Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The p...
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2006
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01674889_v1763_n2_p238_Pregi_oai |
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I28-R145-paper_01674889_v1763_n2_p238_Pregi_oai2024-08-16 Pregi, N. Vittori, D. Pérez, G. Leirós, C.P. Nesse, A. 2006 Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved. Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Biochim. Biophys. Acta Mol. Cell Res. 2006;1763(2):238-246 Apoptosis Bcl-X Erythropoietin Neuroprotection SH-SY5Y cell Staurosporine erythropoietin protein bcl xl staurosporine apoptosis article cell death cell differentiation cell growth cell structure controlled study human human cell immunoprecipitation neurite neuroblastoma cell priority journal reverse transcription polymerase chain reaction Western blotting Apoptosis bcl-X Protein Blotting, Western Cell Differentiation Dose-Response Relationship, Drug Electrophoresis Enzyme Inhibitors Erythropoietin Erythropoietin, Recombinant Humans Kinetics Microscopy, Fluorescence Neuroblastoma Precipitin Tests Receptors, Erythropoietin Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Staurosporine Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01674889_v1763_n2_p238_Pregi_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Apoptosis Bcl-X Erythropoietin Neuroprotection SH-SY5Y cell Staurosporine erythropoietin protein bcl xl staurosporine apoptosis article cell death cell differentiation cell growth cell structure controlled study human human cell immunoprecipitation neurite neuroblastoma cell priority journal reverse transcription polymerase chain reaction Western blotting Apoptosis bcl-X Protein Blotting, Western Cell Differentiation Dose-Response Relationship, Drug Electrophoresis Enzyme Inhibitors Erythropoietin Erythropoietin, Recombinant Humans Kinetics Microscopy, Fluorescence Neuroblastoma Precipitin Tests Receptors, Erythropoietin Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Staurosporine |
| spellingShingle |
Apoptosis Bcl-X Erythropoietin Neuroprotection SH-SY5Y cell Staurosporine erythropoietin protein bcl xl staurosporine apoptosis article cell death cell differentiation cell growth cell structure controlled study human human cell immunoprecipitation neurite neuroblastoma cell priority journal reverse transcription polymerase chain reaction Western blotting Apoptosis bcl-X Protein Blotting, Western Cell Differentiation Dose-Response Relationship, Drug Electrophoresis Enzyme Inhibitors Erythropoietin Erythropoietin, Recombinant Humans Kinetics Microscopy, Fluorescence Neuroblastoma Precipitin Tests Receptors, Erythropoietin Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Staurosporine Pregi, N. Vittori, D. Pérez, G. Leirós, C.P. Nesse, A. Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells |
| topic_facet |
Apoptosis Bcl-X Erythropoietin Neuroprotection SH-SY5Y cell Staurosporine erythropoietin protein bcl xl staurosporine apoptosis article cell death cell differentiation cell growth cell structure controlled study human human cell immunoprecipitation neurite neuroblastoma cell priority journal reverse transcription polymerase chain reaction Western blotting Apoptosis bcl-X Protein Blotting, Western Cell Differentiation Dose-Response Relationship, Drug Electrophoresis Enzyme Inhibitors Erythropoietin Erythropoietin, Recombinant Humans Kinetics Microscopy, Fluorescence Neuroblastoma Precipitin Tests Receptors, Erythropoietin Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Staurosporine |
| description |
Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved. |
| format |
Artículo Artículo publishedVersion |
| author |
Pregi, N. Vittori, D. Pérez, G. Leirós, C.P. Nesse, A. |
| author_facet |
Pregi, N. Vittori, D. Pérez, G. Leirós, C.P. Nesse, A. |
| author_sort |
Pregi, N. |
| title |
Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells |
| title_short |
Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells |
| title_full |
Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells |
| title_fullStr |
Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells |
| title_full_unstemmed |
Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells |
| title_sort |
effect of erythropoietin on staurosporine-induced apoptosis and differentiation of sh-sy5y neuroblastoma cells |
| publishDate |
2006 |
| url |
http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01674889_v1763_n2_p238_Pregi_oai |
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| _version_ |
1809357091882663936 |