © 2010 Federation of European Microbiological Societies.
format	Artículo Artículo publishedVersion
author	Carrillo, C. Canepa, G.E. Giacometti, A. Bouvier, L.A. Miranda, M.R. De Los Milagros Camara, M. Pereira, C.A.
author_facet	Carrillo, C. Canepa, G.E. Giacometti, A. Bouvier, L.A. Miranda, M.R. De Los Milagros Camara, M. Pereira, C.A.
author_sort	Carrillo, C.
title	Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
title_short	Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
title_full	Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
title_fullStr	Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
title_full_unstemmed	Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
title_sort	trypanosoma cruzi amino acid transporter tcaaap411 mediates arginine uptake in yeasts
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_03781097_v306_n2_p97_Carrillo http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v306_n2_p97_Carrillo_oai
work_keys_str_mv	AT carrilloc trypanosomacruziaminoacidtransportertcaaap411mediatesarginineuptakeinyeasts AT canepage trypanosomacruziaminoacidtransportertcaaap411mediatesarginineuptakeinyeasts AT giacomettia trypanosomacruziaminoacidtransportertcaaap411mediatesarginineuptakeinyeasts AT bouvierla trypanosomacruziaminoacidtransportertcaaap411mediatesarginineuptakeinyeasts AT mirandamr trypanosomacruziaminoacidtransportertcaaap411mediatesarginineuptakeinyeasts AT delosmilagroscamaram trypanosomacruziaminoacidtransportertcaaap411mediatesarginineuptakeinyeasts AT pereiraca trypanosomacruziaminoacidtransportertcaaap411mediatesarginineuptakeinyeasts
_version_	1766026706167005184

Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts

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