Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)

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UDP-Glucose:glycoprotein glucosyltransferase (UGGT) is a central component of the endoplasmic reticulum (ER) glycoprotein-folding quality control system, which prevents the exit of partially folded species. UGGT activity can be regulated by the accumulation of misfolded proteins in the ER, a stimulu...

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Autores principales:	Prados, M.B., Caramelo, J.J., Miranda, S.E.
Formato:	Artículo publishedVersion
Publicado:	2013
Materias:	DJN Endoplasmic reticulum quality control Folding P4 Progesterone UDP-Glc: glycoprotein glucosyltransferase UGGT UPR glucosyltransferase glycoprotein progesterone UDP glucose glycoprotein glucosyltransferase unclassified drug animal experiment animal model article cell proliferation cellular distribution controlled study endoplasmic reticulum enzyme activity gene expression gene silencing hormonal regulation hybridoma hybridoma cell culture intracellular signaling mouse nonhuman priority journal protein analysis protein expression protein folding protein function protein synthesis quality control unfolded protein response
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01674889_v1833_n12_p3368_Prados http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01674889_v1833_n12_p3368_Prados_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_01674889_v1833_n12_p3368_Prados_oai
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spelling	I28-R145-paper_01674889_v1833_n12_p3368_Prados_oai2020-10-19 Prados, M.B. Caramelo, J.J. Miranda, S.E. 2013 UDP-Glucose:glycoprotein glucosyltransferase (UGGT) is a central component of the endoplasmic reticulum (ER) glycoprotein-folding quality control system, which prevents the exit of partially folded species. UGGT activity can be regulated by the accumulation of misfolded proteins in the ER, a stimulus that triggers a complex signaling pathway known as unfolded protein response (UPR) which is closely associated with inflammation and disease. In this work, we investigated the effect of progesterone (P4) on the expression and activity of UGGT in a mouse hybridoma. We detected the expression of two UGGT isoforms, UGGT1 and UGGT2, and demonstrated that both isoforms are active in these cells. Interestingly, the expression of each isoform is regulated by high physiological P4 concentrations. This work provides the first evidence of a hormonal regulation of UGGT isoform expression and activity, which might influence the glycoprotein quality control mechanism. These findings could contribute to the study of pathologies triggered by the accumulation of misfolded proteins. © 2013 Elsevier B.V. application/pdf http://hdl.handle.net/20.500.12110/paper_01674889_v1833_n12_p3368_Prados info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Biochim. Biophys. Acta Mol. Cell Res. 2013;1833(12):3368-3374 DJN Endoplasmic reticulum quality control Folding P4 Progesterone UDP-Glc: glycoprotein glucosyltransferase UGGT UPR glucosyltransferase glycoprotein progesterone UDP glucose glycoprotein glucosyltransferase unclassified drug animal experiment animal model article cell proliferation cellular distribution controlled study endoplasmic reticulum enzyme activity gene expression gene silencing hormonal regulation hybridoma hybridoma cell culture intracellular signaling mouse nonhuman priority journal protein analysis protein expression protein folding protein function protein synthesis quality control unfolded protein response Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT) info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01674889_v1833_n12_p3368_Prados_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	DJN Endoplasmic reticulum quality control Folding P4 Progesterone UDP-Glc: glycoprotein glucosyltransferase UGGT UPR glucosyltransferase glycoprotein progesterone UDP glucose glycoprotein glucosyltransferase unclassified drug animal experiment animal model article cell proliferation cellular distribution controlled study endoplasmic reticulum enzyme activity gene expression gene silencing hormonal regulation hybridoma hybridoma cell culture intracellular signaling mouse nonhuman priority journal protein analysis protein expression protein folding protein function protein synthesis quality control unfolded protein response
spellingShingle	DJN Endoplasmic reticulum quality control Folding P4 Progesterone UDP-Glc: glycoprotein glucosyltransferase UGGT UPR glucosyltransferase glycoprotein progesterone UDP glucose glycoprotein glucosyltransferase unclassified drug animal experiment animal model article cell proliferation cellular distribution controlled study endoplasmic reticulum enzyme activity gene expression gene silencing hormonal regulation hybridoma hybridoma cell culture intracellular signaling mouse nonhuman priority journal protein analysis protein expression protein folding protein function protein synthesis quality control unfolded protein response Prados, M.B. Caramelo, J.J. Miranda, S.E. Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)
topic_facet	DJN Endoplasmic reticulum quality control Folding P4 Progesterone UDP-Glc: glycoprotein glucosyltransferase UGGT UPR glucosyltransferase glycoprotein progesterone UDP glucose glycoprotein glucosyltransferase unclassified drug animal experiment animal model article cell proliferation cellular distribution controlled study endoplasmic reticulum enzyme activity gene expression gene silencing hormonal regulation hybridoma hybridoma cell culture intracellular signaling mouse nonhuman priority journal protein analysis protein expression protein folding protein function protein synthesis quality control unfolded protein response
description	UDP-Glucose:glycoprotein glucosyltransferase (UGGT) is a central component of the endoplasmic reticulum (ER) glycoprotein-folding quality control system, which prevents the exit of partially folded species. UGGT activity can be regulated by the accumulation of misfolded proteins in the ER, a stimulus that triggers a complex signaling pathway known as unfolded protein response (UPR) which is closely associated with inflammation and disease. In this work, we investigated the effect of progesterone (P4) on the expression and activity of UGGT in a mouse hybridoma. We detected the expression of two UGGT isoforms, UGGT1 and UGGT2, and demonstrated that both isoforms are active in these cells. Interestingly, the expression of each isoform is regulated by high physiological P4 concentrations. This work provides the first evidence of a hormonal regulation of UGGT isoform expression and activity, which might influence the glycoprotein quality control mechanism. These findings could contribute to the study of pathologies triggered by the accumulation of misfolded proteins. © 2013 Elsevier B.V.
format	Artículo Artículo publishedVersion
author	Prados, M.B. Caramelo, J.J. Miranda, S.E.
author_facet	Prados, M.B. Caramelo, J.J. Miranda, S.E.
author_sort	Prados, M.B.
title	Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)
title_short	Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)
title_full	Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)
title_fullStr	Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)
title_full_unstemmed	Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)
title_sort	progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor udp-glc: glycoprotein glucosyltransferase (uggt)
publishDate	2013
url	http://hdl.handle.net/20.500.12110/paper_01674889_v1833_n12_p3368_Prados http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01674889_v1833_n12_p3368_Prados_oai
work_keys_str_mv	AT pradosmb progesteroneregulatestheexpressionandactivityoftwomouseisoformsoftheglycoproteinfoldingsensorudpglcglycoproteinglucosyltransferaseuggt AT caramelojj progesteroneregulatestheexpressionandactivityoftwomouseisoformsoftheglycoproteinfoldingsensorudpglcglycoproteinglucosyltransferaseuggt AT mirandase progesteroneregulatestheexpressionandactivityoftwomouseisoformsoftheglycoproteinfoldingsensorudpglcglycoproteinglucosyltransferaseuggt
_version_	1766026675742572544

Progesterone regulates the expression and activity of two mouse isoforms of the glycoprotein folding sensor UDP-Glc: Glycoprotein glucosyltransferase (UGGT)

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