A plasmid vector for isolation of strong promoters in Escherichia coli
In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning...
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1999
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v177_n1_p75_Carbonelli_oai |
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I28-R145-paper_03781097_v177_n1_p75_Carbonelli_oai2020-10-19 Carbonelli, D.L. Corley, E. Seigelchifer, M. Zorzópulos, J. 1999 In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies. Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar FEMS Microbiol. Lett. 1999;177(1):75-82 Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage A plasmid vector for isolation of strong promoters in Escherichia coli info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v177_n1_p75_Carbonelli_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage |
| spellingShingle |
Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage Carbonelli, D.L. Corley, E. Seigelchifer, M. Zorzópulos, J. A plasmid vector for isolation of strong promoters in Escherichia coli |
| topic_facet |
Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage |
| description |
In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies. |
| format |
Artículo Artículo publishedVersion |
| author |
Carbonelli, D.L. Corley, E. Seigelchifer, M. Zorzópulos, J. |
| author_facet |
Carbonelli, D.L. Corley, E. Seigelchifer, M. Zorzópulos, J. |
| author_sort |
Carbonelli, D.L. |
| title |
A plasmid vector for isolation of strong promoters in Escherichia coli |
| title_short |
A plasmid vector for isolation of strong promoters in Escherichia coli |
| title_full |
A plasmid vector for isolation of strong promoters in Escherichia coli |
| title_fullStr |
A plasmid vector for isolation of strong promoters in Escherichia coli |
| title_full_unstemmed |
A plasmid vector for isolation of strong promoters in Escherichia coli |
| title_sort |
plasmid vector for isolation of strong promoters in escherichia coli |
| publishDate |
1999 |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v177_n1_p75_Carbonelli_oai |
| work_keys_str_mv |
AT carbonellidl aplasmidvectorforisolationofstrongpromotersinescherichiacoli AT corleye aplasmidvectorforisolationofstrongpromotersinescherichiacoli AT seigelchiferm aplasmidvectorforisolationofstrongpromotersinescherichiacoli AT zorzopulosj aplasmidvectorforisolationofstrongpromotersinescherichiacoli AT carbonellidl plasmidvectorforisolationofstrongpromotersinescherichiacoli AT corleye plasmidvectorforisolationofstrongpromotersinescherichiacoli AT seigelchiferm plasmidvectorforisolationofstrongpromotersinescherichiacoli AT zorzopulosj plasmidvectorforisolationofstrongpromotersinescherichiacoli |
| _version_ |
1766026704446291968 |